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Identification of Caragana arborescens shoot blight disease caused by Phaeobotryon caraganae sp. nov. (Botryosphaeriales) in China

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Abstract

In Harbin, Heilongjiang, China, a shoot blight of Caragana arborescens exhibiting light brown-to-brown sunken lesions with immersed fruiting bodies was observed during May through October of 2017. A survey was conducted to determine the extent and severity of the disease. In May 2017, a total of 12 diseased C. arborescens trees were found among 80 samples, with a field infection rate of 15%. Twelve random branches showing symptoms were collected and examined for symptoms and signs. Phylogenetic analysis, morphology, and isolate pathogenicity towards C. arborescens after inoculation of 20 healthy branches with spore suspensions revealed a new pathogenic species causing the shoot disease, which is described here as Phaeobotryon caraganae. P. caraganae is a new species and causes C. arborescens shoot disease. Future research on Phaeobotryon is important to improve our understanding of shoot diseases.

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References

  • Abdollahzadeh, J., Goltapeh, E. M., Javadi, A., Shams-bakhsh, M., Zare, R., & Phillips, A. J. L. (2009). Barriopsis iraniana and Phaeobotryon cupressi: two new species of the Botryosphaeriaceae from trees in Iran. Persoonia, 23, 1–8.

    Article  CAS  Google Scholar 

  • Alves, A., Crous, P. W., Correia, A., & Phillips, A. J. L. (2008). Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity, 28, 1–13.

    Google Scholar 

  • Arx, J. A. von., & Müller, E. (1954). Die Gattungen der amerosporen Pyrenomyceten. Beiträge zur Kryptogamenflora der Schweiz, 11(1),1–434.

  • Chai, R. Y., & Jin, M. Z. (1991). Describes a method for direct selection of monospora to obtain monospora strains. Plant Protection, 3, 53–54.

    Google Scholar 

  • Chen, J., Liu, X., Jia, H., & Zhu, W. (2018). First report of leaf-spot disease caused by Sphaeropsis visci on Asian mistletoe [Viscum coloratum (Kom.) Nakai] in China. Journal of Forestry Research, 29(6), 1769–1774.

    Article  Google Scholar 

  • Cooke, M. C. (1885). Synopsis pyrenomycetum. Grevillea, 13(67), 61–72.

    Google Scholar 

  • Daranagama, D. A., Thambugala, K. M., Campino, B., Alves, A., Bulgakov, T. S., Phillips, A. J. L., Liu, X. Z., & Hyde, K. D. (2016). Phaeobotryon negundinis sp. nov. (Botryosphaeriales) from Russia. Mycosphere, 7(7SI), 933–941.

    Article  Google Scholar 

  • Fan, X., Hyde, K. D., Liu, J., Liang, Y., & Tian, C. (2015). Multigene phylogeny and morphology reveal Phaeobotryon rhois sp. nov. (Botryosphaeriales, Ascomycota). Phytotaxa, 205(2), 90–98.

    Article  Google Scholar 

  • Fu, K., He, Y., & He, S. (1993). Flora of China. Science Press. Beijing, 42(1), 40.

    Google Scholar 

  • Grover, D. W., & Nicole, J. M. (1940). The vapour pressure of glycerin solutions at twenty degrees. Journal of the Society of Chemical Industry, 59, 175–177.

    CAS  Google Scholar 

  • Hillis, D. M., & Bull, J. J. (1993). An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42, 182–192.

    Article  Google Scholar 

  • Kochman, J., & Sikora, A. (2010). Experiments with a new virus disease of the pea tree (Caragana arborescens Lam.). Journal of Phytopathology, 61(2), 147–156.

    Article  Google Scholar 

  • Li, P. (2009). Application of Caragana in urban greening. China Urban Forestry, 7(06), 70–71.

    Google Scholar 

  • Liu, J. K., Phookamsak, R., Doilom, M., Wikee, S., Li, Y. M., Ariyawansha, H., Boonmee, S., Chomnunti, P., Dai, D. Q., Bhat, J. D., Romero, A. I., Zhuang, W. Y., Monkai, J., Jones, E. B. G., Chukeatirote, E., KoKo, T. W., Zhao, Y. C., Wang, Y., & Hyde, K. D. (2012). Towards a natural classification of Botryosphaeriales. Fungal Diversity, 57, 149–210.

    Article  Google Scholar 

  • Pavlic, D., Slippers, B., Coutinho, T. A., Gryzenhout, M., & Wingfield, M. J. (2004). Lasiodiplodia gonubiensis sp. nov., a new Botryosphaeria anamorph from native Syzygium cordatum in South Africa. Studies in Mycology, 50, 313–322.

    Google Scholar 

  • Phillips, A., Alves, A., Correia, A., & Luque, J. (2005). Two new species of Botryosphaeria with brown, 1-septate ascospores and Dothiorella anamorphs. Mycologia, 97(2), 513–529.

    Article  Google Scholar 

  • Phillips, A. J. L., Alves, A., Pennycook, S. R., Johnston, P. R., Ramaley, A., Akulov, A., & Crous, P. W. (2008). Resolving the phylogenetic and taxonomic status of dark-spored teleomorph genera in the Botryosphaeriaceae. Persoonia, 21, 29–55.

    Article  CAS  Google Scholar 

  • Phillips, A. J. L., Alves, A., Abdollahzadeh, J., Slippers, B., Wingfield, M. J., Groenewald, J. Z., & Crous, P. W. (2013). The Botryosphaeriaceae: genera and species known from culture. Studies in Mycology, 76, 51–167.

    Article  CAS  Google Scholar 

  • Schoch, C. L., Shoemaker, R. A., Seifert, K. A., Hambleton, S., Spatafora, J. W., & Crous, P. W. (2006). A multigene phylogeny of the Dothideomycetes using four nuclear loci. Mycology, 98(6), 1041–1052.

    Article  CAS  Google Scholar 

  • Slippers, B., Smit, W. A., Crous, P. W., Coutinho, T. A., Wingfield, B. D., & Wingfield, M. J. (2007). Taxonomy, phylogeny and identification of Botryosphaeriaceae associated with pome and stone fruit trees in South Africa and other regions of the world. Plant Pathology, 56(1), 128–139.

    Article  CAS  Google Scholar 

  • Stanosz, G., Smith, D., & Bernier, L. (2010). First report of Erysiphe palczewskii powdery mildew of siberian pea tree (Caragana arborescens) in Wisconsin and Quebec. Plant Disease, 93(12), 1352–1353.

    Article  Google Scholar 

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725–2729.

    Article  CAS  Google Scholar 

  • Theissen, F., & Sydow, H. (1915). Die Dothideales. Kritisch-systematische Originaluntersuchungen. Annales Mycologici, 13, 431–746.

    Google Scholar 

  • White, T. J., Bruns, T., & Lee, S. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis (Ed.), PCR protocol: A guide to methods and applications (pp. 315–322). New York: Academic Press.

    Google Scholar 

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Acknowledgements

This work was supported by grants from the National Key Research and Development Program of China (2017YFD0600101). I am grateful to all my colleagues who assisted in writing this thesis, particularly my supervisor, Dr. Xuefeng Liu, who offered me valuable suggestions during my academic study.

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Correspondence to Xuefeng Liu.

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Chen, J., Hao, X., Liu, X. et al. Identification of Caragana arborescens shoot blight disease caused by Phaeobotryon caraganae sp. nov. (Botryosphaeriales) in China. Eur J Plant Pathol 155, 537–544 (2019). https://doi.org/10.1007/s10658-019-01789-w

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