Abstract
Based on traditional morphology, sequence data, and phylogenetic analyses, 18 new species are here described: Coprinellus christianopolitanus, Coprinopsis musae, C. udicola, Psathyrella arenosa, P. carminei, P. fennoscandica, P. ichnusae, P. lilliputana, P. lyckebodensis, P. madida, P. rybergii, P. sabuletorum, P. scanica, P. siccophila, P. stridvallii, P. sublatispora, P. vesterholtii, and Typhrasa nanispora. Psathyrella hololanigera and P. tenera are reported as new to Europe and P. parva as new to the Nordic countries. A four-gene dataset on Psathyrellaceae were analyzed by Maximum Parsimony, Maximum Likelihood, and Bayesian methods. Constraint analyses were performed to determine limits of /Psathyrella, and to evaluate whether the /Coprinellus, /cordisporus, and /gossypina clades could be regarded with confidence as monophyletic clades outside of the clade /Psathyrella. This was not unambiguously supported. Based on the phylogenetic results, Kauffmania is proposed as a monotypic genus for the species P. larga and Typhrasa for P. gossypina and the new described species T. nanispora. The genus Homophron is formally validated and three combinations are proposed: H. spadiceum, H. cernuum, and H. camptopodum. The genus Cystoagaricus Singer is emended and the following new combinations are proposed: C. hirtosquamulosus, C. squarrosiceps, C. olivaceogriseus, and C. silvestris. Neotypes have been selected for seven species described by Fries, and ITS sequence data for these were generated. The following new combinations are proposed: Coprinopsis canoceps, C. cineraria, C. melanthina, C. submicrospora, C. uliginicola, and Typhrasa gossypina. Brief comments are given to other species of interest. Psathyrella ornatispora were found not to belong in Psathyrellaceae. A key to 106 psathyrelloid species in Northern Europe is provided.
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References
Arnolds E (2003) Rare and interesting species of Psathyrella. Fungi Non Delineati 26:1–76
Bergsten J, Nilsson AN, Ronquist F (2013) Bayesian tests of topology hypotheses with an example from diving beetles. Syst Biol 62:660–673
Bi Z, Li T, Zheng G (1987) A preliminary study on the genus Psathyrella from Guangdong province of China. Guihaia 7:23–27
Bogart van de F (1975) The Genus Coprinus in Washington and Adjacent Western States. Dissertation. University of Washington
Britzelmayr M (1883) Dermini und Melanospori aus Südbayern. Bericht des Naturhistorischen Vereins Augsburg 27:147–196
Clémençon H, Emmett V, Emmett EE (2004) Cytology and plectology of the hymenomycetes. Bibl Mycol 199:1–488
Cooke WB (1953) The genera of the Homobasidiomycetes. Spec Publ Div Myc Dis Surv US Department of Agriculture 3: 100pp
Donk MA (1962) The generic names proposed for Agaricaceae. Beihefte zur Nova Hedwigia 5:1–320
Galland MC (1972) Caractères mycéliens des Psathyrella (Fr.) Quél. (Agaricales). Rev de Mycologie 36:141–174
Gams W (2002) Report of the committee for fungi: 10. Taxon 51:791–792
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizas and rusts. Mol Ecol 2:113–118
Grgurinovic C (1997) Larger Fungi of South Australia. Botanic Gardens of Adelaide and State Herbarium
Hoashi Y (2008) Psathyrella turcosomarginata, a new species with cheilocystidia possessing mucoid deposits staining bluish green in ammonia solution. Mycoscience 49:385–387
Hopple JS Jr, Vilgalys R (1999) Phylogenetic relationships in the mushroom genus Coprinus and dark spored allies based on sequence data from the nuclear gene coding for the large ribosomal subunit RNA: divergent domains, outgroups, and monophyly. Mol Phylogenet Evol 13:1–19
Horak E (1968) Synopsis generum Agaricalium (Die Gattungstypen der Agaricales). Beiträge zur Kryptogamenflora der Schweiz 13:1–741
Horak E (1980) Taxonomy and distribution of two little known, monotypic genera of Agaricales: Amparoina, Cystoagaricus. Sydowia 33:64–70
Jurand M, Kemp RFO (1972) Surface ultrastructure of Oidia in the Basidiomycete Psathyrella coprophila. J Gen Microbiol 72:575–579
Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. doi:10.1093/molbev/mst010, Online January 16
Keirle MR, Hemmes DE, Desjardin DE (2004) Agaricales of the Hawaiian Islands. 8. Agaricaceae:Coprinus and Podaxis; Psathyrellaceae: Coprinopsis, Coprinellus and Parasola. Fungal Divers 15:33–124
Keizer PJ (1993) Ecology of Macromycetes in roadside verges planted with trees. CIP -Gegevens Koninklijke Bibliotheek, Den Haag, p 290
Kits van Waveren E (1985) The Dutch, French and British species of Psathyrella. Persoonia Suppl 2:1–300
Larsson E, Jacobsson S (2004) The controversy over Hygrophorus cossus settled using ITS sequence data from 200-year-old type material. Mycol Res 108:781–786
Larsson E, Örstadius L (2008) Fourteen coprophilous species of Psathyrella identified in the Nordic countries using morphology and nuclear rDNA sequence data. Mycol Res 112:1165–1185
Matheny PB, Curtis JM, Hofstetter V, Aime MC, Moncalvo JM, Ge ZW, Yang ZL, Slot JC, Ammirati JF, Baroni TJ, Bougher NL, Hughes KW, Lodge DJ, Kerrigan RW, Seidl MT, Aanen DK, DeNitis M, Daniele GM, Desjardin DE, Kropp BR, Norvell LL, Parker A, Vellinga EC, Vilgalys R, Hibbett DS (2006) Major clades of Agaricales: a multilocus phylogenetic overview. Mycologia 98:982–995
Moncalvo JM, Vilgalys R, Redhead SA, Johnson JE, James TY, Aime CM, Hofsetter V, Verduin SJ, Larsson E, Baroni TJ, Thorn GR, Jacobsson S, Clémencon H, Miller OK (2002) One hundred and seventeen clades of euagarics. Mol Phylogenet Evol 23:357–400
Munsell (1975) Munsell soil color charts. Macbeth, Baltimore
Murrill WA (1945) New Florida fungi. Proc Florida Acad Sci 7:107–127
Nagy LG, Kocsubé S, Papp T, Vágvölgyi C (2009) Phylogeny and character evolution of the coprinoid mushroom genus Parasola as inferred from LSU and ITS nrDNA sequence data. Persoonia 22:28–37
Nagy LG, Urban A, Örstadius L, Papp T, Larsson E, Vágvölgyi C (2010) The evolution of autodigestion in the mushroom family Psathyrellaceae (Agaricales) inferred from Maximum Likelihood and bayesian methods. Mol Phylogenet Evol 57:1037–1048
Nagy LG, Walther G, Házi J, Vágvölgyi C, Papp T (2011) Understanding the evolutionary processes of fungal fruiting bodies: correlated evolution and divergence time in the Psathyrellaceae. Syst Biol 60:303–317
Nagy LG, Házi J, Vágvölgyi C, Papp T (2012) Phylogeny and species delimitation in the genus Coprinellus with specieal emphasis on the haired species. Mycologia 104:254–275
Nagy LG, Desjardin ED, Vágvölgyi C, Kemp R, Papp T (2013a) Phylogenetic analyses of Coprinopsis section Lanatuli and Atramentarii identify multiple species within morphologically defined taxa. Mycologia 105:112–114
Nagy LG, Vágvölgyi C, Papp T (2013b) Morphological characterization of clades of the Psathyrellaceae (Agaricales) inferred from a multigene phylogeny. Mycol Prog 12:505–517
Nylander JAA, Wilgenbusch JC, Warren DL, Swofford DL (2008) AWTY (are we there yet?): a system for graphical exploration of MCMC convergence in Bayesian phylogenetics. Bioinformatics 24:581–583
Örstadius L, Knudsen H (2012) Psathyrella (Fr.) Quél. In: Knudsen H, Vesterholt J (eds) Funga Nordica. Agaricoid, boletoid, cyphelloid and gasteroid genera. Nordsvamp, Copenhagen, pp 692–728
Orton PD (1960) New check-list of British Agarics and Boleti. Part 3: notes on genera and species in the list. Trans British Mycol Soc Suppl 43:159–439
Padamsee M, Matheny PB, Dentinger BT, McLaughlin DJ (2008) The mushroom family Psathyrellaceae: evidence for a large-scale phylogeny of the genus Psathyrella. Mol Phylogenet Evol 46:415–429
Pegler DN (1977) A preliminary agaric flora of East Africa. Kew Bull Addit Ser 6:1–615
Pegler DN (1983) Agaric flora of the Lesser Antilles. Kew Bull Addit Ser 9:1–668
Rambaut A, Drummond AJ (2007) Tracer v1.4, Available from http://beast.bio.ed.ac.uk/Tracer
Redhead SA, Vilgalys R, Moncalvo JM, Johnson J, Hopple JS Jr (2001a) Coprinus Pers. and the disposition of Coprinus species sensu lato. Taxon 50:203–241
Redhead SA, Vilgalys R, Moncalvo JM, Johnson J, Hopple JS Jr (2001b) Proposals to conserve the name Psathyrella (Fr.) Quel. with a conserved type and to reject the name Pselliophora P. Karst. (Basidiomycetes: Psathyrellaceae). Taxon 50:275–278
Romagnesi H (1982) Études complémentaires de quelques espèces de Psathyrella ss. lato (Drosophila Quélet). Bull. Trim de la Soc Mycol de France 98:5–68
Selosse M-A, Martos F, Perry BA, Padamsee M, Roy M, Pailler T (2010) Saprotrophic fungal mycorrhizal symbionts in achlorophyllus orchids. Plant Signal Behav 5:1–5
Singer R (1947) New genera of fungi. III. Mycologia 39:77–89
Singer R (1951) The “Agaricales” (Mushrooms) in modern taxonomy. Lilloa 22:1–830
Singer R (1969) Mycoflora australis. Nova Hedwigia Beihefte 29:1–405
Singer R (1978) Interesting and new species of basidiomycetes from Ecuador II. Nova Hedwigia 19:1–98
Singer R (1986) The Agaricales in modern taxonomy, 4th edn. Koeltz Scientific Books, Koenigstein
Smith AH (1972) The North American species of Psathyrella. Mem N Y Bot Gard 24:1–633
Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690
Swofford DL (2003) PAUP*. Phylogenetic analysis using parsimony (* and other methods). Version 4. Sinauer, Sunderland
Uljé CB (2005) Coprinus. In: Noordeloos ME, Kuyper TW, Vellinga EC (eds) Flora Agaricina Neerlandica 6:22–109. CRC press, Boca Raton
Vašutová M, Antonin V, Urban A (2008) Phylogenetic studies in Psathyrella focusing on section Pennatae and Spadiceae –new evidence for the paraphyly of the genus. Mycol Res 112:1153–1164
Vellinga EC (1988) Glossary. Pp. 54–64. In: Bas C, Kuyper THW, Noordeloos ME, Vellinga EC. Flora Agaricina Neerlandica, vol 1. Critical monographs on families of Agarics and Boleti occurring in the Netherlands. AA Balkema, Rotterdam/Brookfield
Vellinga EC (2004) Genera in the Agaricaceae – evidence from nrITS and nrLSU sequences. Mycol Res 108:354–377
White TJ, Bruns T, Lee L, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sininski JJ, White TJ (eds) PCR protocols, a guide to methods and applications. Academic Press, New York, pp 315–322
Acknowledgments
The authors want to express their gratitude to several contributors of valuable material and for assistance of various kinds: Eef Arnolds, David Boertmann, Manfred Enderle, Guillaume Eyssartier, Tomas Hallingbäck, Sven-Åke Hanson, Anton Hausknecht, Stig Jacobsson, Thomas Laessøe, Karl-Henrik Larsson, Erik Ljungstrand, Erhard Ludwig, Andreas Melzer, Jens H. Petersen, Arne Ryberg, Laszlo Nagy, Svengunnar Ryman. To the curators of the following herbaria: AH, AMNH, B, BAFC, BR, C, CBM, CUP, E, F, G, GDGM, GH, H, K, L, LD, MICH, NY, NYS, O, PC, S, SGO, SZE, TAA, TROM, TURA, UPS, WAG-W, WBS, WU, XAL, ZT. Financial support was received from The Swedish Taxonomy Initiative, ArtDatabanken, SLU, Uppsala (EL)
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Additional specimens morphologically studied (DOC 476 kb)
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A key to the psathyrelloid species in Northern Europe (PDF 228 kb)
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Illustrations of the new described and identified species (PDF 1821 kb)
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Örstadius, L., Ryberg, M. & Larsson, E. Molecular phylogenetics and taxonomy in Psathyrellaceae (Agaricales) with focus on psathyrelloid species: introduction of three new genera and 18 new species. Mycol Progress 14, 25 (2015). https://doi.org/10.1007/s11557-015-1047-x
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DOI: https://doi.org/10.1007/s11557-015-1047-x