Skip to main content
SpringerLink
Log in

First report of damping-off disease caused by Fusarium oxysporum in Pinus massoniana in China

  • Original Article
  • Published:
Journal of Plant Diseases and Protection Aims and scope Submit manuscript

Abstract

Masson pine (Pinus massoniana) is an important tree species of high economic value in southern China; however, between 2016 and 2018, the damping-off disease caused the death of whole Masson pine seedlings in Duyun, Guizhou Province, China. To improve the control and management of the disease, we identified symptoms and characterized the morphology, taxonomy and pathogenicity of the pathogens causing damping-off disease in P. massoniana in China. Infected seedlings exhibited needle yellowing and blight, extensive peeling and rot to the roots that led to the mortality of seedlings. We found that isolates causing damping-off disease in P. massoniana in China belonged to Fusarium oxysporum, based on morphology indicators and two genetic markers (internal transcribed spacer and β-tubulin gene regions). Symptoms of damping-off were reproduced in all seedlings inoculated with the isolates, and pathogenicity was found to be high. This study confirms for the first time that F. oxysporum is the causal agent of damping-off in Masson pine in China, and our findings will enable the development of more effective management strategies for this disease that causes damage to an economically important tree species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Agustí-Brisach C, Pérez-Sierra A, García-Figueres F, Montón C, Armengol J (2011) First report of damping-off caused by Cylindrocarpon pauciseptatum on Pinus radiata in Spain. Plant Dis 95:874

    Article  PubMed  Google Scholar 

  • Allen TW, Enebak SA, Carey WA (2004) Evaluation of fungicides for control of species of Fusarium on longleaf pine seed. Crop Prot 23:979–982

    Article  CAS  Google Scholar 

  • Altınok HH (2005) First report of fusarium wilt of eggplant caused by Fusarium oxysporum f. sp. melongenae in Turkey. Plant Pathol 54:577

    Article  Google Scholar 

  • Asaka O, Shoda M (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Appl Environ Microb 62:4081–4085

    Article  CAS  Google Scholar 

  • Babadoost M, Islam SZ (2003) Fungicide seed treatment effects on seedling damping-off of pumpkin caused by Phytophthora capsici. Plant Dis 87:63–68

    Article  CAS  PubMed  Google Scholar 

  • Berg LE, Miller SS, Dornbusch MR, Samac DA (2017) Seed rot and damping-off of alfalfa in Minnesota caused by Pythium and Fusarium species. Plant Dis 101:1860–1867

    Article  CAS  PubMed  Google Scholar 

  • Berruezo LA, Cardenas GEM, Harries ED, Stenglein SA, Curti RN, Rodriguero MS, Galvan MZ (2018) Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina. Eur J Plant Pathol 151:1065–1079

    Article  Google Scholar 

  • Bloomberg WJ (1971) Diseases of Douglas-fifir seedlings caused by Fusarium oxysporum. Phytopathology 61:467–470

    Article  Google Scholar 

  • Brownell KH, Schneider RW (1983) Fusarium hypocotyl rot of sugar pine in California forest nurseries. Plant Dis 67:105–107

    Article  Google Scholar 

  • Can C, Yucel S, Korolev N, Katan T (2004) First report of fusarium crown and root rot of tomato caused by Fusarium oxysporum f. sp. radicis-lycopersici in Turkey. Plant Pathol 53:814

    Article  Google Scholar 

  • Cerqueira A, Alves A, Berenguer H, Correia B, Gómez-Cadenas A, Diez JJ, Monteiro P, Pinto G (2017) Phosphite shifts physiological and hormonal profile of Monterey pine and delays Fusarium circinatum progression. Plant Physiol Bioch 114:88–99

    Article  CAS  Google Scholar 

  • Chakravarty P, Hwang SF (1991) Effect of an ectomycorrhizal fungus, Laccaria laccata, on Fusarium damping-off in Pinus banksiana seedlings. For Pathol 21:97–106

    Article  Google Scholar 

  • Claerbout J, Venneman S, Vandevelde I, Decombel A, Bleyaert P, Volckaert A, Neukermans J, Höfte M (2018) First report of Fusarium oxysporum f. sp. lactucae race 4 on lettuce in Belgium. Plant Dis 102:1037

    Article  Google Scholar 

  • Davydenko K, Nowakowska JA, Kaluski T, Gawlak M, Sadowska K, Garcia JM, Diez JJ, Okorski A, Oszako T (2018) A comparative study of the pathogenicity of Fusarium circinatum and other Fusarium species in polish provenances of P. sylvestris L. Forests 9:1–13

    Article  Google Scholar 

  • Du MF, Ding GJ, Cai Q (2018) The Transcriptomic responses of Pinus massoniana to drought stress. Forests 9:326

    Article  Google Scholar 

  • Enebak SA, Palmer MA, Blanchette RA (1990) Managing soil-borne pathogens of white pine in a forest nursery. Plant Dis 74:195–198

    Article  CAS  Google Scholar 

  • Fujinaga M, Ogiso H, Shinohara H, Tsushima S, Nishimura N, Togawa M, Saito H, Nozue M (2005) Phylogenetic relationships between the lettuce root rot pathogen Fusarium oxysporum f. sp. lactucae races 1, 2, and 3 based on the sequence of the intergenic spacer region of its ribosomal DNA. J Gen Plant Pathol 71(6):402–407

    Article  CAS  Google Scholar 

  • Gherbawy YA, El-tayeb MA, Maghraby TA, Shebany YM, El-deeb BA (2012) Response of antioxidant enzymes and some metabolic activities in wheat to Fusarium spp. Infect Acta Agron Hung 60:319–333

    Article  CAS  Google Scholar 

  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microb 61:1323–1330

    Article  CAS  Google Scholar 

  • Gordon TR, Swett CL, Wingfield MJ (2015) Management of Fusarium diseases affecting conifers. Crop Prot 73:28–39

    Article  Google Scholar 

  • Hanaa RMF, Abdou ZA, Salama DA, Ibrahim MAR, Sror HAM (2011) Effect of neem and willow aqueous extracts on fusarium wilt disease in tomato seedlings: Induction of antioxidant defensive enzymes. Ann Agric Sci 56:1–7

    Article  Google Scholar 

  • Hassan Dar Gh, Beig MA, Ahanger FA, Ganai NA, Ashraf Ahangar M (2011) Management of root rot caused by Rhizoctonia solani and Fusarium oxysporum in blue pine (Pinus wallichiana) through use of fungal antagonists. Asian J Plant Pathol 5:2

    Google Scholar 

  • Heather WA, Pratt BH, Chin TU (1977) Pre- and post-emergence damping off of seedlings of pinus species by phytophthora cinnamomi and Ph Drechsleri. Aust J Bot 25:385–393

    Article  Google Scholar 

  • Hernández-Lauzardo AN, Lopez P, Sanahuja G, Suarez S, Palmateer AJ (2018) First report of Fusarium oxysporum causing stem rot on Medinilla myriantha in Florida. Plant Dis 102:441

    Article  Google Scholar 

  • Herron DA, Wingfifield MJ, Wingfifield BD, Rodas CA, Marincowitz S, Steenkamp ET (2015) Novel taxa in the Fusarium fujikuroi species complex from Pinus spp. Stud Mycol 80:131–150

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • James RL (2012) Fusarium root and stem diseases. In: Cram MM, Frank MS, Mallams KM (eds) Forest nursery pests, agriculture handbook No. 680. USDA Forest Service, pp 117–120

  • James RL, Militante EP, Woo JY, Gilligan CJ (1986). Pathogenicity of fusarium from forest seedling nurseries on Douglas-fir and ponderosa pine seedlings. Forest Pest Management Report No. 86e8. USDA Forest Service, North Reg

  • Kang B, Liu SR, Zhang GG, Chang JG, Wen YG, Ma JM, Hao WF (2006) Carbon accumulation and distribution in Pinus massoniana and Cunninghamia lanceolata mixed forest ecosystem in Daqingshan, Guangxi, China. Acta Ecol Sin 26:1320–1327

    Article  CAS  Google Scholar 

  • Kim MS, Stewart JE, Dumroese RK, Klopfenstein NB (2012) Occurrence of the root rot pathogen, Fusarium commune, in forest nurseries of the midwestern and western United States. J Phytopathol 160:112–114

    Article  Google Scholar 

  • Lamichhane JR, Dürr C, Schwanck AA, Robin MH, Sarthou JP, Cellier V, Messéan A, Aubertot JN (2017) Integrated management of damping-off diseases. Rev Agron Sustain Dev 37:10

    Article  Google Scholar 

  • Laurence MH, Summerell BA, Burgess LW, Liew ECY (2014) Genealogical concordance phylogenetic species recognition in the Fusarium oxysporum species complex. Fungal Biol 118:374–384

    Article  PubMed  Google Scholar 

  • Lazreg F, Belabid L, Sanchez J, Gallego E, Garrido-Cardenas JA, Elhaitoum A (2013a) First report of Fusarium acuminatum causing damping-off disease on Aleppo pine in Algeria. Plant Dis 97:557

    Article  CAS  PubMed  Google Scholar 

  • Lazreg F, Belabid L, Sanchez J, Gallego E, Garrido-Cardenas JA, Elhaitoum A (2013b) First report of Fusarium redolens as a causal agent of Aleppo pine damping-Off in Algeria. Plant Dis 97:997

    Article  CAS  PubMed  Google Scholar 

  • Lazreg F, Belabid L, Sanchez J, Gallego E, Garrido-Cardenas JA, Elhaitoum A (2013c) First report of Fusarium chlamydosporum causing damping-off disease on Aleppo pine in Algeria. Plant Dis 97:1506

    Article  CAS  PubMed  Google Scholar 

  • Lazreg F, Belabid L, Sanchez J, Gallego E, Garrido-Cardenas JA, Elhaitoum A (2014) First report of Fusarium equiseti causing damping-off disease on Aleppo pine in Algeria. Plant Dis 98:1268

    Article  CAS  PubMed  Google Scholar 

  • Liang QX, Pan FY, Li DX (2002) Regularity of outbreak and control techniques of Pinus Massoniana Cercospora needle blight. J Zhejiang For Sci Technol 22:64–65

    Google Scholar 

  • Liu QH, Zhou ZC, Wei YC, Shen DY, Feng ZP, Hong SP (2015) Genome-wide identification of differentially expressed genes associated with the high yielding of oleoresin in secondary xylem of masson pine (Pinus massoniana Lamb) by transcriptomic analysis. PLoS ONE 10(7):e0132624

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Lombard L, Sandoval-Denis M, Lamprecht SC, Crou PW (2019) Epitypification of Fusarium oxysporum-clearing the taxonomic chaos. Persoonia 43:1–47

    Article  CAS  PubMed  Google Scholar 

  • Machón P, Pajares JA, Diez JJ, Alves-Santos FM (2009) Influence of the ectomycorrhizal fungus Laccaria laccataon pre-emergence, post-emergence and late damping-off by Fusarium oxysporum and F. verticillioides on Stone pine seedlings. Symbiosis 49:101–104

    Article  Google Scholar 

  • Maciel CG, Walker C, dos Santos RF, Muniz MFB, Brum DL (2017) Fusarium oxysporum and F. verticillioides associated with damping-off in Pinus spp. Rev Cienc Agron 48:134–141

    Article  Google Scholar 

  • Martín-Pinto P, Pajares J, Díez J (2008) Pathogenicity of Fusarium verticillioides and Fusarium oxysporum on Pinus nigra seedlings in northwest Spain. For Path 38:78–82

    Article  Google Scholar 

  • Morales-Rodriguez C, Bastianelli G, Aleandri M, Chilosi G, Vannini A (2018) Application of Trichoderma spp. complex and biofumigation to control damping-off of Pinus radiata D. Don caused by Fusarium circinatum Nirenberg and ODonnell. Forests 9(7):421

    Article  Google Scholar 

  • Robles Yerena L, Leyva Mir SG, Cruz Gómez A, Camacho Tapia M, Nieto Ángel D, Tovar Pedraza JM (2016) Fusarium oxysporum Schltdl. and Fusarium solani (Mart.) Sacc. Cause wilting of Pinus spp. seedlings in the nursery. Revista mexicana de ciencias forestales 7:25–36

    Article  Google Scholar 

  • Seseni L, Regnier T, Roux-Van Der Merwe MP, Mogale E, Badenhorst J (2014) Control of Fusarium spp. causing damping-off of pine seedlings by means of selected essential oils. Ind Crop Prod 76:329–332

    Article  CAS  Google Scholar 

  • Sun CJ (1992) Community forestry in southern China. J For 90:35–40

    Google Scholar 

  • Swett CL, Gordon TR (2013) Dualism in symbiosis: growth and defense enhancement of symptomless infection by the pathogen Fusarium circinatum in Pinus radiata seedlings. Phytopathology 103:191–191

    Google Scholar 

  • Tamura K, Stecher G, Peterson D, Lewis PO (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tint H (1945) Studies in the Fusarium damping-off of conifers. I. The comparative virulence of certain Fusaria. Phytopathology 35:421–439

    CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and amplifications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Wingfifield MJ, Hammerbacher A, Ganley RJ, Steenkamp ET, Gordon TR, Wingfifield BD, Coutinho TA (2008) Pitch canker caused by Fusarium circinatum: a growing threat to pine plantations and forests worldwide. Australas Plant Path 37:319–334

    Article  Google Scholar 

  • Xue Q, Xiang Y, Wu XQ, Li MJ (2019) Bacterial communities and virulence associated with pine wood nematode Bursaphelenchus xylophilus from different Pinus spp. Int J Mol Sci 20(13):3342

    Article  CAS  PubMed Central  Google Scholar 

  • Yu LM, Zhao MM, Wang JS, Cui C, Yang B, Jiang YM, Zhao QZ (2008) Antioxidant, immunomodulatory and anti-breast cancer activities of phenolic extract from pine (Pinus massoniana Lamb) bark. Innov Food Sci Emerg 9:122–128

    Article  Google Scholar 

  • Zhen YD (2018) Control of damping-off in red pine seedlings. Protec For Sci Tech 174:90–91

    Google Scholar 

Download references

Acknowledgements

This work was supported by the Introduction of Talent Project of Guizhou University ([2015]67) and the Science and Technology Support Projects of Guizhou Province (Qiankehe Support [2018]2375-2; Qiankehe Platform Talent [2018]5781; Qiankehe Platform Talent [2018]5261; Qiankehe Foundation [2017]1058).

Author information

Authors and Affiliations

  1. College of Forestry, Guizhou University, Huaxi District, Guiyang, 550025, China

    Xin Luo & Cun Yu

Authors
  1. Xin Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cun Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cun Yu.

Ethics declarations

Conflict of interest

All authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Luo, X., Yu, C. First report of damping-off disease caused by Fusarium oxysporum in Pinus massoniana in China. J Plant Dis Prot 127, 401–409 (2020). https://doi.org/10.1007/s41348-020-00303-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s41348-020-00303-3

Keywords

Search

Navigation