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Phyllosticta capitalensis, a widespread endophyte of plants

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Abstract

Phyllosticta capitalensis is an endophyte and weak plant pathogen with a worldwide distribution presently known from 70 plant families. This study isolated P. capitalensis from different host plants in northern Thailand, and determined their different life modes. Thirty strains of P. capitalensis were isolated as endophytes from 20 hosts. An additional 30 strains of P. capitalensis from other hosts and geographic locations were also obtained from established culture collections. Phylogenetic analysis using ITS, ACT and TEF gene data confirmed the identity of all isolates. Pathogenicity tests with five strains of P. capitalensis originating from different hosts were completed on their respective host plants. In all cases there was no infection of healthy leaves, indicating that this endophyte does not cause disease on healthy, unstressed host plants. That P. capitalensis is often isolated as an endophyte has important implications in fungal biology and plant health. Due to its endophytic nature, P. capitalensis is commonly found associated with lesions of plants, and often incorrectly identified as a species of quarantine importance, which again has implications for trade in agricultural and forestry production.

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Acknowledgments

We are grateful to Dhanushka Udayanga and Sajeewa S.N. Maharachchikumbura for their assistance. This study was financially supported by the Thailand Research Fund through the Royal Golden Jubilee Ph. D. Program (Grant No. PHD/0198/2552) to S. Wikee and Kevin D. Hyde. The National Research Council of Thailand is thanked for the award of grant No 54201020004 to study the genus Phyllosticta in Thailand.

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Authors and Affiliations

  1. School of Science, Mae Fah Luang University, Chiangrai, 57100, Thailand

    Saowanee Wikee, Ekachai Chukeatirote & Kevin D. Hyde

  2. Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiangrai, 57100, Thailand

    Saowanee Wikee, Ekachai Chukeatirote & Kevin D. Hyde

  3. CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands

    Lorenzo Lombard & Pedro W. Crous

  4. Microbiology, Department of Biology, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands

    Pedro W. Crous

  5. Laboratory of Phytopathology, Wageningen University and Research Centre (WUR), Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Pedro W. Crous

  6. Graduate School of Bioresources, Mie University, Kurima-machiya 1577, Tsu, Mie, 514-8507, Japan

    Chiharu Nakashima

  7. Electron Microscope Center, Tokyo University of Agriculture, Sakuraoka 1-1-1, Setagaya, Tokyo, 156-8502, Japan

    Keiichi Motohashi

  8. Institute Ocean and Earth Sciences, Institute for Postgraduate Studies, University Malaya, Kuala Lumpur, 50603, Malaysia

    Siti A. Alias

  9. Landcare Research, Private Bag 92170, Auckland Mail Centre, Auckland, 1142, New Zealand

    Eric H. C. McKenzie

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  1. Saowanee Wikee
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Correspondence to Saowanee Wikee.

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Wikee, S., Lombard, L., Crous, P.W. et al. Phyllosticta capitalensis, a widespread endophyte of plants. Fungal Diversity 60, 91–105 (2013). https://doi.org/10.1007/s13225-013-0235-8

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