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Toxicocladosporium crousianum and T. eucalyptorum, two new foliar fungi associated with Eucalyptus trees

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Abstract

In 2007, Toxicocladosporium was established as new dematiaceous fungal genus in the family Cladosporiaceae that includes numerous plant pathogenic, epiphytic, endophytic, and saprobic fungi. A subsequent investigation on members of leaf-inhabiting fungi in Iran resulted in the identification of two new species belonging to the genus Toxicocladosporium associated with Eucalyptus leaves, described here as T. crousianum and T. eucalyptorum spp. nov. These novel taxa form independent lineages distinct from other taxa, based on morphological and multi-locus phylogenetic analyses. Detailed descriptions and illustrations of these two new taxa are presented. Additionally, the genus Toxicocladosporium represents a new fungal record for Iran.

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Data availability

All sequence data generated in this study (Table 1) are available at GenBank (https://www.ncbi.nlm.nih.gov/genbank/). Alignments used for phylogenetic analysis included in supplementary file.

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Acknowledgements

The authors gratefully acknowledge Prof. Dr. Pedro W. Crous (Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands) for his valuable comments on the manuscript and Prof. Dr. Uwe Braun (Martin-Luther-University, Halle, Germany) for his helpful advice on the nomenclature. We are obliged to the Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), for financial support.

Funding

This research was financially supported by Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO).

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  1. Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 19395-1454, Tehran, Iran

    Mounes Bakhshi, Rasoul Zare & Hossein Jafary

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  1. Mounes Bakhshi
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  2. Rasoul Zare
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Contributions

Experiments, morphological, and molecular analyses were conducted by Mounes Bakhshi. Rasoul Zare and Hossein Jafary contributed to the data analyses of the taxa and funding acquisition. The first draft of the manuscript was written by Mounes Bakhshi and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript and have agreed to the published version of the manuscript.

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Correspondence to Mounes Bakhshi.

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Supplementary information

Supplementary Fig. 1

Consensus phylogram (50% majority rule) resulting from a Bayesian analysis of the LSU sequence alignment using MrBayes 3.2.6. The scale bar indicates 0.05 expected changes per site. The tree was rooted to Neocladosporium leucadendri (CBS 131317) (JPG 642 kb)

Supplementary Fig. 2

Consensus phylogram (50% majority rule) resulting from a Bayesian analysis of the ITS sequence alignment using MrBayes 3.2.6. The scale bar indicates 0.05 expected changes per site. The tree was rooted to Neocladosporium leucadendri (CBS 131317) (JPG 588 kb)

Supplementary Fig. 3

Consensus phylogram (50% majority rule) resulting from a Bayesian analysis of the ACT sequence alignment using MrBayes 3.2.6. The scale bar indicates 0.05 expected changes per site. The tree was rooted to Neocladosporium leucadendri (CBS 131317) (JPG 572 kb)

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Bakhshi, M., Zare, R. & Jafary, H. Toxicocladosporium crousianum and T. eucalyptorum, two new foliar fungi associated with Eucalyptus trees. Mycol Progress 21, 77 (2022). https://doi.org/10.1007/s11557-022-01826-9

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