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Direct plant regeneration from nodal explants of Balanites aegyptiaca L. (Del.): a valuable medicinal tree

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Abstract

This report describes in vitro shoot induction and plant regeneration from nodal segments of Balanites aegyptiaca on Murashige and Skoog (MS) medium fortified with 6-benzyladenine (BA), thidiazuron (TDZ) and kinetin (Kin) (0.5–20.0 μM). MS medium supplemented with BA (12.5 μM) was the most effective in inducing bud break and growth and also in initiating multiple shoot proliferation. However, the optimal level of TDZ supplementation to the culture medium was 5.0 μM. Shoot cultures were established by repeatedly subculturing the original nodal explants on the same medium. Highest number of shoots (11.5 ± 0.7) and shoot length (5.0 ± 0.2 cm) were achieved when cultures were subcultured on MS medium supplemented with 12.5 μM BA and 1.0 μM α-naphthalene acetic acid (NAA). The shoots regenerated from TDZ supplemented medium when subcultured to hormone free MS basal medium considerably increased the rate of shoot multiplication and shoot length by the end of fifth subculture. Rooting of the shoots was achieved on MS medium augmented with 1.0 μM indole-3-butyric acid (IBA) plus 0.5% activated charcoal followed by their transfer to half strength MS basal medium. The in vitro raised plantlets with well developed shoots and roots were successfully established in earthen pots containing garden soil and were grown in greenhouse with 70% survival rate. The results of this study provide the first successful report on in vitro direct plant regeneration of B. aegyptiaca.

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Abbreviations

BA:

6-Benzyladenine

IBA:

Indole-3-butyric acid

Kin:

Kinetin

MS:

Murashige and Skoog medium

NAA:

α-Naphthalene acetic acid

TDZ:

Thidiazuron

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Acknowledgements

Authors gratefully acknowledge the University Grants Commission, New Delhi for financial assistance and the Department of Science and Technology, Govt. of India, New Delhi, for providing research support under DST-FIST Program. Thanks are also due to Dr. S. I. Ahmed, Arid Forest Research Institute (AFRI), Jodhpur for his help and cooperation.

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  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, India

    Iram Siddique & M. Anis

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  1. Iram Siddique
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  2. M. Anis
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Correspondence to M. Anis.

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Siddique, I., Anis, M. Direct plant regeneration from nodal explants of Balanites aegyptiaca L. (Del.): a valuable medicinal tree. New Forests 37, 53–62 (2009). https://doi.org/10.1007/s11056-008-9110-y

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  • DOI: https://doi.org/10.1007/s11056-008-9110-y

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