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Diversity in Puccinia graminis f. sp. avenae and its impact on oat cultivar response in South Africa

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Abstract

Puccinia graminis f. sp. avenae (Pga) samples were collected from cultivated oat (Avena sativa L.) in trial plots and commercial fields and from wild oat (Avena spp.) during the 2016 and 2017 seasons. Field samples were purified through selection of single pustule isolates and subsequent urediniospore increases using the susceptible cultivar Swan. For race phenotyping, a set of 12 international differential lines was used that resulted in the identification of races RSJ, RJS and RJJ. Race RSJ was the most prevalent (60% of isolates) followed by races RJS (34%) and RJJ (6%). These races varied in virulence for Pg6 and Pg12, with no avirulence recorded for Pg1, 2, 4, 8, 9, 13 and Pg15 and no virulence for Pg3, 10 and Pg16. Seedling infection types of 32 oat cultivars to the three described races showed compatible phenotypes for all cultivars towards at least one of the races. Moderate levels of adult plant resistance against races RJS and RSJ were recorded for seven cultivars during field tests in 2017 and 2018. Thirteen microsatellite markers used to assess genetic variability amongst twenty four field isolates revealed three sub-populations of which only one correlated with the race phenotype. The results contributed to our understanding of the diversity of the Pga population in South Africa, including useful resistance sources and cultivar responses.

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Acknowledgements

Producers and dealers of oat seed in SA (Agricultural Research Council – Small Grain, Agricol, Barenbrug, Capstone, K2, Pannar Seed and Sensako) are acknowledged for making seed of their cultivars available. Pannar Seed is thanked for the maintenance of field trials. Dr. Tom Fetch (Agriculture and Agri-Food Canada) and Prof Robert Park (Plant Breeding Institute, The University of Sydney) are thanked for providing seed of differential lines.

Funding

The National Research Foundation (SARChI chair UID 8464) is thanked for funding.

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Authors and Affiliations

  1. Department of Plant Sciences, University of the Free State, P. O. Box 339, Bloemfontein, 9300, South Africa

    W. H. P. Boshoff, B. Visser & Z. A. Pretorius

  2. Agricultural Research Council - Small Grain, Private Bag X29, Bethlehem, 9700, South Africa

    T. Terefe

Authors
  1. W. H. P. Boshoff
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  2. B. Visser
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  3. T. Terefe
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  4. Z. A. Pretorius
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Correspondence to W. H. P. Boshoff.

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We hereby declare that our submission is in compliance with the ethical responsibilities and standards of the EJPP as well as those of the University of the Free State.

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Boshoff, W.H.P., Visser, B., Terefe, T. et al. Diversity in Puccinia graminis f. sp. avenae and its impact on oat cultivar response in South Africa. Eur J Plant Pathol 155, 1165–1177 (2019). https://doi.org/10.1007/s10658-019-01845-5

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  • DOI: https://doi.org/10.1007/s10658-019-01845-5

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