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New species of the family Didymellaceae in Iran

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Abstract

The family Didymellaceae is rich in genera and species diversity, host range, and geographic distribution. We describe novel members of the Didymellaceae from various host plants in Iran. Our phylogenetic analyses based on sequence data of the internal transcribed spacer regions 1 and 2 including the intervening 5.8S nuclear ribosomal DNA (ITS), partial nuclear 28S ribosomal DNA (LSU), β-tubulin (TUB2), and RNA polymerase II second largest subunit (RPB2), together with morphological studies supported the introduction of four novel species including Ascochyta amygdali from Amygdalus scoparia, Didymella cylindrica from Pteridium aquilinum, Paramicrosphaeropsis iranica from Quercus brantii, and Xenodidymella menthae from Mentha piperita. The report of all above host plants for the fungal genera, except for Didymella, is new. Ascochyta amygdali can be distinguished from closely related species (A. herbicola) by lacking cylindrical to subcylindrical conidia. Didymella cylindrica differs from the closely related D. subrosea by smaller pycnidia and longer cylindrical conidia. Paramicrosphaeropsis iranica differs from P. ellipsoidea in having smaller, thick-walled pycnidia and shorter conidia. Xenodidymella menthae distinguishes from the closely related X. catariae by smaller aseptate conidia.

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Data availability

New sequences generated in the current study are deposited in NCBI GenBank and new species in MycoBank. Combined alignment used for phylogenetic analysis included in supplementary file.

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Acknowledgements

The authors would like to thank the Iranian Fungal Culture Collection “IRAN” (Iranian Research Institute of Plant Protection, IRIPP) for providing some strains examined in this study. We also thank engineer Alireza Javadi Estahbanati (IRIPP), Dr Masoomeh Ghobad-Nejhad (Iranian Research Organization for Science and Technology, IROST), and Prof Ewald Langer (University of Kassel, Germany) for their foresight in depositing some strains examined in this study in the Iranian Fungal Culture Collection “IRAN”.

Funding

This work was financially supported by grant (SCU.AP99.294) from the Research Council of Shahid Chamran University of Ahvaz.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Khuzestan Province, Iran

    S. Akram Ahmadpour, Mehdi Mehrabi-Koushki & Reza Farokhinejad

  2. Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mehdi Mehrabi-Koushki

  3. Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

    Bita Asgari

Authors
  1. S. Akram Ahmadpour
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  2. Mehdi Mehrabi-Koushki
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  3. Reza Farokhinejad
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Contributions

S. Akram Ahmadpour carried out sample preparation, fungal isolation and purification, morphometric and morphological determination, DNA isolation, PCR amplification, and contribution to describe the novel species. Mehdi Mehrabi-Koushki carried out the design and implementation of the research, DNA and phylogenetic analyses, and the writing of the manuscript. Two other authors, Reza Farokhinejad and Bita Asgari, contributed to the implementation of the research and the revising of the manuscript.

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Correspondence to Mehdi Mehrabi-Koushki.

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Ahmadpour, S.A., Mehrabi-Koushki, M., Farokhinejad, R. et al. New species of the family Didymellaceae in Iran. Mycol Progress 21, 28 (2022). https://doi.org/10.1007/s11557-022-01800-5

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