Abstract
The aim of this study is to determine the severity of the disease Septoria leaf spot in tomato plants, through computational analysis of digital images of leaves affected. We collected and obtained digital images of tomato leaves with absence and presence of the disease with varying degrees of severity. From a script written in R with the EBImage package, the image was decomposed into three levels of color (RGB) and, through the process of thresholding the image segmentation, was performed separating sheet and injuries in relation to the background, determining the percentage of damaged area. Statistical properties were extracted from the original images and, from them and the severity quantified by software, was realized the process of correlation and regression analysis to indicate a template that determines the percentage of damaged area through the properties of the images. Subsequently, these models were tested, with a new image bank, from the RMSE error measures. The methodology described, was able to identify and quantify the damaged areas of the leaves with symptoms of diseases, extract the statistical properties of the images as allowed to predict mathematical models with acceptable potential and quality for indirect determination of the percentage of injured area through the properties of the images.
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Appendices
Appendix 1 – Original images and images segmented by R software in relation to leaf and stain (necrosis + chlorosis) and their percentages of injured area
Appendix 2 – Script elaborated in R software for quantification of severity
Appendix 3 – Script elaborated in the R software for extracting the statistical properties of the image and correlation process
Appendix 4 – Key commands used and their functions
- BIC :
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Calculates the Bayesian information criterion.
- channel :
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Handles color space conversions between image modes.
- computeFeatures.shape :
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Calculates morphological and texture characteristics of image objects.
- colour :
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computes the correlation of x and y if these are vectors. If x and Y are arrays, then the correlation between the X columns and the y columns are computed
- display :
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Shows the created image.
- fillHull :
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Fills loopholes in objects.
- library :
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Loads packages into the software
- lm :
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Performs regression, analysis of variance and covariance.
- mean :
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Calculates the average of the indicated values.
- otsu :
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Returns a threshold value based on the Otsu method, wich can be used to reduce the grayscale image to a binary image.
- proc.time :
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Determines how long the system (in seconds) takes to run the process.
- readImage :
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Reads and writes images to/from and URLs.
- regsubsets :
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Multiple linear regression analysis for choosing a better model.
- Resize :
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Performs all transformations linears spatial: rotation, translation, resizing e transformation em general.
- rm :
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Removes specified objects.
- sd :
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Calculates the standard deviation.
- sum :
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Returns the sum of all vallues.
- write.xlsx :
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Saves data to a spread sheet xlsx.
- writeTIFF :
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Saves one or more bitmap images in the format TIFF.
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Mattos, A.d., Tolentino Júnior, J.B. & Itako, A.T. Determination of the severity of Septoria leaf spot in tomato by using digital images. Australasian Plant Pathol. 49, 329–356 (2020). https://doi.org/10.1007/s13313-020-00697-6
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DOI: https://doi.org/10.1007/s13313-020-00697-6