Abstract
The genus Phylloporus has a worldwide distribution and although members of it are known to be ectomycorrhizal associates of several tree families, their ectomycorrhizas and specific symbiotic interactions are almost unknown. In this study, basidiomes and ectomycorrhizas of Phylloporus were sampled in two monodominant tropical areas from Quercus sapotifolia and Quercus oleoides in eastern Mexico. Mycorrhizal root tips were characterized morpho-anatomically and using molecular method. Phylloporus rimosus and Phylloporus sp. were identified as ectomycorrhizas after bioinformatic analysis of the internal transcribed spacer (ITS) region and large subunit (28S) of nuclear ribosomal DNA and translation elongation factor 1α (tef1-α) region of root-tips. The morphoanatomical description of these two ectomycorrhizas are presented here. Although there was presence of Phylloporus basidiomes in the two areas where Q. sapotifolia and Q. oleoides trees were dominant, the presence of belowground ectomycorrhizas was scarce. They were only found in a pure stand of Q. sapotifolia. This is the first work that confirms from both morphoanatomical and molecular evidence the ectomycorrhizal association of members of the genus Phylloporus. Moreover, we proved the interaction of Quercus sapotifolia from the tropical Forest of eastern Mexico with two species of Phylloporus.
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Data Availability
The sequences generated in this study are available in the NCBI GenBank (https://www.ncbi.nlm.nih.gov/genbank/) under the accession numbers explained in the text.
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Acknowledgements
This research was supported by CONAHCYT (FORDECYT-PRONACES/CF 263959).
Assistance in the field and laboratory was provided by D. Ramos, La González, and P. Susan assisted us with some molecular procedures, both at Instituto de Ecología, A.C.
Funding
This study was funded by CONAHCYT (FORDECYT-PRONACES/CF 263959).
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Antero Ramos, Leticia Montoya and Victor Bandala contributed to the study conceptualization, methodology, analyses and investigation. Antero Ramos wrote a first draft of the article and Leticia Montoya and Victor Bandala added information. All authors read and approved the final manuscript. All authors applied for funding acquisition.
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Ramos, A., Leticia, M. & Bandala, V.M. Morphological and Molecular Characterization of Ectomycorrhizas of Phylloporus (Boletales) and Quercus sapotifolia from Tropical Oak Forest of Eastern Mexico. Symbiosis 91, 45–54 (2023). https://doi.org/10.1007/s13199-023-00943-7
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DOI: https://doi.org/10.1007/s13199-023-00943-7