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Effects of Baicalin and Octreotide on the Serum TNF-α Level and Apoptosis in Multiple Organs of Rats with Severe Acute Pancreatitis

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Abstract

We investigated the effects of Baicalin and Octreotide on the levels of endotoxin and TNF-α in blood and the effects of apoptotic changes in multiple organs of SAP rats, and explored the underlying therapeutic mechanisms of Baicalin and Octreotide. In this study, 135 SAP rats were randomly divided into model control, Baicalin treated and Octreotide treated group (n = 45), respectively, the same number of normal rats were included in sham-operated group (n = 45). The above-mentioned groups were further subdivided into 3, 6 and 12 h subgroups, respectively (15 rats in each subgroup). At 3, 6 and 12 h after operation, the mortality rate of rats, endotoxin and TNF-α levels in blood as well as the pathological severity scores, expression levels of Bax protein and apoptosis indexes in multiple organs were determined. Compared to model control group (1),both drugs can relieve the pathological injuries of multiple organs and decrease significantly the levels of endotoxin and TNF-α in blood and the mortality rate of rats in treated groups (P < 0.05 or P < 0.01); (2) the expression of Bax protein was upregulated in pancreas, lung, intestinal mucosa (P < 0.05 or P < 0.01) but downregulated in spleen and lymph nodes (P < 0.001 and P < 0.05, respectively) in Baicalin treated group; The apoptosis indexes significantly increased in pancreas, intestinal mucosa, lymph nodes and spleen (P < 0.05 or P < 0.01). (3) the expression of Bax protein was upregulated in pancreas and lung but downregulated in spleen and lymph nodes (P < 0.05 or P < 0.01) in Octreotide treated group; The apoptosis indexes significantly increased in lymph nodes and spleen in Octreotide treated group (P < 0.05 or P < 0.01). Baicalin and Octreotide share a similar therapeutic efficacy in the treatment of SAP via a mechanism that is associated with inhibiting the levels of TNF-α in blood and induce apoptosis in multiple organs.

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Authors and Affiliations

  1. Department of General Surgery, Affiliated Xiaoshan First People’s Hospital, Hangzhou Normal University, Hangzhou, 311200, Zhejiang Province, China

    Tian Hua, Ying Rongchao & Ye Jing

  2. Department of General Surgery, Hangzhou First People’s Hospital, Hangzhou, 310006, Zhejiang Province, China

    Zhang Xiping

  3. Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310053, Zhejiang Province, China

    Wu Chengjun, Yu Bingyan, Ye Qian, Pan Yan, Ma Meili & Zhu Feibo

  4. Department of General Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, Zhejiang Province, China

    Chen Li

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  1. Tian Hua
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Correspondence to Zhang Xiping.

Additional information

We claimed that this paper was original and would not have any financial interest in a company or its competitor, and that all authors meet criteria for authorship. We abided the ethics in this animal experiment study. The ethics committee approval of our hospital was secured for the animal study reported, and all rats have not been abused and executive mercy killing when the observing time in this study was over. As a corresponding author, Zhang Xiping takes the equal task to Tian Hua in this study. The levels of TNF-α and Endotoxin can be used as important markers for assessing the severity of multiple organ injury in SAP, so we repeated the data in our manuscript although they have been published in other journals.

Supported by technological foundation project of Traditional Chinese Medicine Science of Zhejiang province (NO.2003C130; NO.2004C142), foundation project for medical science and technology of Zhejiang province (No.2003B134), grave foundation project for technological and development of Hangzhou (NO.2003123B19), intensive foundation project for technology of Hangzhou (NO.2004Z006), foundation project for medical science and technology of Hangzhou (No.2003A004) and foundation project for technology of Hangzhou (No.2005224).

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Hua, T., Xiping, Z., Chengjun, W. et al. Effects of Baicalin and Octreotide on the Serum TNF-α Level and Apoptosis in Multiple Organs of Rats with Severe Acute Pancreatitis. Inflammation 32, 191–201 (2009). https://doi.org/10.1007/s10753-009-9120-8

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