Skip to main content
SpringerLink
Log in

Occurrence of Calonectria leaf blight in Eucalyptus benthamii progenies and potential for disease resistance

  • Original Article
  • Published:
Tropical Plant Pathology Aims and scope Submit manuscript

Abstract

Leaf blight caused by the fungi of the genus Calonectria represents a high economic risk for forestry companies due to the high potential of economic damage to commercial eucalyptus plantations. An outbreak of leaf blight was observed in Eucalyptus benthamii progeny in three sites in Southern Brazil. This study aimed to: (i) identify the causal agents of the disease; (ii) quantify the incidence and severity of leaf blight (CLB), and (iii) evaluate the selection for CLB resistance potential of E. benthamii. The incidence of the disease was 100% and severity progressed over time, causing more than 50% of canopy defoliation in most trees. The fungal species associated with this study were C. metrosideri and C. spathulata, both found to be pathogenic; C. spathulata was the most aggressive. Infection of E. benthamii by these fungi was associated with temperatures around 18 °C and relative humidity of approximately 74%. There is sufficient genetic variability for the selection of resistant E. benthamii progenies to CLB, based on low severity degrees.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Data availability

The datasets generated during and analyzed during the current study are available from the corresponding author on reasonable request.

References

  • Alfenas AC, Ferreira FA (2007) Inoculação de fungos fitopatogênicos. In: Alfenas AC, Mafia RG (eds) Métodos em Fitopatologia. Viçosa, Minas Gerais. UFV, pp 117–137

    Google Scholar 

  • Alfenas AC, Zauza EA, Mafia RG, Assis TF (2009) Clonagem e doenças do eucalipto, 2ª edn. Viçosa, Minas Gerais. UFV

  • Alfenas RF, Pereira OL, Ferreira MA, Jorge VL, Crous PW, Alfenas AC (2013) Calonectria metrosideri, a highly aggressive pathogen causing leaf blight, root rot, and wilt of Metrosideros spp. in Brazil. Forest Pathology 43:257–265

    Article  Google Scholar 

  • Alfenas RF, Lombard L, Pereira OL, Alfenas AC, Crous PW (2015) Diversity and potential impact of Calonectria species in Eucalyptus plantations in Brazil. Studies in Mycology 80:89–130

    Article  CAS  Google Scholar 

  • Alfenas RF, Freitas RG, Pereira OL, Coutinho MM, Zarpelon TG, Cândido TS, Alfenas AC (2016) Screening of Corymbia and Eucalyptus species for resistance to Calonectria pteridis leaf blight. Forest Pathology 46:76–81

    Article  Google Scholar 

  • Booth TH, Jovanovic T, Old KM, Dudzinski MJ (2000) Climatic mapping to identify high-risk areas for Cylindrocladium quinqueseptatum leaf blight on eucalypts in mainland South East Asia and around the world. Environmental Pollution 108:365–372

    Article  CAS  Google Scholar 

  • Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

    Article  CAS  Google Scholar 

  • Climate-data.org. (2020) Dados climáticos para cidades mundiais. Available at: https://pt.climate-data.org/. Accessed 17 May 2020

  • Crous PW (2002) Taxonomy and pathology of Cylindrocladium (Calonectria) and allied genera. Minnesota. APS Press, Saint Paul

    Google Scholar 

  • Crous PW, Wingfield MJ, Cheewangkoon R, Carnegie AJ, Burgess TI, Summerell BA, Edwards J, Taylor PWJ, Groenewald JZ (2019) Foliar pathogens of eucalypts. Studies in Mycology 94:125–298

    Article  CAS  Google Scholar 

  • Farris JS, Kallersjo M, Kluge AG, Bult C (1994) Testing significance of incongruence. Cladistics 10:315–319

    Article  Google Scholar 

  • Ferreira FA (1989) Patologia florestal - Principais doenças florestais no Brasil. Viçosa, Minas Gerais. Sociedade de Investigações Florestais

    Google Scholar 

  • Freitas RG, Alfenas RF, Guimarães LMS, Badel JL, Alfenas AC (2019) Genetic diversity and aggressiveness of Calonectria pteridis in Eucalyptus spp. Plant Pathology 68:869–877

    Article  Google Scholar 

  • Graça RN, Alfenas AC, Maffia LA, Titon M, Alfenas RF, Lau D, Rocabado JMA (2009) Factors influencing infection of eucalypts by Cylindrocladium pteridis. Plant Pathology 58:971–981

    Article  Google Scholar 

  • Gilmour AR, Gogel BJ, Cullis BR, Thompson R (2009) ASReml user guide release 3.0, VSN International Ltd, Hemel Hempstead

  • Hepperle D (2004) SeqAssem©. A sequence analysis tool, contig assembler and trace data visualization tool for molecular sequences. Available at: http://www.sequentix.de.>.

  • Hirooka Y, Takeuchi J, Horie H, Natsuaki KT (2008) Cylindrocladium brown leaf spot on Howea belmoreana caused by Calonectria ilicicola (anamorph: Cylindrocladium parasiticum) in Japan. Journal of General Plant Pathology 74:66–70

    Article  Google Scholar 

  • Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  CAS  Google Scholar 

  • IBÁ - Indústria brasileira de árvores (2019). Available at: https://iba.org/datafiles/publicacoes/relatorios/iba-relatorioanual2019.pdf>.

  • IEA. Preço eucalipto, ano 2018. Available at: https://www.noticiasagricolas.com.br/cotacoes/silvicultura/preco-eucalipto. Accessed 20 November 2020

  • Index Fungorum (2021). Available at: http://www.indexfungorum.org/Names/namesrecord.asp?RecordId=103106. Accessed 14 January 2021.

  • Larget B, Simon DL (1999) Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution 16:750–759

    Article  CAS  Google Scholar 

  • Li JQ, Wingfield MJ, Liu Q, Barnes I, Roux J, Lombard L, Crous PW, Chen S (2017) Calonectria species isolated from Eucalyptus plantations and nurseries in South China. IMA Fungus 8:259–286

    Article  Google Scholar 

  • Lombard L, Rodas CA, Crous PW, Wingfield BD, Wingfield MJ (2009) Calonectria (Cylindrocladium) species associated with dying Pinus cuttings. Persoonia 23:41–47

    Article  CAS  Google Scholar 

  • Lombard L, Wingfield MJ, Alfenas AC, Crous PW (2016) The forgotten Calonectria collection: Pouring old wine into new bags. Studies in Mycology 85:159–198

    Article  CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8:4321–4325

    Article  CAS  Google Scholar 

  • Nirenberg H (1981) A simplified method for identifying Fusarium spp. occurring on wheat. Canadian Journal of Botany 59:1599–1609

    Article  Google Scholar 

  • Office of Environment and Heritage (OEH). (2020) Camden white gum – profile. Available at: https://www.environment.nsw.gov.au/threatenedspeciesapp/profile.aspx?id=10284. Accessed 20 May 2020.

  • O’Donnell K, Cigelnik E (1997) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution 7:103–116

    Article  Google Scholar 

  • Pataky JK, Carson ML (2010) Resistência da planta hospedeira. In: Trigiano RN, Windham MT, Windham AS (eds) Fitopatologia: conceitos e exercícios de laboratório. RS. Artmed, Porto Alegre, pp 411–428

    Google Scholar 

  • Pham NQ, Barnes I, Chen S, Liu F, Dang QN, Pham TQ, Lombard L, Crous PW, Wingfield MJ (2019) Ten new species of Calonectria from Indonesia and Vietnam. Mycologia 111:1–25

    Article  Google Scholar 

  • Pires BM (2000) Efeito da desrama artificial no crescimento e na qualidade da madeira de Eucalyptus grandis para serraria. Tese de Doutorado, Universidade Federal de Viçosa. Viçosa, MG.

  • Polizzi G, Vitale A, Aiello D, Guarnaccia V, Crous P, Lombard L (2012) First report of Calonectria ilicicola causing a new disease on Laurus (Laurus nobilis) in Europe. Journal of Phytopathology 160:41–44

    Article  Google Scholar 

  • R Core Team (2018) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available online at https://www.R-project.org/.

  • Rehn VNC, Menezes M, Rehn KG, Coêlho RSB (2004) Isolation, morphological identification and pathogenicity of Cylindrocladium scoparium and C. clavatum isolates obtained from plants rhizosphere cultivated in Pernambuco State. Brazilian Journal of Microbiology 35:292–294

    Article  Google Scholar 

  • Rodas CA, Lombard L, Gryzenhout M, Slippers B, Wingfield MJ (2005) Cylindrocladium blight of Eucalyptus grandis in Colombia. Australasian Plant Pathology 34:143–149

    Article  Google Scholar 

  • Ronquist F, Teslenko M, PVD M, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61:539–542

    Article  Google Scholar 

  • Schultz B, Sbravatti JA Jr, Auer CG, Santos AF (2015) Impacto da mancha foliar causada por Cylindrocladium candelabrum em plantios jovens de Eucalyptus benthamii em Rio Negrinho - SC. Ciência Florestal 25:307–316

    Article  Google Scholar 

  • Silva DL, Higa AR, Santos AG (2012) Silvicultura e melhoramento genético do Eucalyptus benthamii. Curitiba, PR. FUPEF.

  • Soares ID, Auer CG, Santos AF, Tambarussi EV, Rezende EH, TAV C, Duin IM (2017) Fungos associados à mancha foliar em Eucalyptus benthamii Maiden et Cambage na região sul do Brasil. Biofix Scientific Journal 2:32–37

    Article  Google Scholar 

  • Soares ID, Auer CG, Santos AF, RAF S, TPF S, Ferreira MA (2019) First report of Calonectria metrosideri on Eucalyptus benthamii in Brazil. Plant Disease 103:2477

    Article  CAS  Google Scholar 

  • Wang Q, Liu Q, Chen S (2019) Novel species of Calonectria isolated from soil near Eucalyptus plantations in southern China. Mycologia 111:1028–1040

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank ‘Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil’ (CAPES) and ‘Conselho Nacional de Desenvolvimento Científico e Tecnológico’ (CNPq)-Brazil for research fellowship.

Author information

Author notes

  1. Izabele Domingues Soares Miranda

    Present address: Instituto de Estudos do Xingu, Universidade Federal do Sul e Sudeste do Pará, São Félix do Xingu, PA, 68380-000, Brazil

Authors and Affiliations

  1. Departamento de Ciências Florestais, Universidade Federal do Paraná, Curitiba, PR, 80210-170, Brazil

    Izabele Domingues Soares Miranda & Eduardo Henrique Rezende

  2. Laboratório de Patologia Florestal, Embrapa Florestas, Colombo, PR, 84311-000, Brazil

    Celso Garcia Auer & Álvaro Figueredo dos Santos

  3. Departamento de Fitopatologia, Universidade Federal de Lavras, Lavras, MG, 37200-900, Brazil

    Maria Alves Ferreira

  4. Departamento de Engenharia Florestal, Universidade Estadual do Centro-Oeste, Irati, PR, 84500-000, Brazil

    Evandro Vagner Tambarussi

  5. Departamento de Fitopatologia, Universidade de Brasília, Brasília, DF, 70910-900, Brazil

    Rildo Alexandre Fernandes da Silva

Authors
  1. Izabele Domingues Soares Miranda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Celso Garcia Auer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Álvaro Figueredo dos Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maria Alves Ferreira
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Evandro Vagner Tambarussi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rildo Alexandre Fernandes da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Eduardo Henrique Rezende
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Material preparation, data collection, and analysis were performed by Izabele D. S. Miranda, Celso G. Auer, Evandro V. Tambarussi, Eduardo H. Rezende, and Maria A. Ferreira. Rildo Alexandre F. da Silva and Álvaro F. dos Santos revised the work critically for important intellectual content. The first draft of the manuscript was written by the first author, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Izabele Domingues Soares Miranda.

Ethics declarations

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Miranda, I.D.S., Auer, C.G., dos Santos, Á.F. et al. Occurrence of Calonectria leaf blight in Eucalyptus benthamii progenies and potential for disease resistance. Trop. plant pathol. 46, 254–264 (2021). https://doi.org/10.1007/s40858-021-00426-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40858-021-00426-4

Keywords

Search

Navigation