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Phylogeny of three Ramularia species occurring on medicinal plants of the Lamiaceae

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Abstract

The plant family Lamiaceae is known for its vast quantity of species and genera with many qualities of medicinal, culinary, and agricultural importance. During a survey exploring ramularia-like taxa associated with leaf spot diseases in northern Iran, several Ramularia isolates were found on symptomatic leaves on different medicinal plants of the Lamiaceae. These isolates were studied by applying a polyphasic approach including morphological and cultural data, and multigene phylogeny (ITS, ACT, TEF1-α, GAPDH, and RPB2). The isolates were grouped in three phylogenetically well-supported newly discovered clades. Of these, R. lamiigena on Lamium album is described as a new species. Furthermore, R. menthae on Mentha arvensis and R. stachydis on Stachys annua, which have previously been reduced to synonymy under R. lamii var. lamii based on morphological data, are reinstated herein based on molecular data. All three species are illustrated, and their morphology and phylogenetic relationships to other Ramularia species are discussed.

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Data availability

All sequence data generated in this study (Table 1) are available at GenBank (https://www.ncbi.nlm.nih.gov/genbank/). Alignments can be accessed via TreeBase (http://www.treebase.org).

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Acknowledgments

The authors thank Prof. Dr. Uwe Braun (Martin Luther University, Halle, Germany) for his helpful advice concerning the morphological identification of the species and his beneficial comments on the manuscript. We gratefully acknowledge the Research Deputy of the Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), for financial support.

Funding

This research was financially supported by Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO).

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Authors and Affiliations

  1. Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 19395-1454, Tehran, Iran

    Mounes Bakhshi, Rasoul Zare & Hossein Jafary

  2. Plant Protection Department, Faculty of Agriculture, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran

    Mahdi Arzanlou

  3. Plant Protection Research Department, Golestan Agricultural and Natural Resources Research and Education Center, AREEO, Gorgan, Iran

    Hojjatollah Rabbani nasab

Authors
  1. Mounes Bakhshi
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  2. Rasoul Zare
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  3. Hossein Jafary
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  4. Mahdi Arzanlou
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  5. Hojjatollah Rabbani nasab
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Contributions

Samples were collected by Mounes Bakhshi, Mahdi Arzanlou and Hojjatollah Rabbani nasab. Experiments, morphological and molecular analyses were conducted by Mounes Bakhshi. Rasoul Zare and Hossein Jafary contributed to the data analyses of the new taxon and funding acquisition. The first draft of the manuscript was written by Mounes Bakhshi, and all authors commented on previous versions of the manuscript. Mounes Bakhshi and Rasoul Zare read and approved the final manuscript. All authors have agreed to the published version of the manuscript.

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Correspondence to Rasoul Zare.

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Bakhshi, M., Zare, R., Jafary, H. et al. Phylogeny of three Ramularia species occurring on medicinal plants of the Lamiaceae. Mycol Progress 20, 27–38 (2021). https://doi.org/10.1007/s11557-020-01653-w

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  • DOI: https://doi.org/10.1007/s11557-020-01653-w

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