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Polyphasic taxonomy of four passalora-like taxa occurring on fruit and forest trees

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Abstract

Species of Passalora s. lat. are phytopathogenic fungi that generally cause leaf spot diseases on a broad variety of plants throughout the world. During our investigations exploring cercosporoid fungi associated with leaf spot symptoms of fruit and forest trees in northern and north-western Iran, several passalora-like fungi were isolated from symptomatic leaves of trees belonging to the Fabaceae, Malvaceae, Rosaceae, and Ulmaceae. A polyphasic taxonomic approach applying molecular data, morphological features, and host data was employed to identify the isolates. In a multi-gene phylogenetic analysis (LSU, ITS, and RPB2), these isolates are clustered in four clades in the Mycosphaerellaceae. The taxa encompassed Paracercosporidium microsorum on Tilia platyphyllos, Prathigadoides gleditsiae-caspicae gen. et. sp. nov. on Gleditsia caspica, Pruniphilomyces circumscissus on Prunus avium and Prunus cerasus, and Sirosporium celtidis on Celtis australis. The new genus Prathigadoides and its type species Prathigadoides gleditsiae-caspicae are molecularly distinct from all phylogenetically related genera, and some characteristics of the conidiophores and conidia differ from those of the morphologically similar species Prathigada condensata on the North America Gleditsia triacanthos.

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Availability of data and material

All sequence data generated in this study (Table 1) are available at GenBank (https://www.ncbi.nlm.nih.gov/genbank/). Alignments can be accessed via TreeBase (http://www.treebase.org).

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Acknowledgements

We acknowledge the Research Deputy of the Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran and Horticultural Science Research Institute, Citrus and Subtropical Fruits Research Center, Ramsar, Iran, for financial support.

Funding

This research was financially supported by Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO).

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Authors and Affiliations

  1. Agricultural Research, Education and Extension Organization (AREEO), Iranian Research Institute of Plant Protection, P.O. Box 19395-1454, Tehran, Iran

    Mounes Bakhshi & Rasoul Zare

  2. Institut für Biologie, Bereich Geobotanik Und Botanischer Garten, Martin-Luther-Universität, Herbarium, Neuwerk 21, 06099, Halle, Saale, Germany

    Uwe Braun

  3. Agricultural Research Education and Extension Organization (AREEO), Horticultural Science Research Institute, Citrus and Subtropical Fruits Research Center, P.O. Box 46915-335, Ramsar, Iran

    Hossein Taheri

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  1. Mounes Bakhshi
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Contributions

Samples were collected by Mounes Bakhshi and Hossein Taheri. Experiments and morphological and molecular analyses were conducted by Mounes Bakhshi. Rasoul Zare contributed to the data analyses of the taxa and funding acquisition. Uwe Braun contributed to the morphological analyses of the taxa. The first draft of the manuscript was written by Mounes Bakhshi, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript and have agreed to the published version of the manuscript.

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Correspondence to Mounes Bakhshi.

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Bakhshi, M., Zare, R., Braun, U. et al. Polyphasic taxonomy of four passalora-like taxa occurring on fruit and forest trees. Mycol Progress 20, 1157–1173 (2021). https://doi.org/10.1007/s11557-021-01725-5

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