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Reclassification of Two Peronospora Species Parasitic on Draba in Hyaloperonospora Based on Morphological and Molecular Phylogenetic Data

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Abstract

On the family Brassicaceae, the causal agent responsible for downy mildew disease was originally regarded as a single species, Peronospora parasitica (now under Hyaloperonospora), but it was recently reconsidered to consist of many distinct species. In this study, 11 specimens of Peronospora drabae and P. norvegica parasitic on the genus Draba were investigated morphologically and molecularly. Pronounced differences in conidial sizes (P. drabae: 14–20 × 12.5–15.5 μm; P. norvegica: 20–29 × 15.5–22 μm) and 7.8% sequence distance between their ITS1-5.8S-ITS2 rDNA sequences confirmed their status as distinct species. Based on ITS phylogeny and morphology (monopodially branching conidiophores, flexuous to sigmoid ultimate branchlets, hyaline conidia and lobate haustoria), the two species unequivocally belong to the genus Hyaloperonospora and not to Peronospora to which they were previously assigned. Therefore, two new combinations, Hyaloperonospora drabae and H. norvegica, are proposed. The two taxa are illustrated and compared using the type specimen for H. norvegica and authentic specimens for H. drabae, which is lectotypified.

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References

  1. Persoon CH. Observationes mycologicae. Vol. 1. Leipzig: P.P. Wolf; 1796.

    Google Scholar 

  2. Gäumann E. Über die Formen der Peronospora parasitica (Pers.) Fries. Ein Beitrag zur Speziesfrage bei den parasitischen Pilzen. Beih Bot Centralb. 1918;34(1):395–533.

    Google Scholar 

  3. Gäumann E. Beiträge zu einer Monographie der Gattung Peronospora Corda. Beitr Kryptogamenfl Schweiz. 1923;5(4):1–360.

    Google Scholar 

  4. Gustavsson A. Studies on nordic peronosporas. I. Taxonomic revision. Opera Bot. 1959;3(1):1–271.

    Google Scholar 

  5. Yerkes WD, Shaw CG. Taxonomy of the Peronospora species on Cruciferae and Chenopodiaceae. Phytopathology. 1959;49:499–507.

    Google Scholar 

  6. Constantinescu O, Fatehi J. Peronospora-like fungi (Chromista, Peronosporales) parasitic on Brassicaceae and related hosts. Nova Hedwigia. 2002;74:291–338.

    Article  Google Scholar 

  7. Riethmüller A, Voglmayr H, Göker M, Weiß M, Oberwinkler F. Phylogenetic relationships of the downy mildews (Peronosporales) and related groups based on nuclear large subunit ribosomal DNA sequences. Mycologia. 2002;94:834–49.

    Article  PubMed  Google Scholar 

  8. Choi YJ, Hong SB, Shin HD. Diversity of Hyaloperonospora parasitica complex from core brassicaceous hosts based on ITS rDNA sequence. Mycol Res. 2003;107:1313–21.

    Article  Google Scholar 

  9. Voglmayr H. Phylogenetic relationships of Peronospora and related genera based on nuclear ribosomal ITS sequences. Mycol Res. 2003;107:1132–42.

    Article  CAS  PubMed  Google Scholar 

  10. Göker M, Voglmayr H, Riethmüller A, Weiß M, Oberwinkler F. Taxonomic aspects of Peronosporaceae inferred from Bayesian molecular phylogenetics. Can J Bot. 2003;81:672–83.

    Article  Google Scholar 

  11. Göker M, Riethmüller A, Voglmayr H, Weiss M, Oberwinkler F. Phylogeny of Hyaloperonospora based on nuclear ribosomal internal transcribed spacer sequences. Mycol Prog. 2004;3:83–94.

    Article  Google Scholar 

  12. Göker M, Voglmayr H, García–Blázquez G, Oberwinkler F. Species delimitation in downy mildews: the case of Hyaloperonospora in the light of nuclear ribosomal ITS and LSU sequences. Mycol Res. 2009;113:308–25.

    Article  PubMed  Google Scholar 

  13. Shin HD, Choi YJ. A first check-list of Peronosporaceae from Korea. Mycotaxon. 2003;86:249–67.

    Google Scholar 

  14. Shin HD, Choi YJ. Peronosporaceae of Korea. Suwon, Korea: National Institute of Agricultural Science and Technology; 2006.

    Google Scholar 

  15. Holmgren PK, Holmgren NH. 1998. Index Herbariorum. New York Botanical Garden. http://sciweb.nybg.org/science2/indexHerbariorum.asp.

  16. Lee SB, Taylor JW. Isolation of DNA from fungal mycelia and single spores. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 282–7.

    Google Scholar 

  17. Cooke DEL, Drenth A, Duncan JM, Wagels G, Brasier M. A molecular phylogeny of Phytophthora and related Oomycetes. Fungal Genet Biol. 2000;30:17–32.

    Article  CAS  PubMed  Google Scholar 

  18. Moncalvo JM, Wang HH, Hseu RS. Phylogenetic relationships in Ganoderma inferred from the internal transcribed spacer and 25S ribosomal DNA sequences. Mycologia. 1995;87:223–38.

    Article  CAS  Google Scholar 

  19. Voglmayr H, Constantinescu O. Revision and reclassification of three Plasmopara species based on morphological and molecular phylogenetic data. Mycol Res. 2008;112:487–501.

    Article  CAS  PubMed  Google Scholar 

  20. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego: Academic Press; 1990. p. 315–22.

    Google Scholar 

  21. Voglmayr H, Jaklitsch W. Prosthecium species with Stegonsporium anamorphs on Acer. Mycol Res. 2008;112:885–905.

    Article  PubMed  Google Scholar 

  22. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;24:4876–82.

    Article  Google Scholar 

  23. Stamatakis E. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics. 2006;22:2688–90.

    Article  CAS  PubMed  Google Scholar 

  24. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596–9.

    Article  CAS  PubMed  Google Scholar 

  25. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39:783–91.

    Article  Google Scholar 

  26. Ronquist F, Huelsenbeck JP. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003;19:1572–4.

    Article  CAS  PubMed  Google Scholar 

  27. Göker M, Voglmayr H, Riethmüller A, Oberwinkler F. How do obligate parasites evolve? A multi-gene phylogenetic analysis of downy mildews. Fungal Genet Biol. 2007;44:105–22.

    Article  PubMed  Google Scholar 

  28. Thines M. Evaluation of characters available from herbarium vouchers for the phylogeny of the downy mildew genera (Chromista, Peronosporales), with focus on scanning electron microscopy. Mycotaxon. 2006;97:195–218.

    Google Scholar 

  29. Hamilton AJ, Cunnington JH. Calculating minimum sample sizes for taxonomic measurements: examples using Gäumann’s Peronospora spore data. Mycotaxon. 2006;95:189–94.

    Google Scholar 

  30. Choi YJ, Constantinescu O, Shin HD. A new downy-mildew of the Rosaceae: Peronospora oblatispora sp. nov. (Chromista, Peronosporales). Nova Hedwigia. 2007;85:93–101.

    Article  Google Scholar 

  31. Choi YJ, Hong SB, Shin HD. Re-consideration of Peronospora farinosa infecting Spinacia oleracea as distinct species, Peronospora effusa. Mycol Res. 2007;111:381–91.

    Article  CAS  PubMed  Google Scholar 

  32. Choi YJ, Denchev CM, Shin HD. Morphological and molecular analyses support the existence of host-specific Peronospora species infecting Chenopodium. Mycopathologia. 2008;168:155–64.

    Article  Google Scholar 

  33. Choi YJ, Shin HD, Thines M. Two novel Peronospora species are associated with recent reports of downy mildew on sages. Mycol Res. 2009;113:1340–50.

    Article  CAS  PubMed  Google Scholar 

  34. Kochman J, Majewski T. Grzyby (Mycota). Tom IV. Glonowce (Phycomycetes), Wroslikowe (Peronosporales). Państwowe Wydawnictwo Naukowe: Warzawa; 1970.

    Google Scholar 

  35. Ul’yanishchev VI, Osipian LL, Kanchaveli LA, Akhundov TM. Peronosporovye griby (Peronosporaceous Fungi). In: Ul’yanishchev VI, editor. Opredelitel’ gribov zakavkaz’ya: Erevan University, Erevan; 1985.

  36. Staneviciené S. Peronosporales. In: Mazelaitis J, Staneviciené S, editors. Lietavos Grybai. I. Gleivunai (Myxomycota), Peronosporieciai (Peronosporales) 1. Vilnius: Mokslo ir enciklopediju leidykla; 1995. p. 85–291.

    Google Scholar 

  37. Novotelnova NS, Pystina KA. Ordo Peronosporales (Fam. Pythiaceae, Phytophthoraceae, Peronosporaceae, Cystopaceae). In: Gorlenko MV, editor. Cryptogamic flora of URSS, vol, 11, Fungi (3). Leningrad: Nauka; 1985.

    Google Scholar 

  38. Rouppert K. Przyczynek do znajomości grzybów Galicyi i Bukowiny. Kosmos, Lwów. 1911;36:936–44.

    Google Scholar 

  39. Voglmayr H, Fatehi J, Constantinescu O. Revision of Plasmopara (Chromista, Peronosporales) parasitic on Geraniaceae. Mycol Res. 2006;110:633–45.

    Article  PubMed  Google Scholar 

  40. Thines M, Choi YJ, Kemen E, Ploch S, Holub EB, Shin HD, Jones JDG. A new species of Albugo parasitic to Arabidopsis thaliana reveals new evolutionary patterns in white blister rusts (Albuginaceae). Persoonia. 2009;22:123–8.

    CAS  PubMed  Google Scholar 

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Acknowledgments

The authors are grateful to the herbarium curators of the U.S. National Fungus Collections (BPI), the Royal Botanic Gardens Kew (K), the Botanical Museum Oslo (O), the Naturhistorisches Museum Wien (W) and the Universität Wien (WU) for providing the Peronospora specimens. Financial support via a research grant from the Korea University to H. D. S. in 2007 and a EU SYNTHESYS grant (GB-TAF-4625) to H. V. for a research stay at the Royal Botanic Gardens Kew are gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Environment and Ecology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

    Young-Joon Choi

  2. Division of Environmental Science and Ecological Engineering, Korea University, Seoul, 136-701, Korea

    Hyeon-Dong Shin

  3. Department of Systematic and Evolutionary Botany, Faculty Center Biodiversity, University of Vienna, Rennweg 14, 1030, Wien, Austria

    Hermann Voglmayr

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  1. Young-Joon Choi
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  2. Hyeon-Dong Shin
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  3. Hermann Voglmayr
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Correspondence to Hermann Voglmayr.

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Choi, YJ., Shin, HD. & Voglmayr, H. Reclassification of Two Peronospora Species Parasitic on Draba in Hyaloperonospora Based on Morphological and Molecular Phylogenetic Data. Mycopathologia 171, 151–159 (2011). https://doi.org/10.1007/s11046-010-9340-3

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  • DOI: https://doi.org/10.1007/s11046-010-9340-3

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