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Evaluation of chickpea (Cicer arietinum L.) irradiation-induced mutants for resistance to ascochyta blight in controlled environment

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Abstract

Induced mutation is one of the best alternatives for the improvement in chickpea resistance to ascochyta blight. This study was conducted to determine the lethal dose of 50% for the irradiation of chickpea seeds (LD50) and select chickpea mutant lines resistant to Ascochyta rabiei Pass. Irradiation of chickpea seeds with a range of gamma ray doses led to define the LD50 as 150 Gy. In addition, a genetic variability among plants derived by mutagenesis (M2 generation) was demonstrated by the ISSR technique. Among 36 studied mutant lines we selected three mutant lines resistant to A. rabiei in M2 and M3 generations.

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Acknowledgements

The stay of Dr. Yassine MABROUK at the University of Toulouse was financed by a Grant (85/TUN/31) from the Islamic Development Bank.

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Authors and Affiliations

  1. Laboratoire de Biotechnologies et Technologie Nucléaire, Centre National des Sciences et Technologies Nucléaires, 2020, Sidi Thabet, Tunisia

    Yassine Mabrouk & Kamel Charaabi

  2. Laboratoire de Biochimie et de Technobiologie, Faculté des Sciences de Tunis, Université de Tunis El-Manar, Campus Universitaire Farhat Hached, 2092, Tunis, Tunisia

    Yassine Mabrouk & Omrane Belhadj

  3. Laboratoire de Protection des Cultures, Faculté des Sciences de la Nature et de la Vie, Université Abdelhamid Ibn Badis de Mostaganem BP 188- 27000., BP 188- 27000, Mostaganem, Algeria

    Djamel Mahiout

  4. Ecolab, Université de Toulouse, CNRS, 18 Chemin de Borde Rouge, 31326, Castanet-Tolosan, France

    Martina Rickauer

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  1. Yassine Mabrouk
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  2. Kamel Charaabi
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  3. Djamel Mahiout
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  4. Martina Rickauer
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  5. Omrane Belhadj
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Correspondence to Yassine Mabrouk.

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Mabrouk, Y., Charaabi, K., Mahiout, D. et al. Evaluation of chickpea (Cicer arietinum L.) irradiation-induced mutants for resistance to ascochyta blight in controlled environment. Braz. J. Bot 41, 311–318 (2018). https://doi.org/10.1007/s40415-018-0458-8

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  • DOI: https://doi.org/10.1007/s40415-018-0458-8

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