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Antioxidant and cytotoxic activity of lichens collected from Bidoup Nui Ba National Park, Vietnam

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Abstract

Lichens were some of the earliest colonizers of terrestrial habitats on Earth. They represent a unique symbiont between fungi (mycobionts) and algae and/or cyanobacteria (photobionts). Lichens have been used as a cure for diabetes, coughs, pulmonary tuberculosis, wound healing, and dermatological diseases. The aim of this study is to investigate the in vitro antioxidant and cytotoxic activities of methanol lichen extracts. Fourteen lichen species from Bidoup Nui Ba National Park were identified according to their morphology and anatomical and chemical characteristics. The antioxidant activity of the methanol lichen extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay. The results showed that, of the lichens tested, L5 had the highest free radical activity with 50% inhibitory concentration (IC50) of 59.9 ± 4.65 mg mL−1. The methanol extract of L5 also showed the highest total flavonoid and polyphenol contents. In a cytotoxic assay, it was observed that the methanol extract of U38.1 exhibited high cytotoxic effect against MCF-7 cells, with IC50 of 34.27 ± 1.25 mg mL−1. The tested lichen extracts were also found to have slight cytotoxic effect on fibroblasts at screening concentration of 100 µg mL−1. All of the extracts were found to possess different cytotoxic activities against MO-91 cells, with IC50 values ranging from 10.50 ± 1.56 to over 50 mg mL−1. All of the extracts except U38.1 induced normal peripheral blood mononuclear cell (PBMC) proliferation, especially after 48 h of treatment at 25 µg/mL.

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References

  1. J. Kumar, P. Dhar, A.B. Tayade, D. Gupta, O.P. Chaurasia, D.K. Upreti, R. Arora, R.B. Srivastava, PLoS ONE 9, 1 (2014)

    Google Scholar 

  2. M. Kosanić, B. Ranković, J. Sci. 32, 65–72 (2010)

    Google Scholar 

  3. B.C. Behera, N. Verma, A. Sonone, U. Makhija, J Biotechnol. Lett. 27, 991 (2005)

    Article  CAS  Google Scholar 

  4. J. Boustie, M. Grube, Plant Genet. Resour. 3, 273 (2005)

    Article  CAS  Google Scholar 

  5. Y.Q. Liu, X.Y. Hu, T. Lu, Y.N. Cheng, C.Y.F. Young, H.Q. Yuan, H.X. Lou, PLoS ONE 7, 1 (2012)

    Google Scholar 

  6. K. Müller, Appl. Microbiol. Biot. 56, 9 (2001)

    Article  Google Scholar 

  7. N.T. Manojlovic, P.J. Vasiljevic, P.Z. Maskovic, M. Juskovic, G.B. Dusanovic, Hindawi Publ. Corp. 2012, 1 (2012)

    Google Scholar 

  8. G. Shrestha, L.L.S. Clair, Phytochem. Rev. 12, 229 (2013)

    Article  CAS  Google Scholar 

  9. F. Brisdelli, M. Perilli, D. Sellitri, M. Piovano, J.A. Garbarino, M. Nicoletti, A. Bozzi, G. Amicosante, G. Celenza, Phytother Res. 27, 431 (2013)

    Article  CAS  PubMed  Google Scholar 

  10. B. Ranković, M. M. Kosanić, T. P Stanojković, BMC Complem. Altern. Med. 11, 1 (2011)

  11. N. Verma, B.C. Behera, U. Makhija, Appl. Biochem. Biotechnol. 151, 167 (2008)

    Article  CAS  PubMed  Google Scholar 

  12. U. Jayalal, S. Joshi, S.O. Oh, J.A. Kim, Y.J. Koh, F. Crian, J.S. Hur, Mycobiology 42, 6 (2013)

    Article  Google Scholar 

  13. D. Triggiani, D. Ceccarelli, A. Tiezzi, T. Pisani, S. Munzi, C. Gaggi, S. Loppi, Biologia 64, 59 (2009)

    Article  Google Scholar 

  14. U. Jayalal, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 124, 51 (2013)

    Article  Google Scholar 

  15. S. Joshi, U. Jayalal, N.T. Thuy, N.A. Dzung, J.S. Hur, The Lichenologist 45, 1 (2013)

    Article  Google Scholar 

  16. S. Joshi, U. Jayalal, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 123, 479 (2013)

    Article  Google Scholar 

  17. S. Joshi, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 124, 309 (2013)

    Article  Google Scholar 

  18. S. Joshi, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 125, 69–80 (2013)

    Article  Google Scholar 

  19. S. Joshi, N.T. Thuy, N.A. Dzung, J.S. Hur, Mycobiology 42, 17 (2014)

    Article  PubMed  PubMed Central  Google Scholar 

  20. S. Joshi, D. K. Upreti, S.O. Oh, · N. T. Thuy, N. A. Dzung, J. S. Hur, Mycotaxon. 130, 329 (2015)

  21. N.T. Thuy, N.A. Dzung, J.S. Hur, Taiwania 55, 402 (2010)

    Google Scholar 

  22. N.T. Thuy, N.A. Dzung, J.S. Hur, Mycotaxon 117, 93 (2011)

    Article  Google Scholar 

  23. J.A. Elix, K.D. Ernst-Russell, A Catalogue of Standardized Thin Layer Chromatographic Data and Biosynthetic Relationships for Lichen Substances, 2nd edn. (Australian National University, Canberra, 1993)

    Google Scholar 

  24. A. Orange, P.W. James, F.J. White, Microchemical Methods for the Identification of Lichens. British Lichen Society, London, UK, 101 (2001)

  25. U. Jayalal, P.K. Divakar, S. Joshi, S.O. Oh, Y.J. Koh, J.S. Hur, Mycobiology 41, 25 (2013)

    Article  PubMed  PubMed Central  Google Scholar 

  26. C.K. Lin, Coll. Res. 26, 37 (2013)

    Google Scholar 

  27. Y. Ohmura, Mem. Natl. Mus. Nat. Sci. 48, 91 (2012)

    Google Scholar 

  28. T. Randlane, T. TõRRa, A. Saag, L. Saag, Bibliotheca Lichenologica 100, 419 (2009)

    Google Scholar 

  29. H. Sipman, Mycotaxon 1, 235 (1986)

    Google Scholar 

  30. J. Zhishen, T. Mengcheng, W. Jianming, J. Food Chem. 64, 555 (1999)

    Article  CAS  Google Scholar 

  31. B.C. Sharma, S. Kalikotay, IOSR-PHR. 2, 54 (2012)

    Google Scholar 

  32. C.M. Liyana-Pathirana, F. Shahidi, J. Agric. Food Chem. 53, 2433 (2005)

    Article  CAS  PubMed  Google Scholar 

  33. B. Ranković, M. Kosanić, T. Stanojković, P. Vasiljević, N. Manojlović, Int. J. Mol. Sci. 13, 11 (2011)

    Google Scholar 

  34. S.Ç. Kasimoğullari, S. Oran, F. Ari, E. Ulukaya, N. Aztopal, M. Sarimahmut, Ş. Öztürk, Turk. J. Biol. 38, 940 (2014)

    Article  Google Scholar 

  35. N. Manojlovic, B. Rankovi, M. Kosani, P. Vasiljevi, T. Stanojkovi, Phytomedicine 19, 1166 (2012)

    Article  CAS  PubMed  Google Scholar 

  36. N. T.Manojlovic, P. J. Vasiljevic, P. Z. Maskovic, M. Juskovic, G. B. Dusanovic, eCAM. 2012, 1 (2012)

  37. M. Bacˇkorová, R. Jendz ˇelovsky´, M. Kello, M. Bac ˇkor, J. Mikeš, P. Fedorocˇko, Toxicol. In Vitro 26, 462 (2012)

  38. A. Itharat, P.J. Houghton, E. Eno-Amooquaye, P.J Burke, J.H. Sampson, A. Raman, J. Ethnopharmacology 90, 33 (2004)

  39. S.M.K. Swamy, B.K.H. Tan, J. Ethnopharm. 70, 1 (2000)

    Article  CAS  Google Scholar 

  40. B. Ranković, M. Kosanić, T. Stanojković, P. Vasiljević, N. Manojlović, Int. J. Mol. Sci. 13, 14707 (2012)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We would like to express our thanks to the Institute of Biotechnology and Environment, Tay Nguyen University; Department of Genetics, Faculty of Biology and Biotechnology, University of Science, Vietnam National University, Ho Chi Minh City; and Korean Lichen Research Institute, Sunchon National University, Korea. This work was supported in part by a grant from the Ministry of Science and Technology, Taiwan (MOST 102-2313-B-032-001-MY3).

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Authors and Affiliations

  1. Department of Experimental Biology, Faculty of Natural Science and Technology, Tay Nguyen University, Buon Ma Thuot City, Vietnam

    Thi Thu Hoai Nguyen

  2. Hi-tech Agricultural Park, Ho Chi Minh City, Vietnam

    Minh Hiep Dinh

  3. Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City, Vietnam

    Hoang Thanh Chi

  4. Life Science Center/Department of Chemistry, Tamkang University, New Taipei City, Taiwan

    San-Lang Wang

  5. Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot City, Vietnam

    QuangVinh Nguyen, Trung Dzung Tran & Anh Dzung Nguyen

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  1. Thi Thu Hoai Nguyen
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Correspondence to San-Lang Wang or Anh Dzung Nguyen.

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Nguyen, T.T.H., Dinh, M.H., Chi, H.T. et al. Antioxidant and cytotoxic activity of lichens collected from Bidoup Nui Ba National Park, Vietnam. Res Chem Intermed 45, 33–49 (2019). https://doi.org/10.1007/s11164-018-3628-1

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  • DOI: https://doi.org/10.1007/s11164-018-3628-1

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