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A new Penicillium section Citrina species and series from India

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Abstract

This study introduces a new Penicillium species isolated from soil in Yavatmal, India. Penicillium sanjayi sp. nov. is classified in Penicillium section Citrina. The new species is delimited using phenotypic characters and sequences of the nuclear ribosomal internal transcribed spacer (ITS) rDNA regions, partial beta-tubulin (BenA), calmodulin (CaM), and RNA polymerase II second largest subunit (RPB2) region. Phylogenetic analyses consistently resolved the new species in a well-delineated clade with its close relative P. vascosobrinhoanum (originally published as P. vascosobrinhous), distinct from all other series of section Citrina. As a result, we introduce the series Vascosobrinhoana for this unique lineage. Key distinguishing characteristics such as greyish ruby to ruby centre of colonies (obverse) on malt extract agar (MEA), presence of cream colour sclerotia on oatmeal agar (OA), growth rates on standardised media, growth at 30 °C but lack of growth at 37 °C, additional microscopic characters such as solitary or rarely with a subterminal branch other than predominant monoverticillate penicilli, and conspicuously roughened to verruculose conidial ornamentation distinguish the new species P. sanjayi from other monoverticillate section Citrina species.

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Data availability

All sequence data generated for this study can be accessed via GenBank. Taxonomy details are deposited in MycoBank (nomenclature is verified with Curator).

References

  • Aly AH, Debbab A, Clements C, Edrada-Ebel R,Orlikova B, Diederich M, Wray V, Lin W, Proksch P (2011) NF kappa B inhibitors and antitrypanosomal metabolites from endophytic fungus Penicillium sp. isolated from Limoniumtubi florum. Bioorganic & Medicinal Chemistry 19(1):414–421

  • Akaike H (1974) A new look at the statistical model identification. IEEE Transactions on Automatic Control 19:716–723

    Google Scholar 

  • Aljanabi SM, Martinez I (1997) Universal and rapid salt-extraction of high-quality genomic DNA for PCR-based techniques. Nucleic acids Res 25(22):4692–4703

    CAS  PubMed  PubMed Central  Google Scholar 

  • Bamba R, Sumbali G (1999) Some new unrecorded fungal pathogens associated with post-harvest decay of sweet cherry (Prunus avium L.) in North Indian fruit markets. Proceedings of National Academy of Sciences, India Section B 69(2):183–185

    Google Scholar 

  • Barbosa RN, Bezerra JDP, Santos ACDS, Melo RFR, Houbraken J, Oliveira NT, Souza-Motta CMD (2020) Brazilian tropical dry forest (Caatinga) in the spotlight: an overview of species of Aspergillus, Penicillium and Talaromyces (Eurotiales) and the description of P. vascosobrinhous sp. nov. Acta Botanica Brasilica 34(2):409–429

    Google Scholar 

  • van Beyma Thoe Kingma FH (1940) Beschreibung einiger neuer Pilzarten aus dem Centraalbureau voor Schimmelcultures, Baarn (Nederland) VI. Antonie van Leeuwenhoek 6:263–290

    Google Scholar 

  • Bilgrami KS, Jamaluddin S, Rizwi MA (1991) Fungi of India, Edn 2. Today and Tomorrow’s Printers & Publishers, New Delhi, India.1–798.

  • Biourge P (1923) Les moisissures du groupe Penicillium Link. Cellule 33:7–331

    Google Scholar 

  • Chauhan S, Gupta A (1984) Three new rots of Terminalia fruit caused by Penicillium spp. in Pachmarhi hills of Madhya Pradesh. National Academy Sciences, India. Science Letters 7(12):361–363

    Google Scholar 

  • Chauhan S, Gupta AK (1986) Two new soft rot diseases of fruits. Indian Journal of Mycology and Plant Pathology 15(1):68–69

    Google Scholar 

  • Chauhan SK, Tyagi VK, Nagar ML (1980) Mycoflora of soil around pneumatophores of Soneratia acida L. in Andaman Islands. Journal of the Indian Botanical Society 59(4):281–285

    Google Scholar 

  • Devi S, Wadhwani K (1983) Natural association between spores of pteridophytes and fungi. Journal of Environmental Biology 4:1–8

    Google Scholar 

  • Dierckx RP (1901) Un essai de revision du genre Penicillium Link. Annales dela Societe Scientifique Bruxelles 25:83–89

    Google Scholar 

  • Ghosh GR, Dutta BG (1960) Soil fungi from Orissa (India) - I. Mycologia 52(6):915–918

    Google Scholar 

  • Ghosh GR, Dutta BG (1962) Soil fungi from the paddy fields of Orissa. Proceedings of the 49th Indian Science Congress Association Part III: 1–244.

  • Glass NL, Donaldson GC (1995) Development of premier sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61:1323–1330

    CAS  PubMed  PubMed Central  Google Scholar 

  • Hind HG (1940) The constitution of carviolin: Acolouring matter of Penicillium carmino-violaceum Biourge. Biochemical Journal 34(4):577

    CAS  PubMed Central  PubMed  Google Scholar 

  • Hong SB, Cho HS, Shin HD, Frisvad JC, Samson RA (2006) Novel Neosartorya species isolated from soil in Korea. International Journal of Systematic and Evolutionary Microbiology 56(2):477–486

  • Houbraken J, Frisvad JC, Samson RA (2010) Taxonomy of Penicillium citrinum and related species. Fungal Divers 44:117–133

    Google Scholar 

  • Houbraken J, Frisvad JC, Samson RA (2011a) Taxonomy of Penicillium section Citrina. Stud Mycol 70:53–138

    CAS  PubMed  PubMed Central  Google Scholar 

  • Houbraken J, Kocsubé S, Visagie CM, Yilmaz N, Wang XC, Meijer M, Kraak B, Hubka V, Bensch K, Samson RA, Frisvad JC (2020) Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): an overview of families, genera, subgenera, sections, series and species. Stud Mycol 95:5–169

    CAS  PubMed  PubMed Central  Google Scholar 

  • Houbraken J, Lopez-Quintero CA, Frisvad JC, Boekhout T, Theelen B, Franco-Molano AE, Samson RA (2011b) Penicillium araracuarense sp. nov., Penicillium elleniae sp. nov., Penicillium penarojense sp. nov., Penicillium vanderhammenii sp. nov. and Penicillium wotroi sp. nov., isolated from leaf litter. International Journal of Systematic and Evolutionary Microbiology 61(6):1462–1475

    PubMed  Google Scholar 

  • Houbraken J, Samson RA (2011) Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Stud Mycol 70:1–51

    CAS  PubMed  PubMed Central  Google Scholar 

  • Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30(4):772–780

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kornerup A, Wanscher JH (1978) Methuen handbook of colour. Methuen & Co Ltd, Copenhagen.

  • Lanfear R, Frandsen PB, Wright AM, Senfeld T, Calcott B (2017) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Mol Bio and Evol 34(3):772–773

    CAS  Google Scholar 

  • Letunic I, Bork P (2016) Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res 44(W1):W242–W245

    CAS  PubMed  PubMed Central  Google Scholar 

  • Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Mol Bio and Evol 16:1799–1808

    CAS  Google Scholar 

  • Luke P, Devi SU (1979) A new species of Bartalinia. Current Science 48(4):171

    Google Scholar 

  • Manoharachary C, Khalis N (1983) Seed mycoflora of coriander and carum. Indian Botanical Reporter 2(1):90–92

    Google Scholar 

  • Mukerjee KG (1966) Ecological studies on the microorganic population of usar soils. Mycopath et Mycol Applicata 29(3–4):339–349

    Google Scholar 

  • Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Molecular Biology and Evolution 32(1):268–274

    CAS  PubMed  Google Scholar 

  • Phookamsak R, Hyde KD, Jeewon R, Bhat DJ, Jones EBG, Maharachchikumbura SSN, Raspé O, Karunarathna SC, Wanasinghe DN, Hongsanan S (2019) Fungal Diversity Notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Divers 95(1):1–273

    Google Scholar 

  • Pitt JI (1980) The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. Academic, London.:1–634

  • Pitt JI, Hocking AD (2009) Fungi and food spoilage. Springer, London

    Google Scholar 

  • Posternak T (1940) Recherches sur la biochimiedes champignons inférieurs IV. Sur le pigment de Penicillium roseo‐purpureum Dierckx. Helvetica Chimica Acta 23(1):1046–1053

  • Rai B, Pathak KK (1981) Studies on phylloplane microflora of potato in relation to air pollutants. Environmental Pollution A 26(2):153–166

    CAS  Google Scholar 

  • Rai JN, Tewari JP, Mukerjee KG (1969) Mycoflora of mangrove mud. Mycopath et Mycol Applicata 38(1–2):17–31

    Google Scholar 

  • Rai RP (1976) Concerning fungi and actinomycetes of rock incrustations. Current Science 45(10):393

    Google Scholar 

  • Rajeshkumar KC, Bhat DJ, Lad SS, Wijayawardene NN, Singh SK, Pandkar MR, Maurya DK, Ashtekar N, Hyde KD (2018) Morphology and phylogeny of Tamhinispora srinivasanii sp. nov. (Tubeufiaceae) from northern Western Ghats, India. Phytotaxa 346(1):113–120. https://doi.org/10.11646/phytotaxa.346.1.7

    Article  Google Scholar 

  • Rajeshkumar KC, Braun U, Groenewald JZ, Lad SS, Ashtekar N, Fatima S, Anand G (2021) Phylogenetic placement and reassessment of Asperisporium pongamiae as Pedrocrousiella pongamiae gen. et comb. nov. (Mycosphaerellaceae). Fungal Sys and Evol 7:165–176. https://doi.org/10.3114/fuse.2021.07.08

    Article  CAS  Google Scholar 

  • Rajeshkumar KC, Hyde KD, Wijayawardene NN, Madrid H, Lad SS, Boonmee S, Shahnoor F (2019b) Tubeufia sahyadriensis (Tubeufiaceae), a new dictyosporous anamorph from the Western Ghats, India. Phytotaxa 423(3):171–181. https://doi.org/10.11646/phytotaxa.423.3.5

    Article  Google Scholar 

  • Rajeshkumar KC, Sharma R (2013) Tamhinispora a new genus belongs to family Tubeufiaceae from the Western Ghats, India based on morphology and phylogenetic analysis. Mycosphere 4(2):165–175. https://doi.org/10.5943/mycosphere/4/2/2

    Article  Google Scholar 

  • Rajeshkumar KC, Singh SK (2012) Manoharachariella indica sp. nov. from the Western Ghats, India. Mycotaxon 120:43–48. https://doi.org/10.5248/120.43

    Article  Google Scholar 

  • Rajeshkumar KC, Yilmaz N, Marathe SD, Seifert KA (2019a) Morphology and multigene phylogeny of Talaromyces amyrossmaniae, a new synnematous species belonging to the section Trachyspermi from India. MycoKeys 45:41–56

    Google Scholar 

  • Ramirez C (1982) Manual and atlas of the penicillia. Elsevier Biomedical, New York.

  • Ramírez C, Martinez AT (1980) Some new species of Penicillium recovered from the atmosphere in Madrid and from other substrata. Mycopathologia 72(3):181–191

    PubMed  Google Scholar 

  • Raper KB, Thom C (1949) Manual of the penicillia. Williams & Wilkins, Baltimore.

  • Reddy BS, Sehgal HS, Manoharachary C (1982) Studies on seed mycoflora of certain species of Eucalyptus. Acta Bot Indica 10(2):302–303

    Google Scholar 

  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) Mrbayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Sys Biol 61:539–542

    Google Scholar 

  • Roy AN, Sharma G, Sharma RB (1980) Penicillium dry fruit rot of ashgourd. Current Science 49(17):670

    Google Scholar 

  • Roy AN, Sharma RB, Gupta KC (1977) Occurrence of three new rot diseases of stored garlic (Allium sativum L.). Current Science 46(20):716–717

    Google Scholar 

  • Sadhu BP, De AB, Gupta K (1992) A new post-harvest disease of banana. Indian Phytopath 45(4):473

    Google Scholar 

  • Saksena RK, Mehrotra BS (1952) Fungus flora of an Allahabad soil. Proc Nat Aca of Sci of India 22:22–43

    Google Scholar 

  • Samson RA, Houbraken J, Thrane U, Frisvad JC, Andersen B (2010) Food and Indoor Fungi, CBS laboratory manual series 2. CBS-Fungal Biodiversity Centre, Utrecht

    Google Scholar 

  • Saxena AS, Mukerjee KG, Agarwala MK (1969) Spread of fungal spores causing allergic diseases. Aspects of Allergy and Applied Immunology 2:175–180

    Google Scholar 

  • Shang Z, Khalil Z, Li L, Salim AA, Quezada M, Kalansuriya P, Capon RJ (2016) Roseopurpurins: chemical diversity enhanced by convergent biosynthesis and forward and reverse Michael additions. Organicletters 18(17):4340–4343

    CAS  Google Scholar 

  • Stolk AC (1969) Four new species of Penicillium. Antonie van Leeuwenhoek 35:261–274

    CAS  PubMed  Google Scholar 

  • Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM, Hibbett DS, Fisher MC (2000) Phylogenetic species recognition and species concepts in fungi. Fungal genetics and biol 31(1):21–32

    CAS  Google Scholar 

  • Usekar MS (1977) Soil fungi from Maharashtra. Journal of the University of Poona Science and Technology 50:119–126

    Google Scholar 

  • Verma VS, Khan AM (1965) Fungi associated with Sorghum seeds. Mycopath et Mycol Applicata 27(3–4):314–320

    CAS  Google Scholar 

  • Visagie CM, Houbraken J, Frisvad JC, Hong SB, Klaassen CH, Perrone G, Seifert KA, Varga J, Yaguchi T, Samson RA (2014b) Identification and nomenclature of the genus Penicillium. Stud Mycol 78:343–371

    CAS  PubMed  PubMed Central  Google Scholar 

  • Visagie CM, Seifert KA, Houbraken J, Samson RA, Jacobs K (2014a) Diversity of Penicillium section Citrina within the fynbos biome of South Africa, including a new species from a Protea repens infructescence. Mycologia 106(3):537–552

    PubMed  Google Scholar 

  • White TJ, Bruns T, Lee S, Lee SJ, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications (Innis MA, Gelfand DH, Shinsky TJ, White TJ, eds). Academic Press Inc, New York 18(1):315–322.

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Acknowledgements

Kunhiraman C. Rajeshkumar thanks SERB, Department of Science and Technology, Government of India, for financial support under the project CRG/2020/000668. RKC also thanks Dr. P. K. Dhakephalkar, Director, MACS Agharkar Research Institute, Pune, for providing laboratory facilities and motivation in our work. Nikhil Ashtekar thanks CSIR-HRDG, INDIA, for the financial support under SRF fellowship (09/0670(11172)/2021-EMR-I).

Funding

This research was funded by the SERB, DST, India (as mentioned in metadata and Acknowledgements already).

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Authors and Affiliations

  1. National Fungal Culture Collection of India (NFCCI), Biodiversity and Palaeobiology (Fungi) Gr, MACS Agharkar Research Institute, G. G. Agarkar Road, Pune, Maharashtra, 411004, India

    Nikhil Ashtekar & Kunhiraman C. Rajeshkumar

  2. Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa

    Neriman Yilmaz & Cobus M. Visagie

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  1. Nikhil Ashtekar
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  2. Kunhiraman C. Rajeshkumar
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  3. Neriman Yilmaz
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  4. Cobus M. Visagie
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Contributions

Conceptualization: Rajeshkumar KC. Methodology: Rajeshkumar KC, Cobus Visagie, Neriman Yilmaz, Nikhil Ashtekar. Formal analysis and investigation: Cobus Visagie, Neriman Yilmaz (final tree analyses), Rajeshkumar KC, and Nikhil Ashtekar (collection, data, lab work, taxonomy, preliminary analyses). Writing—original draft preparation: Rajeshkumar KC, Cobus Visagie, Nikhil Ashtekar, Neriman Yilmaz. Writing—review and editing: Rajeshkumar KC, Cobus Visagie, Neriman Yilmaz. Funding acquisition: Rajeshkumar KC. Resources: Rajeshkumar KC. Supervision: Rajeshkumar KC, Cobus Visagie.

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Correspondence to Kunhiraman C. Rajeshkumar.

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Ashtekar, N., Rajeshkumar, K.C., Yilmaz, N. et al. A new Penicillium section Citrina species and series from India. Mycol Progress 21, 42 (2022). https://doi.org/10.1007/s11557-022-01802-3

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