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Immunological detection of Phaeoacremonium aleophilum, a fungal pathogen found in esca disease

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Abstract

Phaeoacremonium aleophilum is a fungal pathogen implicated in esca, a devastating disease affecting grapevines around the world. We have previously reported that the fungus secreted in its culture medium a variety of polypeptides, which allowed us to develop a serological method of detection. In this method, rabbit antibodies raised against the polypeptide fraction recognized secreted fungal proteins with high sensitivity (commonly 1 ng). These antibodies were specific since they cross-reacted with polypeptides secreted by various strains of Pm. aleophilum, but not with the proteins secreted by many other fungal pathogens implicated in other grapevine infections. Importantly, as shown by ELISA tests and immunolocalization, they did not cross-react with the secreted polypeptides of Eutypa lata (agent of Eutypa dieback), Neofusicoccum parvum (found in Black Dead Arm), and Phaeomoniella chlamydospora (also found in the fungal complex inducing esca). This serological approach permitted the development of a reliable dot-blot method to detect the presence in woody members of Pm. aleophilum in selectively infected trunk and canes. This method of diagnosis is rapid, easy to perform and non destructive for grapevines. Advantages and drawbacks of the method are discussed.

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Acknowledgments

The authors acknowledge the financial support given to this work by the Bureau National Interprofessionnel du Cognac. They are grateful to Image UP (Service de Microscopie de l’Université de Poitiers). The fungal strains were generously provided by Dr. P. Larignon (Institut Français de la Vigne et du Vin, Rodilhan).

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Authors and Affiliations

  1. Université de Poitiers, EBI UMR CNRS 7267, Laboratoire Physiologie Moléculaire du Transport des Sucres, 3 Rue Jacques Fort, 86022, Poitiers Cedex, France

    Pierrette Fleurat-Lessard, Estelle Luini & Gabriel Roblin

  2. Université de Poitiers, EBI UMR CNRS 7267, Laboratoire Microbiologie de l’eau, 40 Avenue du Recteur Pineau, 86022, Poitiers Cedex, France

    Jean-Marc Berjeaud

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  1. Pierrette Fleurat-Lessard
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  2. Estelle Luini
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  3. Jean-Marc Berjeaud
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  4. Gabriel Roblin
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Correspondence to Jean-Marc Berjeaud.

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Fleurat-Lessard, P., Luini, E., Berjeaud, JM. et al. Immunological detection of Phaeoacremonium aleophilum, a fungal pathogen found in esca disease. Eur J Plant Pathol 139, 137–150 (2014). https://doi.org/10.1007/s10658-013-0372-7

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