Skip to main content
SpringerLink
Log in

Phylogenetic analysis of rDNA sequences indicates that the sequestrate Amogaster viridiglebus is derived from within the agaricoid genus Lepiota (Agaricaceae)

  • Short Communication
  • Published:
Mycological Progress Aims and scope Submit manuscript

Abstract

The rare sequestrate fungus Amogaster viridiglebus is known from only one collection in California where it was discovered among Populus roots. Based on sporocarp coloration and spore morphology, this sequestrate taxon was putatively considered to be an ectomycorrhizal member of the Boletales. However, no molecular data were previously available to definitively determine the closest relatives of this fungus. Here we revisit the morphology of Amogaster viridiglebus and present a phylogenetic analysis based on ITS and 28S ribosomal DNA. Our phylogeny indicates that Amogaster viridiglebus is nested in the genus Lepiota, suggesting that this rare species has a saprobic trophic mode and does not form ectomycorrhizae with plants. A new combination, L. viridigleba, is made based on these phylogenetic results.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215(3):403–410. doi:10.1006/jmbi.1990.9999

    PubMed  CAS  Google Scholar 

  • Birkebak JM, Vellinga EC, Franco-Molano AE, Wood MG, Matheny PB (2011) Lepiota maculans, an unusual mushroom rediscovered after 105 years. Southeast Nat 10(2):267–274. doi:10.1656/058.010.0207

    Article  Google Scholar 

  • Castellano MA (1995) NATS truffle and truffle-like fungi.4: Amogaster viridigleba gen. et sp. nov., a new truffle-like fungus from the Sierra Nevada. Mycotaxon 55:185–188

    Google Scholar 

  • Felsenstein J (1985) Confidence-limits on phylogenies—an approach using the bootstrap. Evolution 39(4):783–791. doi:10.2307/2408678

    Article  Google Scholar 

  • Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Mol Ecol 2(2):113–118. doi:10.1111/j.1365-294X.1993.tb00005.x

    Article  PubMed  CAS  Google Scholar 

  • Kropp BR, Albee-Scott S, Castellano MA, Trappe JM (2012) Cryptolepiota, a new sequestrate genus in the Agaricaceae with evidence for adaptive radiation in western North America. Mycologia 104(1):164–174. doi:10.3852/11-046

    Article  PubMed  Google Scholar 

  • Lebel T, Syme A (2012) Sequestrate species of Agaricus and Macrolepiota from Australia: new species and combinations and their position in a calibrated phylogeny. Mycologia 104(2):496–520. doi:10.3852/11-092

    Article  PubMed  Google Scholar 

  • Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University

  • Padovan F, Contu M (2001) Notholepiota sardoa sp. nov., un nuovo gasteromicete filogeneticamente correlato alle Lepiotaceae scoperto in Sardegna. Boll del Gruppo Micologico ‘G Bresadola’, n Ser 44(2):31–36

    Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19(12):1572–1574. doi:10.1093/bioinformatics/btg180

    Article  PubMed  CAS  Google Scholar 

  • Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web servers. Syst Biol 57(5):758–771. doi:10.1080/10635150802429642

    Article  PubMed  Google Scholar 

  • Swofford DL (2003) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland

    Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739. doi:10.1093/molbev/msr121

    Article  PubMed  CAS  Google Scholar 

  • Tedersoo L, May TW, Smith ME (2010) Ectomycorrhizal lifestyle in fungi: global diversity, distribution, and evolution of phylogenetic lineages. Mycorrhiza 20(4):217–263. doi:10.1007/s00572-009-0274-x

    Article  PubMed  Google Scholar 

  • Trappe JM, Molina R, Luoma DL, Cazares E, Pilz D, Smith JE, Castellano MA, Miller SL, Trappe MJ (2009) Diversity, ecology, and conservation of truffle fungi in forests of the Pacific Northwest. U S Forest Service General Technical Report PNW-GTR-772. FS United States Department of Agriculture, Pacific Northwest Research Station (eds) OR: USA

  • Vellinga EC (2002) New combinations in Chlorophyllum. Mycotaxon 83:415–417

    Google Scholar 

  • Vellinga EC (2003) Phylogeny of Lepiota (Agaricaceae) – Evidence from nrITS and nrLSU sequences. Mycol Prog 2(4):305–322

    Article  Google Scholar 

  • Vellinga EC, de Kok RPJ, Bruns TD (2003) Phylogeny and taxonomy of Macrolepiota (Agaricaceae). Mycologia 95(3):442–456. doi:10.2307/3761886

    Article  PubMed  CAS  Google Scholar 

  • Vila J, Castellón E (2003) Some remarks on Notholepiota sardoa Padovan et Contu. Cryptogam Mycol 24(4):303–307

    Google Scholar 

  • White TJ, Bruns TD, Lee S, Taylor J (1990) Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, San Diego, pp 315–322

    Google Scholar 

Download references

Acknowledgments

We appreciate the discussion and comments from Dr. M.A. Castellano who declined co-authorship because he was uncomfortable assigning this species to Lepiota. Financial support for this work came from the University of Florida’s Institute for Food and Agricultural Sciences (IFAS). The authors would like to thank two anonymous reviewers for their helpful comments on the manuscript and thank Dr. Bradley Kropp for sending information on DNA sequences of Cryptolepiota. Morphological examination of W.A. Murrill’s specimen FLAS F9647 of Lepiota clypeolaria was made possible through support from the Florida Museum of Natural History and the Fungal Herbarium of the University of Florida (FLAS).

Author information

Authors and Affiliations

  1. Department of Plant Pathology, University of Florida, Gainesville, FL, USA

    Zai-Wei Ge & Matthew E. Smith

  2. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China

    Zai-Wei Ge

Authors
  1. Zai-Wei Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Matthew E. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Matthew E. Smith.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Table 1

Species information and GenBank accession numbers for sequences used in the phylogenetic analysis. (DOC 59 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ge, ZW., Smith, M.E. Phylogenetic analysis of rDNA sequences indicates that the sequestrate Amogaster viridiglebus is derived from within the agaricoid genus Lepiota (Agaricaceae). Mycol Progress 12, 151–155 (2013). https://doi.org/10.1007/s11557-012-0841-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11557-012-0841-y

Keywords

Search

Navigation