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Integrated mRNA and microRNA transcriptome analyses provide insights into paclobutrazol inhibition of lateral branching in herbaceous peony

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Abstract

Herbaceous peony (Paeonia lactiflora Pall.) is a new high-end cut flower, but a large number of lateral branches often appear in some excellent cultivars, which is inconvenient for cut flower production. In the present study, we analyzed the effects of paclobutrazol (PBZ) on the lateral branches of P. lactiflora and adopted a next-generation sequencing approach to identify miRNAs and mRNAs that were differentially expressed involved in the PBZ response. Our results indicate that PBZ may inhibit the production of lateral branches on P. lactiflora. There were 827 differentially expressed genes (DEGs) and 104 differentially expressed miRNAs (DEMs). Integrative analysis revealed 29 miRNA–mRNA interactions related to PBZ stress. Our results provided a wealth of genetic information and data on metabolic pathways for revealing the regulatory mechanism of PBZ inhibition of the development of lateral branches in P. lactiflora and provided a new possibility for reducing lateral branch formation in the production of herbaceous peony cut flowers.

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Acknowledgements

This study was supported by the Agricultural Science & Technology Independent Innovation Fund of Jiangsu Province (CX[14]2023), the Scientific Research Foundation for high level talents of Yangzhou University (137010420) and the Priority Academic Program Development from the Jiangsu Government.

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Authors and Affiliations

  1. College of Horticulture, Xinyang College of Agriculture and Forestry, Xinyang, 464000, China

    Lei Liu

  2. College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China

    Lei Liu & Jun Tao

  3. Jiangsu Key Laboratory of Crop Genetics and Physiology/College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China

    Lei Liu, Daqiu Zhao & Jun Tao

  4. Institutes of Agricultural Science and Technology Development, Yangzhou University/Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou, 225009, China

    Yanqing Wu

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  1. Lei Liu
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Contributions

DZ and JT conceived the study and designed the experiments; LL performed the experiments; LL and YW were responsible for data analysis and manuscript writing, with suggestions offered by JT. All authors read and approved the final manuscript.

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Correspondence to Jun Tao.

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The authors declare there are no competing interests.

Electronic supplementary material

Below is the link to the electronic supplementary material.

13205_2020_2489_MOESM1_ESM.jpg

Gene ontology functional classification of unigenes. The Y-axis indicates the number of a specific category of genes existed in main category (JPG 1218 kb)

13205_2020_2489_MOESM2_ESM.jpg

(a) EuKaryotic Ortholog Groups (KOG) and (b) Kyoto Encyclopedia of Genes and Genomes (KEGG) functional classifications of unigenes (JPG 1400 kb)

Venn diagram of differentially expressed genes (DEGs) in different comparisons (PDF 34 kb)

Gene-specific primers sequence for detection by qRT-PCR (DOC 23 kb)

The RNA classification statistics table (DOCX 13 kb)

Target genes of miRNAs identified in this study (XLSX 40 kb)

List of oppositely regulated miRNA–mRNA pairs in small RNA and transcriptome sequencing (XLSX 10 kb)

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Liu, L., Wu, Y., Zhao, D. et al. Integrated mRNA and microRNA transcriptome analyses provide insights into paclobutrazol inhibition of lateral branching in herbaceous peony. 3 Biotech 10, 496 (2020). https://doi.org/10.1007/s13205-020-02489-7

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