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Endophytism and bioactivity of endophytic fungi isolated from Combretum lanceolatum Pohl ex Eichler

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Abstract

We report the isolation and identification of endophytic fungi from Combretum lanceolatum Pohl ex Eichler. Further, we evaluated the relationships of fungi with the host plant and tested bioactivities of isolates. The fungi were isolated from disinfected root fragments and plated onto potato dextrose agar. Root pieces were also used to quantify fungal structures associated with the roots. Identification of fungi was carried out by characterization of morphological features and sequencing of the ITS region. Endophytism was confirmed by inoculation of endophyte-free seedlings followed by microscopic examination. The extract was obtained by maceration of the mycelium in ethyl acetate for antioxidant and antimicrobial evaluations. A total of 112 strains belonging to nine different species were isolated, the major classes were Dothideomycetes and Sordariomycetes. C. lanceolatum is colonized by dark septate endophytes (DSE), evidenced by the presence of microsclerotia and melanized hyphae. There is also co-colonization with mycorrhizal fungi in the same root fragments. Seedling inoculation experiments revealed that C. perangustum-95C and M. phaseolina-46C showed association with the seedlings of C. lanceolatum and differentiated microsclerotia and dark septate hyphae, indicating that these species are DSE. In the antimicrobial test, the D. phaseolorum-92C extract had the highest zones of inhibition against S. aureus and E. coli, respectively. The results showed that 100 % of the extracts have antioxidant activity ranging from low to moderate. All endophyte species had antioxidant and antimicrobial activities that were directly proportional to the dose-responses. Future research will involve chemical characterization and structural elucidation of bioactive compounds.

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Acknowledgments

This study was conducted using resources from different funding agencies FAPEMAT, CNPq, CAPES and the National Institute of Science and Technology in Wetlands (INAU).

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Authors and Affiliations

  1. Program of Graduate Studies in Biology and Biotechnology of Microorganisms, University State of Santa Cruz, Ilhéus, BA, Brazil

    Kátia Aparecida de Siqueira

  2. Graduate Studies in Chemistry Program, Federal University of Mato Grosso, Cuiabá, MT, Brazil

    Elson Rudimar Brissow

  3. Department of Biological Sciences, University State of Santa Cruz, Ilhéus, BA, Brazil

    Jane Lima dos Santos

  4. Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ, USA

    James Francis White

  5. Department of Basic Sciences in Health University, Federal University of Mato Grosso, Cuiabá, MT, Brazil

    Fabrício Rios Santos

  6. Department of Botany and Ecology, Federal University of Mato Grosso, Cuiabá, MT, Brazil

    Euziclei Gonzaga de Almeida & Marcos Antônio Soares

Authors
  1. Kátia Aparecida de Siqueira
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  2. Elson Rudimar Brissow
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  3. Jane Lima dos Santos
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  4. James Francis White
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  5. Fabrício Rios Santos
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  6. Euziclei Gonzaga de Almeida
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  7. Marcos Antônio Soares
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Corresponding author

Correspondence to Marcos Antônio Soares.

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de Siqueira, K.A., Brissow, E.R., Santos, J.L.d. et al. Endophytism and bioactivity of endophytic fungi isolated from Combretum lanceolatum Pohl ex Eichler. Symbiosis 71, 211–222 (2017). https://doi.org/10.1007/s13199-016-0427-6

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  • DOI: https://doi.org/10.1007/s13199-016-0427-6

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