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A new species of Lophodermium on needles of mountain pine (Pinus mugo) from the Giant Mountains in Poland

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Abstract

A fungus tentatively identified as Lophodermium sp. was repeatedly isolated from living secondary needles of mountain pine (Pinus mugo) in the Giant Mountains of Poland. Needles showed symptoms of yellow spots, gradual discolouration and premature falling in July. Thin black zone lines, subepidermal conidiomata and partially subepidermal ascomata morphologically similar to L. pinastri occurred on fallen needles in litter below the same trees. Evidence from internal transcribed spacers of rDNA and the gene for actin showed that strains isolated from symptom-bearing needles and fruiting structures were identical, and differed from other Lophodermium species known from pine, including L. pinastri. The fungus differs subtly from L. pinastri, for example, in the lengths of its conidiomata, ascomata and asci, but can be reliably distinguished only by molecular data. Known only on mountain pine from the Giant Mountains, it is described here as L. corconticum sp. nov.

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References

  • Altschul S, Gish W, Miller W, Myers E, Lipman D (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  PubMed  Google Scholar 

  • Andjelič M (2000) Disease of the mountain pine (Pinus mugo Turra) in the area of “Lovcen” National Park (Montenegro, Yugoslavia). Zaštita Prirode 52:69–78

    Google Scholar 

  • Baudyš E (1924–1925) Příspěvek k rozšíření mikromycetů u nás [Contribution to knowledge of distribution of our micromycetes]. Čas Morav Mus Zem, Brno 22–23:1–31 (In Czech)

  • Burnett JH (1983) Presidential address. Speciation in fungi. Trans Br Mycol Soc 81:1–14

    Article  Google Scholar 

  • Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

    Article  CAS  Google Scholar 

  • Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552

    Article  CAS  PubMed  Google Scholar 

  • Cypers V (1896) Beiträge zur Kryptogamenflora des Riesengebirges und seiner Vorlagen. Pilze. II. Verh Zool-Bot Ver Wien 46:310–320

    Google Scholar 

  • Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nat Meth 9:772

    Article  CAS  Google Scholar 

  • Fassatiová O (1986) Moulds and filamentous fungi in technical microbiology. Elsevier, Amsterdam

    Google Scholar 

  • Ganley RJ, Brunsfeld SJ, Newcombe G (2004) A community of unknown, endophytic fungi in western white pine. Proc Natl Acad Sci U S A 101:10107–10112

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Gao X-M, Lin Y-R, Huang H-Y, Hou C-L (2013) A new species of Lophodermium associated with the needle cast of Cathay silver fir. Mycol Progr 12:141–149

    Article  Google Scholar 

  • Hou C-L, Li L, Piepenbring M (2009) Lophodermium pini-mugonis sp. nov. on needles of Pinus mugo from the Alps based on morphological and molecular data. Mycol Progr 8:29–33

    Article  Google Scholar 

  • Jones SG (1935) The structure of Lophodermium pinastri (Schrad.) Chev. Ann Bot 49:699–728

    Google Scholar 

  • Koukol O, Baldrian P (2012) Intergeneric variability in enzyme production of microfungi from pine litter. Soil Biol Biochem 49:1–3

    Article  CAS  Google Scholar 

  • Luo J-T, Lin Y-R, Shi G-K, Hou C-L (2010) Lophodermium on needles of conifers from Yunnan Province, China. Mycol Progr 9:235–244

  • Lutyk P (1978) The healthiness of mountain pine (Pinus mughus Scop.) in Tatra National Park. Sylwan 122:51–57

    Google Scholar 

  • Millberg H, Boberg JB, Stenlid J (2013) Fungal community composition of Scots pine needles – geographic patterns and variation with needle age and between healthy and diseased needles. IUFRO working party 7.02.02, Brno, Czech Republic, 20–25 May 2013, Book of Abstracts, p 34

  • Miller M, Holder M, Vos R, Midford P, Liebowitz T et al (2009) The CIPRES Portals. CIPRES. 2009-08-04. URL:http://www.phylo. org/sub_sections/portal. Accessed: 2014-01-17. (Archived by WebCite(r) at http://www.webcitation.org/5imQlJeQa)

  • Minter DW (1977) Lophodermium on Pines with Special Reference to Species Occurring on Pinus sylvestris in North-East Scotland. PhD Thesis. 401 pp. UK, Aberdeen; University of Aberdeen, Department of Forestry

  • Minter DW (1981a) Lophodermium on pines. Mycol Pap 147:1–54

    Google Scholar 

  • Minter DW (1981b) Microfungi on needles, twigs and cones of pinus in ČSSR. Čes Mykol 35:90–101

    Google Scholar 

  • Minter DW (1986) Some members of the Rhytismataceae (Ascomycetes) on conifer needles from Central and North America. In: Petersen GW (ed) Recent Research on Conifer Needle Diseases. Proceedings of the IUFRO S2.06.04 Working Party Conference on Needle Diseases, Gulfport, Mississippi, October 14–18, 1984. USDA Forest Service General Technical Report GTR-WO, vol 50. US Department of Agriculture Forest Service, Washington, DC, pp 71–106

    Google Scholar 

  • Minter DW, Holubová-Jechová V (1981) New or interesting Hyphomycetes on decaying pine litter from Czechoslovakia. Folia Geobot Phytotax 16:195–217

    Google Scholar 

  • Minter DW, Millar CS (1980) Ecology and biology of three Lophodermium species on secondary needles of Pinus sylvestris. Eur J For Pathol 10(2–3):169–180

    Article  Google Scholar 

  • Minter DW, Staley JM, Millar CS (1978) Four species of Lophodermium on Pinus sylvestris. Trans Br Mycol Soc 71:295–301

    Article  Google Scholar 

  • Mitchell CP, Millar CS (1978) Mycofloral successions on Corsican pine needles colonized on the tree by three different fungi. Trans Br Mycol Soc 71:303–317

    Article  Google Scholar 

  • Mougeot JB, Nestler CG (1810) Stirpes Criptogamae Vogeso-Rhenanae etc. Fasc 1

  • O’Donnell K (1993) Fusarium and its near relatives. In: Reynolds DR, Taylor JW (eds) The fungal holomorph: mitotic, meiotis and pleomorphic speciation in fungal systematics. CAB International, Wallingford, pp 225–233

    Google Scholar 

  • Osono T, Hirose D (2011) Colonization and lignin decomposition of pine needle litter by Lophodermium pinastri. For Pathol 41:156–162

    Article  Google Scholar 

  • Příhoda A (1965) Houby na kosodřevině v Krkonoších. [Fungi on mountain pine in Giant Mts.]. Oper Corcont 2:61–70, In Czech

    Google Scholar 

  • Przewoźnik L (2008) Plants of Karkonosze National Park. Karkonosze National Park Press, Jelenia Góra (in Polish)

    Google Scholar 

  • Pusz W, Kita W, Kaczmarek A, Nowosad K, Koukol O (2013) The mountain pine’s needles diseases (Pinus mugo) on subalpine zone of Karkonosze Mts. Sylwan 157:761–769

    Google Scholar 

  • Reignoux SNA, Green S, Ennos RA (2014) Molecular identification and relative abundance of cryptic Lophodermium species in natural populations of Scots pine, Pinus sylvestris L. Fungal Biol 118:835–845

    Article  CAS  PubMed  Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  CAS  PubMed  Google Scholar 

  • Schröter J (1908) Die Pilze Schlesiens. - In: Krypt.-Fl. Schlesien, 3–2:1–597

  • Sokolski S, Piche Y, Berube J (2004) Lophodermium macci sp. nov., a new species on senesced foliage of five-needle pines. Mycologia 96:1261–1267

    Article  CAS  PubMed  Google Scholar 

  • Staley JM (1975) The taxonomy of lophodermia on pines with special reference to problems in North American christmas tree plantations. Mitt Bundesforschungsanstalt Forst Holtzwirtschaft 108:79–85

    Google Scholar 

  • Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web-servers. Syst Biol 75:758–771

    Article  Google Scholar 

  • Terrier CA (1942) Essai sur la systématique des Phacidiaceae (Fr.) sensu Nannfeldt (1932). Beitr Kryptogamenflora Schweiz 9:1–99

    Google Scholar 

  • Wang S-J, Lin Y-R, Hou C-L, Gao X-M, Yang Z-Z, Liu F (2010) A preliminary study on phylogeny of Lophodermium based on rDNA-ITS sequences. Mycosystema 29:199–205

    CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols: a guide to methods and applications. Academic, San Diego, pp 315–322

    Chapter  Google Scholar 

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Acknowledgments

The project was supported by Institutional Support for Science and Research of the Ministry of Education, Youth and Sports of the Czech Republic and by the National Science Centre of Poland (grant number N304/069940). We wish to thank Agata Kaczmarek from the Division of Phytopathology and Mycology of the Plant Protection Department at WUELS for her help in preparation of the mycological analyses, and to Wlodzimierz Kita from the Division of Phytopathology and Mycology of the Plant Protection Department at WUELS for his help in collection of needle samples.

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, Charles University in Prague, Benátská 2, CZ-128 44, Prague, Czech Republic

    Ondřej Koukol

  2. Department of Plant Protection, Division of Plant Pathology and Mycology, The Wroclaw University of Environmental and Life Sciences, Grunwaldzki Sq. 24a, Wroclaw, 50-363, Poland

    Wojciech Pusz

  3. CAB International, Bakeham Lane, Egham, Surrey, TW20 9TY, UK

    David Minter

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  1. Ondřej Koukol
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  2. Wojciech Pusz
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  3. David Minter
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Correspondence to Ondřej Koukol.

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Koukol, O., Pusz, W. & Minter, D. A new species of Lophodermium on needles of mountain pine (Pinus mugo) from the Giant Mountains in Poland. Mycol Progress 14, 23 (2015). https://doi.org/10.1007/s11557-015-1038-y

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