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Species diversity of Pseudocercospora from Far East Asia

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Abstract

This study reflects on the monophyly of, and species diversity within, the genus Pseudocercospora in Far East Asia. Morphological characteristics and phylogenetic analyses of Pseudocercospora species were based on type specimens and ex-type cultures, which were collected from Japan and Taiwan. A phylogenetic tree was generated from multi-locus DNA sequence data of the internal transcribed spacer regions of the nrDNA cistron (ITS), partial actin (actA), and partial translation elongation factor 1-alpha (tef1), as well as the partial DNA-directed RNA polymerase II second largest subunit (rpb2). Based on these results, Pseudocercospora amelanchieris on Amelanchier and Ps. iwakiensis on Ilex were newly described from Japan, and a further 22 types (incl. two neo-, five lecto-, and 15 epitypes), were designated. The genus Pseudocercospora as presently circumscribed was found to be monophyletic, while the secondary barcodes, actA, tef1, and rpb2 were shown to be well suited to delimitate species within the genus.

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Acknowledgments

This work was supported by grants to the first author by JSPS KAKENHI (grant #17780122, 24780149), JSPS Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation (S2507), Institute for fermentation, Osaka and Genebank, National Institute of Agrobiological Science, Japan.

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Authors and Affiliations

  1. Graduate school of Bioresources, Mie University, Mie, Japan

    Chiharu Nakashima

  2. Faculty of Regional Environment Science, Tokyo University of Agriculture, Tokyo, Japan

    Keiichi Motohashi

  3. Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan

    Chi-Yu Chen

  4. CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands

    Johannes Z. Groenewald & Pedro W. Crous

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  1. Chiharu Nakashima
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  2. Keiichi Motohashi
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  3. Chi-Yu Chen
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  4. Johannes Z. Groenewald
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  5. Pedro W. Crous
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Correspondence to Chiharu Nakashima.

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Section Editor: Roland Kirschner and Pedro W. Crous

This article is part of the Special Issue “Biodiversity of Hyphomycetes - Special Issue in honor of Dr. Subramanian.

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Nakashima, C., Motohashi, K., Chen, CY. et al. Species diversity of Pseudocercospora from Far East Asia. Mycol Progress 15, 1093–1117 (2016). https://doi.org/10.1007/s11557-016-1231-7

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