Abstract
In the current examination, we have built up a novel, green approach for the synthesis of Ag NPs (silver nanoparticles) from Rauvolfia tetraphylla leaves extract. The synthesized Ag NPs were thoroughly characterized using different analytical techniques like Powder X-ray diffraction, Fourier transform infrared spectroscopy, UV–visible spectroscopy, scanning electron microscopy and transmission electron microscope analysis. It is confirmed as a cubic phased silver nanoparticle with an average particle size of around 40 nm with a spherical shape. Further, the characterized material was examined for antioxidant activity and it has shown the IC50 (inhibitory concentration 50%) value of 82.13 µg/mL against the scavenging of DPPH free radical. The cytogenetic effect of silver nanoparticles was tested on the root cells of Allium cepa, from this examination we have noted the antimitotic activity and precise chromosomal aberrations such as chromosome-breaks, chromosome-stickiness, laggard chromosome, clumped chromosome etc. By discharging Ag+ ions and producing ROS, silver nanoparticle exhibits great anticancer activity. Therefore, this paperwork effectively shows the synthesis of Ag nanoparticles by simple eco-friendly green way utilizing Rauvolfia tetraphylla leaves to extract as a green reducing agent as well as stabilizing/capping agent for the synthesis of Ag NPs.
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Acknowledgements
Udayabhanu thanks to CSIR, New Delhi, for Senior Research Fellowship (09/1204(0001)/2018-EMR-1). Dr. Chandrasekhar thanks to Dr. M R Hulinaykar, Managing Trustee, SIET, Tumakuru. Dr. GN Thank DST Nanomission (SR/NM/NS-1262/2013) for financial support. We acknowledge Dr. M. Veerabhadrayya, from Tumkur University, for helped us towards Rietveld refinement studies. We acknowledge Dr. M. Govindappa, from Dayananda Sagar College of Engineering, Bengaluru, for encouragement during this research work.
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Vinay, S.P., Udayabhanu, Nagaraju, G. et al. Rauvolfia tetraphylla (Devil Pepper)-Mediated Green Synthesis of Ag Nanoparticles: Applications to Anticancer, Antioxidant and Antimitotic. J Clust Sci 30, 1545–1564 (2019). https://doi.org/10.1007/s10876-019-01598-5
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DOI: https://doi.org/10.1007/s10876-019-01598-5