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Eupenicillium saturniforme, a New Species Discovered from Northeast China

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Abstract

A new Eupenicillium species, E. saturniforme was isolated from soil in Jilin Province, northeast China. Morphologically, it resembled E. shearii and E. tropicum, but is distinguished from them by slowly maturing cleistothecia, lenticular ascospores with nearly smooth-walled convex surfaces, strictly velutinous colony texture with abundant conidiogenesis, robust biverticillate penicilli, apically vesiculate metulae and rough-walled stipes and conidia. The partial β-tubulin gene sequence of the new species (EU644080) showed relationship with Penicillium glabrum in the BLAST search in GenBank. Further analyses of partial calmodulin and ribosomal DNA internally transcribed spacer 1-5.8S-internally transcribed spacer 2 (rDNA ITS1-5.8S-ITS2) sequence data confirmed that E. saturniforme fell in the clade with P. glabrum, P. lividum, P. purpurascens, P. spinulosum and P. thomii of Subgenus Aspergilloides. However, E. saturniforme is a distinctive species lacking close relatives among described species of penicillia.

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References

  1. Calvo AM, Wilson RA, Bok JW, Keller NP. Relationship between secondary metabolism and fungal development. Microbiol Mol Biol Rev. 2002;66:447–59. doi:10.1128/MMBR.66.3.447-459.2002.

    Article  PubMed  CAS  Google Scholar 

  2. Han D-M, Chae K-S, Han K-H. Sexual development in Aspergillus nidulans. In: Goldman GH, Osmani SA, editors. The aspergilli genomics, medical aspects, biotechnology, and research methods. Boca Raton: CRC Press; 2008. p. 279–99.

    Google Scholar 

  3. Stolk AC, Samson RA. The ascomycete genus Eupenicillium and related Penicillium anamorphs. Stud Mycol. 1983;23:1–149.

    Google Scholar 

  4. Scott DeB. The genus Eupenicillium Ludwig. Res Rep Coun Scient Ind Res Pretoria. 1968;272:1–150.

    Google Scholar 

  5. Wen DC. The chorography of Dunhua. Beijing: Xinhua Press; 1991 (in Chinese).

    Google Scholar 

  6. Pitt JI, Samson RA, Frisvad JC. List of accepted species and their synonyms in the family Trichocomaceae. In: Samson RA, Pitt JI, editors. Integration of modern taxonomic methods for Penicillium and Aspergillus classification. Amsterdam: Harwood Academic Publishers; 2000. p. 9–49.

    Google Scholar 

  7. Ueda S. A new species of Eupenicillium from marine sediment. Mycoscience. 1995;36:451–4. doi:10.1007/BF02268631.

    Article  Google Scholar 

  8. Tuthill DE, Frisvad JC. Eupenicillium bovifimosum, a new species from dry cow manure in Wyoming. Mycologia. 2002;94:240–6. doi:10.2307/3761800.

    Article  Google Scholar 

  9. Tuthill DE, Frisvad JC. A new species from tropical soils, Eupenicillium tropicum. Mycol Prog. 2004;3:13–8. doi:10.1007/s11557-006-0071-2.

    Article  Google Scholar 

  10. Pitt JI. The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. London: Academic Press; 1980 [“1979”].

    Google Scholar 

  11. Malloch D. Moulds their isolation, cultivation and identification. Toronto: University of Toronto Press; 1981.

    Google Scholar 

  12. Peterson SW. Phylogenetic analysis of Penicillium species based on ITS and lsu-rDNA nucleotide sequences. In: Samson RA, Pitt JI, editors. Integration of modern taxonomic methods for Penicillium and Aspergillus classification. Amsterdam: Harwood Academic Publishers; 2000. p. 163–78.

    Google Scholar 

  13. Frisvad JC, Samson RA. Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and air-borne terverticillate penicillia and their mycotoxins. Stud Mycol. 2004;49:1–173. doi:10.1016/S0922-5382(04)80002-3.

    Article  Google Scholar 

  14. Raper KB, Thom C. A Manual of the Penicillia. Baltimore, MD: Williams and Wilkins Co; 1949.

    Google Scholar 

  15. Ridgway R. Color standards and color nomenclature. Washington, DC: The Author; 1912.

  16. Scott J, Malloch D, Wong B, Sawa T, Straus N. DNA heteroduplex fingerprinting in Penicillium. In: Samson RA, Pitt JI, editors. Integration of modern taxonomic methods for Penicillium and Aspergillus classification. Amsterdam: Harwood Academic Publishers; 2000. p. 225–36.

    Google Scholar 

  17. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous Ascomycetes. Appl Environ Microbiol. 1995;61:1323–30.

    PubMed  CAS  Google Scholar 

  18. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocols: a guide to methods and applications. San Diego, CA: Academic Press; 1990. p. 315–22.

    Google Scholar 

  19. Wang L, Zhuang W-Y. Designing primer sets for amplification of partial calmodulin genes from penicillia. Mycosystema. 2004;23:466–73.

    CAS  Google Scholar 

  20. Wang L, Zhuang W-Y. Phylogenetic analyses of penicillia based on partial calmodulin gene sequences. Biosystems. 2007;88:113–26. doi:10.1016/j.biosystems.2006.04.008.

    Article  PubMed  CAS  Google Scholar 

  21. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser. 1999;41:95–8.

    CAS  Google Scholar 

  22. Thompson JD, Gibbson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res. 1997;25:4876–82. doi:10.1093/nar/25.24.4876.

    Article  PubMed  CAS  Google Scholar 

  23. Malloch D. The Trichocomaceae: relationships with other ascomycetes. In: Samson RA, Pitt JI, editors. Advances in Penicillium and Aspergillus systematics. New York: Plenum Press; 1985. p. 365–82.

    Google Scholar 

  24. Berbee ML, Yoshimura A, Sugiyama J, Taylor JW. Is Penicillium monophyletic? An evaluation of phylogeny in the family Trichocomaceae from 18S, 5.8S and ITS ribosomal DNA sequence data. Mycologia. 1995;87:210–22. doi:10.2307/3760907.

    Article  CAS  Google Scholar 

  25. Swofford DL. PAUP*: Phylogenetic analysis using parsimony (*and other methods), version 4. Sunderland, MA: Sinauer Associates; 2001.

    Google Scholar 

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Acknowledgements

We thank Jian-Yun Zhuang for reviewing the Latin diagnosis. This study was supported by National Natural Science Foundation of China through Grant nos. 30500002 to L.W. and 30499340 and W-Y.Z., respectively, and the Knowledge Innovation Program of Chinese Academy of Sciences under Grant no. KSCX2-YW-Z-043 to L.W.

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  1. Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Long Wang & Wen-Ying Zhuang

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  1. Long Wang
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  2. Wen-Ying Zhuang
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Correspondence to Long Wang.

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Supplementary Fig. 1

The NJ tree inferred from the rDNA ITS1-5.8S-ITS2 sequence data set. The bootstrap percentages over 50% derived from 1000 replicates were indicated at the nodes (DOC 86 kb)

Supplementary Fig. 2

One of four equally parsimonious trees generated by a heuristic search of the partial sequences of calmodulin gene data set using PAUP 4.0b8a in random sequence addition order. (Tree Length = 2033, CI = 0.4058, HI = 0.5942, RI = 0.7499, RC = 0.3043). The bootstrap percentages over 50% derived from 1000 replicates were indicated at the nodes (DOC 95 kb)

Supplementary Fig. 3

One of 943 equally parsimonious trees generated by a heuristic search of the rDNA ITS1-5.8S-ITS2 sequence data set using PAUP 4.0b8a in random sequence addition order. (Tree Length = 579, CI = 0.5043, HI = 0.4957, RI = 0.7023, RC = 0.3542). The bootstrap percentages over 50% derived from 1000 replicates were indicated at the nodes (DOC 86 kb)

Supplementary Data set 1

The 715-character alignment of partial calmodulin gene sequences (PDF 99 kb)

Supplementary Data set 2

The 592-character alignment of rDNA ITS1-5.8S-ITS2 sequences (PDF 87 kb)

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Wang, L., Zhuang, WY. Eupenicillium saturniforme, a New Species Discovered from Northeast China. Mycopathologia 167, 297–305 (2009). https://doi.org/10.1007/s11046-008-9179-z

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  • DOI: https://doi.org/10.1007/s11046-008-9179-z

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