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Callus Growth Kinetics of Physic Nut (Jatropha curcas L.) and Content of Fatty Acids from Crude Oil Obtained In Vitro

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Abstract

The callus growth kinetics allows identifying the appropriate moment for callus pealing and monitoring the accumulation of primary and secondary metabolites. The physic nut (Jatropha curcas L.) is a plant species used for biofuel production due to its high oil content; however, this plant presents a great amount of bioactive compounds which can be useful for industry. The aim of this research was to establish a calli growth curve and to evaluate the fatty acid profile of crude oil extracted from callus. The callus growth kinetics presented a sigmoid standard curve with six distinct phases: lag, exponential, linear, deceleration, stationary, and decline. Total soluble sugars were higher at the inoculation day. Reducing sugars were higher at the inoculation day and at the 80th day. The highest percentage of ethereal extract (oil content) was obtained at the 120th day of culture, reaching 18 % of crude oil from the callus. The calli produced medium-chain and long-chain fatty acids (from 10 to 18 carbon atoms). The palmitic acid was the fatty acid with the highest proportion in oil (55.4 %). The lipid profile obtained in callus oil was different from the seed oil profile.

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Acknowledgments

The authors thank CNPq (the National Council for Scientific and Technological Development) and CAPES (Coordination for the Improvement of Higher Level -or- Education - Personnel) for the Post-Doctoral, Ph. D., and M. Sc. fellowships. Moreover, the authors thank the Fundação Araucária for supporting this research.

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Authors and Affiliations

  1. Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, Brazil

    Jefferson da Luz Costa, André Luís Lopes da Silva, Mário César Jucoski Bier, André Luiz Gollo, Luiz Alberto Junior Letti & Carlos Ricardo Soccol

  2. Department of Agricultural Sciences, Federal University of Tocantins, Gurupi, Brazil

    Eduardo Andrea Lemus Erasmo

  3. College of Forestry Engineering, Federal University of Mato Grosso, Cuiabá, Brazil

    Gilvano Ebling Brondani

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  1. Jefferson da Luz Costa
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Correspondence to Carlos Ricardo Soccol.

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da Luz Costa, J., da Silva, A.L.L., Bier, M.C.J. et al. Callus Growth Kinetics of Physic Nut (Jatropha curcas L.) and Content of Fatty Acids from Crude Oil Obtained In Vitro. Appl Biochem Biotechnol 176, 892–902 (2015). https://doi.org/10.1007/s12010-015-1618-y

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