Abstract
In October 2016, we collected samples of post-harvest anthracnose of papaya from Gangneung, Gangwon Province, Korea. Infected fruits first showed tiny light brown spots on their surface followed by development of sunken water-soaked lesions with black acervuli. Fungal pathogen was isolated from the infected fruits and cultured on potato dextrose agar (PDA) and synthetic nutrient agar (SNA) medium. For identification of the fungus, morphological assessment and sequencing analysis of 5.8S ribosomal DNA (ITS-5.8S rDNA) and three protein-coding genes: actin (ACT), chitin synthase I (CHS-1), and glyceraldehyde-3-phosphate dehydrogenase (G3PDH) were performed. Pathogenicity of the fungal isolate was confirmed according to Koch’s postulates. The results of morphological examination, pathogenicity tests, and the ITS-5.8S rDNA, ACT, CHS-1, and G3PDH sequences confirmed Colletotrichum truncatum as the causal agent. This study provides news information about C. truncatum isolated from post-harvest papaya in Korea.
References
Arras, G., & Maltoni, S. L. (2004). Postharvest biological and integrated control of fungal pathogens on fruit. In R. Dris, R. Niskanen, & S. M. Jain (Eds.), Crop management and postharvest handling of horticultural products (pp. 115–183). USA: Science Publishers, Inc..
Carbone, I., & Kohn, L. M. (1999). A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia, 91(3), 553–556.
Damm, U., Woudenberg, J. H. C., Cannon, P. F., & Crous, P. W. (2009). Colletotrichum species with curved conidia from herbaceous hosts. Fungal Diversity, 39, 45–87.
FAO. (2013). Food and Agriculture Organization of the United Nations, FAOSTAT, FAO Statistical Databases, http://www.fao.org/waicent/portal/statistics_en.asp.
Farr, D. F., & Rossman, A. Y. (2017). Fungal Databases, Syst. Mycol. Microbiol. Lab., ARS, USDA. Retrieved from http://nt.ars-grin.gov/fungaldatabases/.
Freeman, S., Katan, T., & Shabi, E. (1998). Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Disease, 82, 596–605.
Kanchana-Udomkan, C., Taylor, P. W. J., & Mongkolporn, O. (2004). Development of a bioassay to study anthracnose infection of Capsicumm chinense Jacq. Fruit caused by Colletotrichum capsici. Thai Journal of Agriculture Science, 37, 293–297.
Morton, J. F. (1987). Papaya Carica papaya L. In J. F. Morton (Ed.), Fruits of Warm Climates (pp. 336–346). Miami: Creative Resource Systems.
Nakasone, H. Y., & Paull, R. E. (1998). Tropical fruits. Wallingford: CAB International.
O’Connell, R. J., Thon, M. R., Hacquard, S., Amyotte, S. G., Kleemann, J., & Torres, M. F. (2012). Life style transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses. Nature Genetics, 44, 1060–1056.
Saitou, N., & Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425.
Shenoy, B. D., Jeewon, R., Lam, W. H., Bhat, D. J., Than, P. P., Taylor, P. W. J., & Hyde, K. D. (2007). Morpho-molecular characterisation and epitypification of Colletotrichum capsici (Glomerellaceae, Sordariomycetes), the causal agent of anthracnose in chilli. Fungal Diversity, 27, 197–211.
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
Templeton, M. D., Rikkerink, E. H. A., Solon, S. L., & Crowhurst, R. N. (1992). Cloning and molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase encoding gene and cDNA from the plant pathogenic fungus Glomerella cingulata. Gene, 122, 225–230.
Than, P. P., Jeewon, R., Hyde, K. D., Pongsupasamit, S., Mongkolporn, O., & Taylor, P. W. J. (2008). Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathology, 57, 562–572.
White, T. J., Bruns, T. D., Lee, S. B., & Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). New York: Academic Press.
Yang, Y. L. (2009). Colletotrichum anthracnose of Amaryllidaceae. Fungal Diversity, 39, 123–146.
Acknowledgments
This research was supported by Golden Seed Project Grant (213001-04-4-SB630), Ministry of Agriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA) and Korean Forest Service (KFS), South Korea.
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Aktaruzzaman, M., Afroz, T., Lee, YG. et al. Post-harvest anthracnose of papaya caused by Colletotrichum truncatum in Korea. Eur J Plant Pathol 150, 259–265 (2018). https://doi.org/10.1007/s10658-017-1265-y
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DOI: https://doi.org/10.1007/s10658-017-1265-y