Abstract
Octospora tucumanensis, a new bryophilous ascomycete infecting rhizoids of the pleurocarpous moss Dimerodontium balansae (Fabroniaceae), is described and illustrated based on collections from Argentina. The new species has a unique set of morphological characters and is phylogenetically supported. Morphologically, O. tucumanensis is characterized by the globose to subglobose ascospores ornamented with warts of variable size and shape. Differences from other morphologically similar species of Octospora are discussed.
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Data availability
All data generated or analyzed during this study are included in this published article.
All sequence data generated in this study is available on GenBank (see Table 1).
The alignment files and original tree outputs are available in the TreeBase repository http://purl.org/phylo/treebase/phylows/study/TB2:S29688?x-access-code=f8e234db12368538a92a5e94b0b1e13c&format=html).
References
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215(3):403–410
Benkert D (1990) Zwei neue Arten der Gattung Lamprospora (Pezizales, Ascomycetes). Feddes Repert 101:631–637. https://doi.org/10.1002/fedr.19901011112
Benkert D (1998) Beiträge zur Kenntnis bryophiler Pezizales-Arten. 6. Wrightoideae, eine neue Sektion der Gattung Octospora. Zeitschrift Für Mykologie 64:17–40
Calzada A (2021) Octospora itzerottii. Primer registro para la Península Ibérica. Bol Micol FAMCAL 16:9–18
Câmara PE, Magill RE (2009) A review of Dimerodontium (Fabroniaceae). Bryologist 112:301–308. https://doi.org/10.1639/0007-2745-112.2.301
Cash EK (1957) Some Chilean discomycetes collected by Roland Thaxter. Beihefte Zur Sydowia 1:289–293
Dissing H, Sivertsen S (1983) Operculate discomycetes from Rana (Norway) 4. Octospora hygrohypnophila, Peziza prosthetica und Scutellinia mirabilis spp. nov. Nord J Bot 3:415–421
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19:11–15
Eckstein J (2016) Bryoparasitic Pezizales. – http://www.octospora.de (accessed: 25.04.2023)
Eckstein J, Sochorová Z, Janošík L (2021) Octospora oscarii spec. nov. (Pezizales), a bryophilous ascomycete on the pleurocarpous moss Pseudotaxiphyllum elegans (Hypnales). Herzogia 34(2):286–298. https://doi.org/10.13158/heia.34.2.2021.286
Egertová Z, Eckstein J, Sochor M, Vega M (2018) Lamprospora sylvatica (Pyronemataceae), a new bryophilous ascomycete on Dicranum montanum. Phytotaxa 357:17–29. https://doi.org/10.11646/phytotaxa.357.1.2
Egertová Z, Döbbeler P, Sochor M (2018b) Octosporopsis erinacea and Octospora kelabitiana (Pezizales) – two new hepaticolous ascomycetes from Borneo. Mycol Prog 17:103–113. https://doi.org/10.1007/s11557-017-1354-5
Gamundí IJ (1960) Discomycetes operculados de Argentina: Familias Pezizaceae y Humariaceae. Lilloa 30:257–338
Gamundí IJ, Minter DW, Romero AI, Barrerea VA, Giaiotti LA, Messuti MI, Stecconi M (2004) Checklist of the discomycetes (Fungi) of Patagonia, Tierra del Fuego and adjacent Antarctic areas. Darwiniana 42:63–164
Goloboff PA (1999) Analyzing large data sets in reasonable times: solutions for composite optima. Cladistics 15(4):415–428
Goloboff PA, Catalano SA (2016) TNT version 1.5, including a full implementation of phylogenetic morphometrics. Cladistics 32(3):221–238
Guindon S, Dufayard J-F, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59(3):307–321. https://doi.org/10.1093/sysbio/syq010
Hansen K, Olariaga I (2015) Species limits and relationships within Otidea inferred from multiple gene phylogenies. Mol Phylogeny Evol Fungi 35(1):148–165. https://doi.org/10.3767/003158515X687993
Hepperle D (2011) DNA Dragon 1.4. 1-DNA sequence contig assembler software. Available at: https://www.dna-dragon.com. Accessed 8 Aug 2022
Hyde KD, Udayanga D, Manamgoda DS, Tedersoo L, Larsson E, Abarenkov K, Bertrand YJK, Oxelman B, Hartmann M, Kauserud H, Ryberg M, Kristiansson E, Nilsson RH (2013) Incorporating molecular data in fungal systematics: a guide for aspiring researchers. Curr Res Environ Appl Mycol 3:1–32
Kalyaanamoorthy S, Minh BQ, Wong TK, Von Haeseler A, Jermiin LS (2017) ModelFinder: fast model selection for accurate phylogenetic estimates. Nat Methods 14(6):587–589
Letunic I, Bork P (2007) Interactive Tree of Life (iTOL): an online tool for phylogenetic tree display and annotation. Bioinformatics 23(1):127–128. https://doi.org/10.1093/bioinformatics/btl529
Minh BQ, Minh ATN, von Haeseler A (2013) Ultrafast approximation for phylogenetic bootstrap. Mol Biol Evol 30(5):1188–1195. https://doi.org/10.1093/molbev/mst024
Németh C (2017) Octospora erzbergeri (Pyronemataceae), a bryophilous ascomycete in Hungary. Studia Bot Hung 48(1):105–123
Nemeth C, Eckstein J, Sochor M (2022) Disentangling the taxonomy of Octospora meslinii (Pezizales), a bryophilous ascomycete on Grimmia pulvinata. Czech Mycol 74(1):1–24. https://doi.org/10.33585/cmy.74101
Nguyen LT, Schmidt HA, Von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol 32(1):268–274
O’Donnell K, Cigelnik E, Nirenberg HI (1998) Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90:465–493
O’Shea BJ, Price MJ (2008) An updated checklist of the mosses of Paraguay. Bryophyte Divers Evol 29:6–37. https://doi.org/10.11646/bde.29.1.3
Rehner SA, Buckley E (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97(1):84–98. https://doi.org/10.1080/15572536.2006.11832842
Romero AI, Minter DW (1988) Fluorescence microscopy: an aid to the elucidation of ascomycete structures. Trans Br Mycol Soc 90:457–470. https://doi.org/10.1016/S0007-1536(88)80156-6
Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling A, Höhna S et al (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61(3):539–542
Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL et al (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci 109(16):6241–6246. https://doi.org/10.1073/pnas.1117018109
Seaver FJ (1914) A preliminary study of the genus Lamprospora. Mycologia 6(1):5–24
Sochorová Z, Döbbeler P, Sochor M, van Rooy J (2019) Octospora conidiophora (Pyronemataceae) – a new species from South Africa and the first report of anamorph in bryophilous Pezizales. MycoKeys 54:49–76. https://doi.org/10.3897/mycokeys.54.34571
Sochorová Z, Matočec N, Kušan I, Janošík L, Eckstein J, Vega M, Mešić A, Sedlářová M, Martínez-Gil R, Sochor M (2020) Amended description of the rarely reported bryophilous ascomycete Octospora svrcekii (Pyronemataceae) with notes on the phylogeny of the section Wrightoideae. Phytotaxa 475(1):1–17. https://doi.org/10.11646/phytotaxa.475.1.1
Sochorová Z, Eckstein J, Sedlárová M, Sochor M (2021) Octospora doebbeleri, a new bryophilous species on Dicranoweissia cirrata. Sydowia 73:233–244
Spegazzini CL (1918) Cuarta contribución a la micología Chilena. Rev Chil Hist Nat 22(2–3):95–104
Stenroos S, Laukka T, Huhtinen S, Döbbeler P, Myllys L, Syrjänen K, Hyvönen J (2010) Multiple origins of symbioses between ascomycetes and bryophytes suggested by a five-gene phylogeny. Cladistics 26:281–300
Stevenson JA (1975) The fungi of Puerto Rico and the American Virgin Islands. Contrib Reed Herb 23:743
Tamura K, Stecher G, Kumar S (2021) MEGA11: Molecular Evolutionary Genetics Analysis version 11. Mol Biol Evol, MBE 38(7):3022–3027. https://doi.org/10.1093/molbev/msab120
Thiers BM (2023) Index Herbariorum. http://sweetgum.nybg.org/science/ih/. Accessed 16 Apr 2023
Vega M, Eckstein J, Van der Kolk H-J (2016) Lamprospora verrucispora sp. nov. (Pezizales). Ascomycete.org 8(4):163–171. https://doi.org/10.25664/art-0184
Vega M, Janošík L, Sochorová Z, Martínez-Gil R, Eckstein J (2019) Lamprospora densireticulata sp. nov., L dictydiola and L carbonicola (Pyronemataceae, Pezizales) three very similar species from very different hosts and habitats. Mycol Prog 18(8):1013–1026. https://doi.org/10.1007/s11557-019-01505-2
Vega M, Janošík L, Eckstein J, Martínez-Gil R, Rubio E (2021) Warts galore – on three new Lamprospora De Not. Species (Pezizales) from Southern Europe and Macaronesia and a type revision of three species described from the US by F. J. Seaver in the 1910s. Cryptog Mycol 42(6):91–119. https://doi.org/10.5252/cryptogamie-mycologie2021v42a6
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. Academic Press, San Diego, California, A guide to methods and applications, pp 315–322
Xiang C-Y, Gao F, Jakovlić I, Lei H-P, Hu Y, Zhang H, Zou H, Wang GT, Zhang D (2023) Using PhyloSuite for molecular phylogeny and tree-based analyses. Imeta 2(1):1–42. https://doi.org/10.1002/imt2.87
Zhang D, Gao F, Jakovlić I, Zou H, Zhang J, Li WX, Wang GT (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Mol Ecol Resour 20(1):348–355. https://doi.org/10.1111/1755-0998.13096
Acknowledgements
We are highly indebted to Jan Eckstein (Germany) for the comments on O. tucumanensis. We thank the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), PIUNT G744 and Project B-0024-1 (Fundación Miguel Lillo) for the financial support.
Funding
GS, JF: The research was financed by the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and PIUNT G744. MC: Project B-0024–1 (Fundación Miguel Lillo).
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MC, GMS did the morphological analyses and assembled the descriptions; FD, HP and JF did the molecular data and phylogenetic analyses; all authors analyzed the results and wrote the manuscript. All authors read and approved the final manuscript.
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Suárez, G.M., Dominguez, F.G., Pajot, H. et al. Octospora tucumanensis (Pezizales), a new bryophilous ascomycete on Dimerodontium balansae (Bryophyta) from Argentina. Mycol Progress 22, 54 (2023). https://doi.org/10.1007/s11557-023-01909-1
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DOI: https://doi.org/10.1007/s11557-023-01909-1