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Infection process of Pseudocercospora musae on banana leaf

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Abstract

Yellow Sigatoka that is caused by Pseudocercospora musae is an important banana disease. The aim of this study was to elucidate the infection process of P. musae in banana leaves by scanning electron microscopy. Leaf samples were inoculated on the abaxial surface with P. musae and then analysed at 12, 24, 36, 48, 72, 96, 120, 144, and 168 h post inoculation (hpi) and at 36 and 50 days post inoculation (dpi). The conidia were found to be germinated between 24 and 36 hpi and penetrated through the stomata between 96 and 120 hpi, or more generally from 144 hpi. P. musae colonized the spongy parenchyma at 36 dpi and the palisade parenchyma at 50 dpi. Sporulation occurred at 50 dpi on the adaxial surface of leaves through the emergence of conidia on conidiophores through the stomata. Considering the importance of yellow Sigatoka in banana production, our results provide a better understanding of the life cycle of the fungus for treatment processes.

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References

  • Aman, M., & Rai, V. R. (2015). Potent toxigenic effect of Mycosphaerella musicola on locally growing banana varieties. Phytoparasitica, 43(3), 295–301.

    Article  CAS  Google Scholar 

  • Araujo, A. G., Val, A. D. B., Soares, J. D. R., et al. (2014). Host–pathogen interactions of Musa spp. and Mycosphaerella musicola with epidemiological variables and leaf anatomy within the pathosystem of yellow Sigatoka disease. Australian Journal of Crop Science, 8(8), 1200–1209.

    Google Scholar 

  • Babu, A. M., Kumar, V., & Govindaiah. (2002). Surface ultrastructural studies on the infection process of Pseudocercospora mori causing grey leaf spot disease in mulberry. Mycological Research, 106(08), 938–945.

    Article  Google Scholar 

  • Babu, A. M., Philip, T., Kariappa, B. K., & Kamble, C. K. (2009). Scanning electron microscopy of the infection process of Cercospora henningsii on cassava leaves. Journal of Phytopathology, 157(1), 57–62.

    Article  Google Scholar 

  • Babu, A. M., Philip, T., & Kumar, V. (2007). Development of the leaf spot fungus, Cercospora ricinella, on castor leaf – An SEM account. Journal of Phytopathology, 155(7–8), 426–430.

    Article  Google Scholar 

  • Beckman, P. M., & Payne, G. A. (1982). External growth, penetration, and development of Cercospora zeae-maydis in corn leaves. Phytopathology, 72(7), 810–815.

    Article  Google Scholar 

  • Borges Neto, C. R., Silveira, E. B., Mello, S. C. M., & Fontes, E. M. G. (1998). Scanning electron microscopy of the infection process of Cercospora caricis on purple nutsedge. Fitopatologia Brasileira, 23, 169–172.

    Google Scholar 

  • Burt, J. A., Rutter, J., & Gonzalez, H. (1997). Short distance wind dispersal of the fungal pathogens causing Sigatoka diseases in banana and plantain. Plant Pathology, 46(4), 451–458.

    Article  Google Scholar 

  • Castelan, F. P., Abadie, C., Hubert, O., Chilin-Charles, Y., De Bellaire, L. L., & Chillet, M. (2013). Relation between the severity of Sigatoka disease and banana quality characterized by pomological traits and fruit green life. Crop Protection, 50, 61–65.

    Article  Google Scholar 

  • Chang, T. C., Salvucci, A., Crous, P. W., & Stergiopoulos, I. (2016). Comparative genomics of the Sigatoka disease complex on banana suggests a link between parallel evolutionary changes in Pseudocercospora fijiensis and Pseudocercospora eumusae and increased virulence on the banana host. PLoS Genetics, 12(8), 1–32.

    Article  Google Scholar 

  • Cordeiro, Z. J. M., & Matos, A. P. (2005). Expressão da resistência de variedades de banana à Sigatoka-amarela. Fitopatologia Brasileira, 30(5), 532–534.

    Article  Google Scholar 

  • Cordeiro, Z. J. M., Rocha, H. S., & Araújo, A. G. (2011). Metodologia para manuseio de Mycosphaerella musicola em laboratório. EMBRAPA Mandioca e Fruticultura, Cruz das Almas.

  • Costa, F. H. S., Castro, E. M., Pasqual, M., Pereira, J. E. S., & Oliveira, C. (2009). Alterações anatômicas de bananeiras micropropagadas em resposta a aclimatização ex vitro. Ciência Rural, 39(2), 386–392.

    Article  Google Scholar 

  • Curvelo, C. R. S., Rodrigues, F. A., Berger, P. G., & Rezende, D. C. (2010). Microscopia eletrônica de varredura do processo infeccioso de Ramularia areola em folhas de algodoeiro. Tropical Plant Pathology, 35(2), 108–113.

    Article  Google Scholar 

  • Freitas, A. S., Pozza, E. A., Alves, M. C., Coelho, G., Rocha, H. S., & Pozza, A. A. A. (2015a). Spatial distribution of yellow Sigatoka leaf spot correlated with soil fertility and plant nutrition. Precision Agriculture, 17(1), 93–107.

    Article  Google Scholar 

  • Freitas, A. S., Pozza, E. A., Pozza, A. A. A., Oliveira, M. G. F., Silva, H. R., Rocha, H. S., & Galvão, L. R. (2015b). Impact of nutritional deficiency on yellow Sigatoka of banana. Australasian Plant Pathology, 44(4), 583–590.

    Article  CAS  Google Scholar 

  • Gasparotto, L., Pereira, J. C. R., Hanada, R. E., & Montarroyos, A. V. V. (2006). Sigatoka-negra da bananeira. Manaus: Embrapa Amazônia Ocidental.

    Google Scholar 

  • Gomes, L. I. S., Douhan, G. W., Bibiano, L. B., Maffia, L. A., & Mizubuti, E. S. (2013). Mycosphaerella musicola identified as the only pathogen of the Sigatoka disease complex present in Minas Gerais state, Brazil. Plant Disease, 97(12), 1537–1543.

    Article  Google Scholar 

  • Goos, R. D., & Tschirch, M. (1963). Greenhouse studies on the Cercospora leaf spot of banana. Transactions of the British Mycological Society, 46(3), 321–330.

    Article  Google Scholar 

  • Gupta, V. P., Tewari, S. K., Govindaiah, B. A. K., & Datta, R. K. (1995). Observations on the surface ultrastructure of conidial stage of Cercospora moricola and its infection process in mulberry. Sericologia, 35(1), 123–128.

    Google Scholar 

  • Hanada, R. E., Gasparotto, L., & Pereira, J. C. R. (2002). Esporulação de Mycosphaerella fijiensis em diferentes meios de cultura. Fitopatologia Brasileira, 27(2), 170–173.

    Article  Google Scholar 

  • Jacome, L. H., Schuh, W., & Stevenson, R. E. (1991). Effect of temperature and relative humidity on germination and germ tube development of Mycosphaerella fijiensis Var. difformis. Phytopathology, 81(12), 1480–1485.

    Article  Google Scholar 

  • Marschner, H. (2012). Mineral nutrition of higher plants. San Diego: Academic.

    Google Scholar 

  • Meredith, D. S., & Lawrence, J. S. (1969). Black leaf streak disease of bananas (Mycosphaerella fijiensis): Symptoms of disease in Hawaii, and notes on the conidial state of the causal fungus. Transactions of the British Mycological Society, 52(3), 459–476.

    Article  Google Scholar 

  • Miles, A. K., Akinsanmi, O. A., Sutherland, P. W., Aitken, E. A. B., & Drenth, A. (2009). Infection, colonisation and sporulation by Pseudocercospora macadamiae on macadamia fruit. Australasian Plant Pathology, 38(1), 36–43.

    Article  Google Scholar 

  • Noorulla, H., Naik, M. K., Gururaj, S., & Amaresh, Y. S. (2013). Prevalence of Sigatoka disease of banana in major growing areas of Karnataka, India. Asian Journal of Science and Technology, 4(08), 042–045.

    Google Scholar 

  • Passos, M. A., Cruz, V. O., Emediato, F. L., et al. (2013). Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycosphaerella musicola: Gene assembly, annotation and marker development. BMC Genomics, 14(78), 1–17.

    Google Scholar 

  • Paula, P. V. A. A., Pozza, E. A., Santos, L. A., Teixeira, O. H. S., & Paula, J. C. A. (2015). Formas de penetração do gênero Cercospora. Nucleus, 12(2), 271–280.

    Article  Google Scholar 

  • Rocha, H. S., Pozza, E. A., Uchôa, C. D. N., Cordeiro, Z. J. M., Souza, P. E., Sussel, Â. A. B., & Rezende, C. A. (2012). Temporal progress of yellow Sigatoka and aerobiology of Mycosphaerella musicola spores. Journal of Phytopathology, 160(6), 277–285.

    Article  Google Scholar 

  • Sandoval, J. A., Müller, L. E., & Weberling, F. (1994). Foliar morphology and anatomy of Musa cv. Grande Naine (AAA) plants grown in vitro and during hardening as compared to field-grown plants. Fruits, 49(1), 37–46.

    Google Scholar 

  • Singh, S. K., & Bhalla, K. (2000). New Pseudocercospora species causing foliar diseases in plain forests of Vindhya region in India. Indian Phytopathology, 53(4), 399–403.

    Google Scholar 

  • Souza, A. G. C., Rodrigues, F. Á., Maffia, L. A., & Mizubuti, E. S. G. (2011). Infection process of Cercospora coffeicola on coffee leaf. Journal of Phytopathology, 159(1), 6–11.

    Article  Google Scholar 

  • Stover, R. H. (1970). Leaf spot of bananas caused by Mycosphaerella musicola: Role of conidia in epidemiology. Phytopathology, 60(5), 856–860.

    Article  Google Scholar 

  • Surridge, A. K. J., Viljoen, A., Crous, R. W., & Wehner, F. C. (2003). Identification of the pathogen associated with Sigatoka disease of banana in South Africa. Australasian Plant Pathology, 32(1), 27–31.

    Article  Google Scholar 

  • Wharton, P. S., Julian, A. M., & O'Connell, R. J. (2001). Ultrastructure of the infection of Sorghum bicolor by Colletotrichum sublineolum. Phytopathology, 91(2), 149–158.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors acknowledge the National Council for Scientific and Technological Development (CNPq) for a PhD scholarship to the first author and a productivity grant to the second and last authors; the Higher Education Personnel Coordination (CAPES); the Foundation for Research Support of the State of Minas Gerais (FAPEMIG); the Federal University of Lavras (UFLA) for financing the research; and the Laboratory of Electron Microscopy and Ultrastructural Analysis of UFLA for their technical support.

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Authors and Affiliations

  1. Plant Pathology Department, Federal University of Lavras, PO Box 3037, CEP, Lavras, MG, 37200-000, Brazil

    Aurivan Soares Freitas, Edson Ampélio Pozza, Silvino Intra Moreira, Maria Gilmara de Oliveira Soares, Humberson Rocha Silva & Eduardo Alves

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  1. Aurivan Soares Freitas
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  2. Edson Ampélio Pozza
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  3. Silvino Intra Moreira
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  4. Maria Gilmara de Oliveira Soares
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  5. Humberson Rocha Silva
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  6. Eduardo Alves
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Correspondence to Aurivan Soares Freitas.

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Freitas, A.S., Pozza, E.A., Moreira, S.I. et al. Infection process of Pseudocercospora musae on banana leaf. Phytoparasitica 45, 317–324 (2017). https://doi.org/10.1007/s12600-017-0596-8

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  • DOI: https://doi.org/10.1007/s12600-017-0596-8

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