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Transcriptome dynamics underlying elicitor-induced defense responses against Septoria leaf spot disease of tomato (Solanum lycopersicum L.)

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Abstract

Elicitor-induced defense response against potential plant pathogens has been widely reported in several crop plants; however, transcriptome dynamics underlying such defense response remains elusive. Our previous study identified and characterized a novel elicitor, κ-carrageenan, from Kappaphycus alvarezii, a marine red seaweed. Our preliminary studies have shown that the elicitor-treatment enhances the tolerance of a susceptible tomato cultivar to Septoria lycopersici (causative agent of leaf spot disease). To gain further insights into the genes regulated during elicitor treatment followed by pathogen infection, we have performed RNA-Seq experiments under different treatments, namely, control (untreated and uninfected), elicitor treatment, pathogen infection alone, and elicitor treatment followed by pathogen infection. To validate the results, forty-three genes belonging to five different classes, namely, ROS activating and detoxifying enzyme encoding genes, DEAD-box RNA helicase genes, autophagy-related genes, cysteine proteases, and pathogenesis-related genes, were chosen. Expression profiling of each gene was performed using qRT-PCR, and the data was correlated with the RNA-seq data. Altogether, the study has pinpointed a repertoire of genes that could be potential candidates for further functional characterization to provide insights into novel elicitor-induced fungal defense and develop transgenic lines resistant to foliar diseases.

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Data availability

The sequencing data associated with this work have been submitted to the NCBI SRA (Bioproject number is PRJNA622013).

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Acknowledgements

The authors thank The Director, CAS in Botany, University of Madras, for providing the laboratory facility. The corresponding author thank Department of Science and Technology, Science and Engineering Research Board (DST-SERB) for financial support.

Funding

The funding was provided by Department of Science and Technology, Science and Engineering Research Board, Govt. of India (File No.: ECR/2016/000165).

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Authors and Affiliations

  1. Unit of Plant Pathology, Center for Advanced Studies in Botany, University of Madras, Tamil Nadu, Guindy Campus, Chennai, 600 025, India

    Sumithra Devi Mani, Muthukumar Govindan & Radhakrishnan Nagarathnam

  2. National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110 067, India

    Saurabh Pandey

  3. Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, Telangana, India

    Mehanathan Muthamilarasan

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  1. Sumithra Devi Mani
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  2. Saurabh Pandey
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  3. Muthukumar Govindan
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  4. Mehanathan Muthamilarasan
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  5. Radhakrishnan Nagarathnam
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Contributions

RN conceived and designed the experiments. SDM, SP, MG performed the experiments. MM, RN analyzed the data. MM, RN wrote the manuscript. All authors have read and approved the final version of this manuscript.

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Correspondence to Radhakrishnan Nagarathnam.

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Mani, S.D., Pandey, S., Govindan, M. et al. Transcriptome dynamics underlying elicitor-induced defense responses against Septoria leaf spot disease of tomato (Solanum lycopersicum L.). Physiol Mol Biol Plants 27, 873–888 (2021). https://doi.org/10.1007/s12298-021-00970-y

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