Abstract
The genus Calonectria accommodates many important pathogens of agricultural, horticultural and forestry crops, including Eucalyptus. During 2017 surveys of Eucalyptus diseases in Sabah, Malaysia, typical symptoms of Calonectria leaf blight were observed. A large number of Calonectria isolates were collected from diseased leaves and soils associated with symptomatic trees. The aim of this study was to identify and resolve the phylogenetic relationships between these isolates using morphological characters and DNA sequence comparisons for six gene regions. From a collection of 73 isolates, eight species residing in three species complexes were identified. Amongst these, four undescribed species were characterized and are named here as Ca. borneana, Ca. ladang, Ca. pseudomalesiana and Ca. tanah. Results of this study support the view that planted Eucalyptus in tropical and subtropical areas of the world represent niches remarkably rich in Calonectria spp. This also has implications for the management of diseases on these important trees.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We thank Mohd Redzuan Bin Abdul Rauf for helping with the surveys and fungal isolations. WenXia Wu and QianLi Liu assisted with data analyses for which we are most grateful.
Funding
We acknowledge the financial support of the Borneo Forestry Co-operative, the Tree Protection Co-operative Programme (TPCP) and the DST/NRF Centre of Excellence in Plant Health Biotechnology (CPHB), South Africa.
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Nam Q. Pham and Michael J. Wingfield contributed to the study conception and design. Michael J. Wingfield collected the samples. Nam Q. Pham and Seonju Marincowitz performed the laboratory work and data analysis. The first draft of the manuscript was written by Nam Q. Pham and all authors contributed to subsequent versions. All authors read and approved the final manuscript.
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Pham, N., Marincowitz, S., Chen, S. et al. Calonectria species, including four novel taxa, associated with Eucalyptus in Malaysia. Mycol Progress 21, 181–197 (2022). https://doi.org/10.1007/s11557-021-01768-8
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DOI: https://doi.org/10.1007/s11557-021-01768-8