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Taxonomic and phylogenetic placement of Nodulosphaeria

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Abstract

Nodulosphaeria is a ubiquitous genus that comprises saprobic, endophytic and pathogenic species associated with a wide variety of substrates and has 64 species epithets listed in Index Fungorum. The classification of species in the genus has been a major challenge due to a lack of understanding of the importance of characters used to distinguish taxa, as well as the lack of reference strains. The present study clarifies the phylogenetic placement of the genus and related species, using fresh collections from Italy. Four Nodulosphaeria species are characterized based on multi-loci analyses of ITS, LSU, SSU, TEF and RPB2 sequence datasets. Phylogenetic analyses indicate that Nodulosphaeria species group within the family Phaeosphaeriaceae as a distinct genus. The sexual morphs of Nodulosphaeria hirta and N. spectabilis are described and illustrated using modern concepts. Two new Nodulosphaeria species are introduced. The phylogenetic relationships and taxonomy of the genus Nodulosphaeria are discussed, but further sampling with fresh collections, reference or ex-type strains and molecular data are needed to obtain a better and natural classification for the genus.

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Acknowledgments

This work was supported by the International Research Group Program (IRG-14-27), Deanship of Scientific Research, King Saud University, Saudi Arabia. Ausana Mapook is grateful to Research and Researchers for Industries (RRI) PHD57I0012 under the Thailand Research Fund for providing financial support. Saranyaphat Boonmee thanks to Thailand Research Fund, project number TRG5880152. K.D. Hyde thanks the Chinese Academy of Sciences, project number 2013T2S0030, for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany. We thanks the Plant Germplasm and Genomics Center in Germplasm Bank of Wild Species, Kunming Institute of Botany for the help with molecular work. Thanks are extended to Chonticha Singtripop, Li Junfu, Dhanushka N. Wanasinghe for their help in obtaining sequence data. Ausana Mapook also thanks Alan J.L. Phillips, Shaun Pennycook, Rungtiwa Phookamsak, Mingkwan Doilom, Saranyaphat Boonmee, J.K. Lui and K.W. Thilini Chethana for their valuable suggestions and help.

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Authors and Affiliations

  1. Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, People’s Republic of China

    Ausana Mapook & K. D. Hyde

  2. World Agroforestry Centre, East and Central Asia, Kunming, 650201, Yunnan, People’s Republic of China

    Ausana Mapook & K. D. Hyde

  3. Botany and Microbiology Department, College of Science, King Saud University, Riyadh, 1145, Saudi Arabia

    E. B. Gareth Jones, Ali H. Bahkali & K. D. Hyde

  4. School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Ausana Mapook, Hiran A. Ariyawansa & Saowaluck Tibpromma

  5. Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Ausana Mapook, Saranyaphat Boonmee, Hiran A. Ariyawansa, Saowaluck Tibpromma & K. D. Hyde

  6. A.M.B. Gruppo Micologico Forlivese “Antonio Cicognani”, Via Roma 18, Forlì, Italy

    Erio Campesori

  7. A.M.B. Circolo Micologico “Giovanni Carini”, C.P. 314, Brescia, Italy

    Erio Campesori

  8. Società per gli Studi Naturalistici della Romagna, C.P. 144, Bagnacavallo, RA, Italy

    Erio Campesori

  9. Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, 550006, Guiyang, Guizhou, People’s Republic of China

    Hiran A. Ariyawansa

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  1. Ausana Mapook
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  2. Saranyaphat Boonmee
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  3. Hiran A. Ariyawansa
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  6. E. B. Gareth Jones
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Correspondence to K. D. Hyde.

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Mapook, A., Boonmee, S., Ariyawansa, H.A. et al. Taxonomic and phylogenetic placement of Nodulosphaeria . Mycol Progress 15, 34 (2016). https://doi.org/10.1007/s11557-016-1176-x

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