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The taxonomic revision of Melanogaster (Paxillaceae, Boletales) in China based on molecular and morphological evidence

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Abstract

The sequestrate genus Melanogaster is rich in mainland China. A total of ten taxa are supported by molecular evidence in this study, including five known species, four new species, and one putatively new species (supported by an ITS sequence from ectomycorrhizal root tip). Based on our morphological reexamination of the type or authentic specimens, four species recorded in China, including M. natsii, M. obovatisporus, M. ovoidisporus, and M. ovoidisporus var. angustatisporus, are treated as questionable species. Consequently, four new species, M. minobovatus, M. panzhihuaensis, M. quercicola, and M. tomentellus, are described and illustrated; M. fusisporus var. obovatus is recombined as M. obovatus; both M. spinisporus and M. subglobisporus are epitypified and re-described. A key for Chinese Melanogaster species is provided.

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Data availability

The DNA sequences generated during the current study are available in NCBI Genbank. The alignment used for the phylogenetic analyses has been submitted as Supplementary material.

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Acknowledgements

The authors thank the curators of the herbaria of HMAS for providing specimens and permission of the DNA extraction from them. The authors are grateful to Dr. Jing-zhong Cao and Mr. Jin-song Xu for collecting specimens and providing valuable suggestions. The authors also appreciate the two anonymous reviewers and editors for their suggestions and corrections to improve this paper.

Funding

This study was supported by the National Natural Science Foundation of China (No. 31750001) and the Beijing Natural Science Foundation (No. 5172003).

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  1. Yu-yan Xu and Xiang-yuan Yan contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, Capital Normal University, Beijing, 100048, China

    Yu-yan Xu, Xiang-yuan Yan, Ting Li, Tao-yu Zhao, Jing-chong Lv & Li Fan

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Contributions

All authors contributed to the conception and design of the study. Yu-yan Xu, Xiang-yuan Yan, Ting Li, Tao-yu Zhao, Jing-chong Lv, and Li Fan prepared material. Ting Li, Tao-yu Zhao, and Jing-chong Lv isolated DNA and prepared samples for sequencing. Yu-yan Xu and Xiang-yuan Yan conducted phylogenetic analyses and morphological observations. Yu-yan Xu and Xiang-yuan Yan wrote the first draft of the manuscript, and Yu-yan Xu and Li Fan revised the manuscript. All authors contributed to previous drafts of the manuscript and read and approved the final draft of the manuscript.

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Correspondence to Li Fan.

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Supplementary information

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The alignment of Melanogaster used for the phylogenetic analyses. (FAS 41 kb)

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Xu, Yy., Yan, Xy., Li, T. et al. The taxonomic revision of Melanogaster (Paxillaceae, Boletales) in China based on molecular and morphological evidence. Mycol Progress 21, 98 (2022). https://doi.org/10.1007/s11557-022-01842-9

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