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Study of a new strain of Sanchytrium tribonematis expands our knowledge on Sanchytriomycota (Fungi)

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Abstract

Recently, phylogenomic analyses of two parasitic fungi with amoeboid zoospores and long kinetosomes, the sanchytrids Amoeboradix gromovi and Sanchytrium tribonematis, showed that they formed a clade Sanchytriomycota sister to Blastocladiomycota. Sanchytrid species diversity is still very low and most of the isolates from different places belong to S. tribonematis. Here, we present a new strain X-137 CCPP ZIN RAS of fresh-water S. tribonematis having a peculiar morphology and an unusual stage in the life cycle. Its zoospores have a kinetosome composed of 9 microtubular singlets along its whole length (1.2 μm) with the pseudocilium containing an axoneme of 4 microtubules and enable to form an extremely long posterior filopodium (up to 10 body lengths). The kinetosome develops from a centriole of the centrosomal apparatus in sporangium, while another centriole becomes a non-flagellar kinetosome. Big zoospores with several pseudocilia and many (up to eleven) kinetosomes per cell have been found in S. tribonematis culture. They are probably a result of several zoospore or gamete fusions. These findings expand our still poor knowledge on the cell structure and biology of Sanchytriomycota.

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Data Availability

The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

aw :

algal wall

ax :

axosome

c :

centriole (nonfunctional kinetosome)

ch :

chloroplast

cv :

contractile vacuole

cw :

cyst wall

f :

filopodia

ga :

Golgi apparatus

hn :

host nucleus

k :

kinetosome

l :

lipid globule

la :

lamellipodium

m :

mitochondrium

mf :

microfilaments

mi :

microbody

mt :

microtubules

n :

nucleus

p :

papilla

pl :

plasma membrane

pc :

pseudocilium

sp :

sporangium

sw :

sporangium wall

tf :

transitional fibrill

tp :

transversal plate

tv :

transporting vesicle in pseudocilium

va :

vacuole

zo :

zoospores

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Acknowledgements

We are grateful to Dr. V.V. Aleshin for molecular data interpretation and discussion. We thank the Center for Molecular and Cell Technologies of Research Park SPbSU for providing sequencing and electron microscopic resources for this project. This work was made in the frame of lab topic 1021051402849-1 ZIN RAS and supported by RSF grant 21-74-20089 (Ribosomal gene sequencing and analysis, TEM observations and analysis, and manuscript writing and discussion). Sanchytrid cultivation was supported by grant of the Ministry of Science and Higher Education of the Russian Federation 075-15-2021-1069.

Funding

This work was supported by the RSF grant 21-74-20089 and by the grant of the Ministry of Science and Higher Education of the Russian Federation 075-15-2021-1069.

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Authors and Affiliations

  1. St. Petersburg State University, St. Petersburg, Russian Federation, 199034

    Andrey E. Vishnyakov, Alexei O. Seliuk & Victoria S. Tcvetkova

  2. Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russian Federation, 199034

    Sergey A. Karpov

Authors
  1. Andrey E. Vishnyakov
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  2. Alexei O. Seliuk
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  3. Victoria S. Tcvetkova
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  4. Sergey A. Karpov
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Contributions

Electron microscopic observations and analyses were performed by Andrey Vishnyakov and Sergey Karpov. Light microscopic observations and sequencing were performed by Aleksei Seliuk. Sample collection, isolation, and cultivation were performed by Victoria Tcvetkova. All authors contributed to the study conception and design. The first draft of the manuscript was written by Sergey Karpov and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Sergey A. Karpov.

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Vishnyakov, A.E., Seliuk, A.O., Tcvetkova, V.S. et al. Study of a new strain of Sanchytrium tribonematis expands our knowledge on Sanchytriomycota (Fungi). Mycol Progress 22, 69 (2023). https://doi.org/10.1007/s11557-023-01917-1

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  • DOI: https://doi.org/10.1007/s11557-023-01917-1

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