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Multidisciplinary approach to describe Trebouxia diversity within lichenized fungi Buellia zoharyi from the Canary Islands

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Abstract

The Canary Islands are famous for their extraordinary biodiversity; however, lichenized algae have only been studied partially. Buellia zoharyi is a circum-Mediterranean/Macaronesian species that usually occurs in semi-arid areas of the Mediterranean, but occasionally some interesting communities of this species grow on basaltic lava flows in Lanzarote, Fuerteventura and Tenerife. Those three locations showed similar ecological conditions, but different mean annual temperatures. Here we applied a multidisciplinary approach to describe microalgae diversity from B. zoharyi covering the entire described range of distribution in the Canary Islands. Photobionts were characterized in symbiosis using molecular and microscopic techniques. Different Trebouxia spp. were detected as primary photobiont in each island (Trebouxia cretacea-Fuerteventura, T. asymmetrica-Lanzarote and Trebouxia sp. `arnoldoi´-Tenerife). Coexistence of various Trebouxia spp. within a thallus were detected by using specific primers-PCR. Those three photobionts were isolated and cultured under laboratory conditions. Different phytohormone profiles were obtained in the isolated strains which suggest different internal signalling needs. In addition, we characterized the response of the isolated strains to different temperatures using chlorophyll fluorescence. T. asymmetrica did not modify their Fv/fm values with respect to temperature acclimation. In contrast, Trebouxia sp. `arnoldoi’and T. cretacea were more sensitive to changes in growing temperature decreasing Fv/fm at 17 °C. Our results indicate that B. zoharyi is flexible regarding the photobiont choice depending on the region, and suggest that bioclimatic factors could influence the myco/photobiont association patterns.

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Acknowledgments

Dr. Arnoldo Santos was involved in the design of the surveys and the sample collection, also in the ecology and bioclimatic information of the locations. Daniel Sheerin revised the English manuscript. We dedicate this article to Eva Barreno in honour of her retirement.

Funding

Funding for field and laboratory work for this study was provided by the Ministerio de Economia y Competitividad (MINECO and FEDER, Spain) (CGL2016–79158-P) and Prometeo Excellence in Research Program (Generalitat Valenciana, Spain) (PROMETEOII/2013/021; PROMETEO/2017/039). Daniel Sheerin revised the English manuscript.

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Authors and Affiliations

  1. Botánica, ICBIBE, Fac. CC. Biológicas, Universitat de València, C/ Dr. Moliner, 50, 46100, Burjassot, Valencia, Spain

    Arantzazu Molins, Salvador Chiva, José Reig-Armiñana & Patricia Moya

  2. Centro de Citricultura y Producción Vegetal, Departamento de Horticultura, Instituto Valenciano de Investigaciones Agrarias, CV-315, Km 10,7, 46113, Moncada, Valencia, Spain

    Ángeles Calatayud

  3. Universitat de València, Estructura de Recerca Interdisciplinar en Biotecnologia i Biomedicina (ERI BIOTECMED), C/ Dr. Moliner 50, 46100, Burjassot, Spain

    Francisco Marco & Pedro Carrasco

  4. Laboratorio de Anatomía “Julio Iranzo”, Jardín Botánico de la Universitat de València, C/ Quart, 81, 46008, Valencia, Spain

    Francisco García-Breijo & José Reig-Armiñana

  5. Dpto. de Ecosistemas Agroforestales, ETSIAMN, Universitat Politècnica de València, Camino de Vera s/n, 46022, Valencia, Spain

    Francisco García-Breijo

Authors
  1. Arantzazu Molins
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  2. Salvador Chiva
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  3. Ángeles Calatayud
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  4. Francisco Marco
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  5. Francisco García-Breijo
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  6. José Reig-Armiñana
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  7. Pedro Carrasco
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  8. Patricia Moya
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Contributions

PM, AM and SC conceived the study and designed the laboratory part of the study. SC, PM and AM carried out laboratory work. FG-B and JR-A analyzed microscopic images. AC and PM performed photosynthesis measurements. FM and PC performed phytohormone quantifications. AM and PM analyzed all data and wrote the manuscript. All authors edited and approved the final version of the manuscript.

Corresponding author

Correspondence to Patricia Moya.

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Competing interests

The authors declare no competing interests.

Code availability

LSU rDNA: MT458607-MT458609; nrITS MT458610-MT458618

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Molins, A., Chiva, S., Calatayud, Á. et al. Multidisciplinary approach to describe Trebouxia diversity within lichenized fungi Buellia zoharyi from the Canary Islands. Symbiosis 82, 19–34 (2020). https://doi.org/10.1007/s13199-020-00722-8

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