Abstract
Harmoniella junipericola, a new hyphomycete inhabiting on leaf litter of Juniperus chinensis (Cupressaceae) in Japan is described based on morphological characters on natural substrates and in culture and phylogenetic analyses using the sequences of internal transcribed spacer (ITS), large subunit nrRNA gene (LSU; 28S), DNA-directed RNA polymerase I largest subunit gene (RPB1), and DNA-directed RNA polymerase II second largest subunit gene (RPB2). The fungus belongs to the monotypic/monogeneric family Strobiloscyphaceae (one genus with two species; Pezizales) typified by Strobiloscypha. Brief notes are added on morphological and ecological comparisons among H. chrysocephala (type species, in Ukraine), H. campanaensis (in Chile), and H. junipericola. Using the ITS sequences deposited in the DNA Data Bank, additional operational taxonomic units (OTUs) of Harmoniella and phylogenetically related sequences are briefly discussed based on the data from East Asia (Japan, China), Middle East (Israel), Europe (France, Italy, Portugal), and North America (USA).
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Data and material availability
Sequence data are available in the DNA Data Bank (DDBJ/ENA/GenBank; https://www.ddbj.nig.ac.jp/ddbj/index-e.html, https://www.ebi.ac.uk/ena/browser/about, https://www.ncbi.nlm.nih.gov/nuccore) under the accession numbers shown in bold in Table 2. Scientific name of a new species was registered in MycoBank (https://www.mycobank.org/). Specimens and isolates were deposited in herbarium TNS and culture collections JCM and NBRC, respectively, with acronyms explained in the text and Table 2. Alignments used in Figs. 5, 6, and S1 were included in supplementary data.
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Acknowledgments
The authors express our great gratitude to Ms. S. Hayashi, Dr. H. Masuya, Mr. T. Mori, Mr. M. Nanjo, Dr. M. Nitta, Ms. S. Ogawa, Ms. N. Sonoda, Ms. A. Suzuki, Mr. K. Taniuchi, and Mr. T. Watanabe for collecting the samples of J. chinensis and Mr. O. Laurence and Dr. K. Nagai for the biological discussion in the early period. The Support Unit for Bio-Material Analysis, RIKEN CBS Research Resources Division is also thanked for technical help with DNA sequencing of JCM strains. The RIKEN Library supported for getting a high-quality copy of a Russian paper. We gratefully acknowledge Prof. K.A. Seifert, two anonymous reviewers, and Prof. R. Kirschner (Section Editor) for their helpful reviews of the manuscript.
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This work was partially supported by the funds obtained from RIKEN Integrated Symbiology (iSYM) and JSPS KAKENHI (Grant Numbers 19H05689 to M.O. and 21K15155 to A.H.).
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Conceived and designed the experiments: T.M., G.O., A.H., Y.D. Field work, morphological and cultural studies: T.M., G.O., Y.D. Molecular work and phylogenetic analyses: A.H. Writing and reviewing the paper: T.M., G.O., A.H., Y.D. Editing the draft: All authors. The final draft was read and approved by all authors.
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List of new taxon names (new species): Harmoniella junipericola Masaki
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Supplementary file1 (PDF 575 kb) Fig. S1 Neighbor-joining tree of Harmoniella spp. based on ITS1 (A) and ITS2 (B) sequences (cf., Tables 2, 3). NJ BS greater than 50% are presented at the nodes. Each taxon is shown in order “strain/sample name”, “collected country”, and “substrate”. The newly obtained sequences are shown in red. The scale bars represent nucleotide substitutions. “*1” indicates the sequences used only for the ITS1 analysis, and “*2” for the ITS2 analysis. “*3” indicates the sequence registered as “Strobiloscypha cupressina” in GenBank
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Masaki, T., Hashimoto, A., Degawa, Y. et al. Harmoniella junipericola (Strobiloscyphaceae, Pezizales), a new hyphomycete inhabiting on leaf litter of Juniperus chinensis in Japan, its molecular phylogenetic classification, and brief notes on other Harmoniella species. Mycol Progress 22, 36 (2023). https://doi.org/10.1007/s11557-023-01884-7
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DOI: https://doi.org/10.1007/s11557-023-01884-7