Abstract
In 2020, a canker disease with dieback of branches and decline of various fig trees in Şanlıurfa and Mardin provinces of Turkey was observed. The causal pathogen was identified as Neoscytalidium dimidiatum based on morphological characteristics and phylogenetic analysis of ITS, tef1, and tub2 loci. Koch’s postulates were confirmed by successful re-isolation of N. dimidiatum only from plants inoculated with the pathogen. This is the first report of N. dimidiatum associated with dieback and canker of common fig in Turkey.
References
Aiello D, Gusella G, Fiorenza A, Guarnaccia V, Polizzi G (2020) Identification of Neofusicoccum parvum causing canker and twig blight on Ficus carica in Italy. Phytopathol Mediterr 59:147–153. https://doi.org/10.14601/Phyto-10798
Akın M (2021) SSR based molecular characterization of local fig (Ficus carica L.) Germplasm in Northeastern Turkey. Erwerbs-Obstbau 63(4):387–392. https://doi.org/10.1007/s10341-021-00596-0
Banihashemi Z, Javadi AR (2009) Further investigations on the biology of Phomopsis cinerascens, the cause of fig canker in Iran. Phytopathol Mediterr 48:454–460. https://doi.org/10.14601/Phytopathol_Mediterr-2947
Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556. https://doi.org/10.1080/00275514.1999.12061051
Crous PW, Slippers B, Wingfield MJ, Rheeder J, Marasas WFO (2006) Phylogenetic lineages in the Botryosphaeriaceae. Stud Mycol 55:235–253. https://doi.org/10.3114/sim.2011.69.01
Çeliker NM, Michailides TJ (2012) First report of Lasiodiplodia theobromae causing canker and shoot blight of fig in Turkey. New Dis Rep 25:12. https://doi.org/10.5197/j.2044-0588.2012.025.012
Chen Y, Wei H, Du G, Zhu L, Song Q, Hu Y, Wang E, Wang M, Fan X (2018) First report of Lasiodiplodia theobromae causing stem canker on common fig (Ficus carica) in Zhejiang Province of China. Plant Dis 102:2656–2657. https://doi.org/10.1094/PDIS-05-18-0887-PDN
Derviş S, Türkölmez Ş, Çiftçi O, Serçe ÇU, Dikilitas M (2019a) First report of Neoscytalidium dimidiatum causing canker, shoot blight, and root rot of pistachio in Turkey. Plant Dis 103:1411–1411. https://doi.org/10.1094/PDIS-01-19-0053-PDN
Derviş S, Türkölmez Ş, Çiftçi O, Ulubaş Serçe Ç, Dikilitas M (2019b) First report of Neoscytalidium dimidiatum causing black canker and root rot of walnut in Turkey. Plant Dis 103(8):2129–2129. https://doi.org/10.1094/PDIS-02-19-0306-PDN
Elshafie AE, Ba-Omar T (2002) First report of Albizia lebbeck dieback caused by Scytalidium dimidiatum in Oman. Mycopathologia 154:37–40. https://doi.org/10.1023/A:1015200707971
Farr DF, Rossman AY (2021) Fungal Databases, U.S. National Fungus Collections, ARS, USDA. https://nt.ars-grin.gov/fungaldatabases/. Accessed 14 December 2021
FAOSTAT D (2021) Food and agriculture organization of the United Nations. Statistical database. http://www.fao.org/faostat/en/#data/QC. Accessed 18 December 2021
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
Ferguson L, Michailides TJ, Shorey HH (1990) The California Fig Industry. Hortic Rev 12:409–490. https://doi.org/10.1002/9781118060858.ch9
Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Env Microbiol 61:1323–1330. https://doi.org/10.1128/AEM.61.4.1323-1330.1995
Güney İG, Özer G, Turan İ, Koşar İ, Derviş S (2021) First report of Neoscytalidium dimidiatum causing foliar and stem blight of lavender in Turkey. J Plant Pathol 103:1347–1348. https://doi.org/10.1007/s42161-021-00917-y
Gusella G, Morgan DP, Michailides TJ (2021) Further Investigation on Limb Dieback of Fig (Ficus carica) Caused by Neoscytalidium dimidiatum in California. Plant Dis 105(2):324–330. https://doi.org/10.1094/PDIS-06-20-1226-RE
Huang SK, Tangthirasunun N, Phillips AJL, Dai DQ, Wanasinghe DN (2016) Morphology and phylogeny of Neoscytalidium orchidacearum sp nov (Botryosphaeriaceae). Mycobiology 44:79–84. https://doi.org/10.5941/MYCO.2016.44.2.79
Kajitani Y, Masuya H (2011) Ceratocystis ficicola sp. nov., a causal fungus of fig canker in Japan. Mycoscience 52:349–353. https://doi.org/10.1007/S10267-011-0116-5
Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief Bioinform 20:1160–1166. https://doi.org/10.1093/bib/bbx108
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096
Oksal E, Çelik Y, Özer G (2019) Neoscytalidium dimidiatum causes canker and dieback on grapevine in Turkey. Australas Plant Dis Notes 14(1):1–3. https://doi.org/10.1007/s13314-019-0363-4
Oksal E, Yiğit T, Özer G (2020) First report of Neoscytalidium dimidiatum causing shoot blight, dieback and canker of apricot in Turkey. J Plant Pathol 102:579–580. https://doi.org/10.1007/s42161-019-00467-4
Ören E, Palacıoğlu G, Koca G, Ozan GN, Bayraktar H (2021) First report of Neoscytalidium dimidiatum causing branch dieback and canker on apple in Turkey. J Plant Pathol. https://doi.org/10.1007/s42161-021-00990-3
Patil VV, Patil VR (2011) Ficus carica Linn.-an overwiev. J Med Plants Res 5:246–253
Phillips AJ, Alves A, Abdollahzadeh J, Slippers B, Wingfield MJ, Groenewald JZ, Crous PW (2013) The Botryosphaeriaceae: genera and species known from culture. Stud Mycol 76:51–167. https://doi.org/10.3114/sim0021
Ray JD, Burgess T, Lanoiselet VM (2010) First record of Neoscytalidium dimidiatum and N. novaehollandiae on Mangifera indica and N. dimidiatum on Ficus carica in Australia. Australas Plant Dis Notes 5:48–50. https://doi.org/10.1071/DN10018
Solomon A, Golubowicz S, Yablowicz Z, Grossman S, Bergman M, Gottlieb HE, Flaishman MA (2006) Antioxidant activities and anthocyanin content of fresh fruits of common fig (Ficus carica L.). J Agric Food Chem 54(20):7717–7723. https://doi.org/10.1021/jf060497h
Teoman S, Ipek M, Erturk U, Tangu NA, Durgut E, Barut E, Ipek A (2017) Assessment of genetic relationship among male and female fig genotypes using simple sequence repeat (SSR) markers. Not Bot Horti Agrobot Cluj Napoca 45(1):172–178. https://doi.org/10.15835/nbha45110756
Tsopelas P (2021) Ceratocystis ficicola causing a serious disease of Ficus carica in Greece. Phytopathol Mediterr 60:337–349. https://doi.org/10.36253/phyto-12794
Türkölmez Ş, Derviş S, Çiftçi O, Serçe ÇU, Dikilitas M (2019a) New disease caused by Neoscytalidium dimidiatum devastates tomatoes (Solanum lycopersicum) in Turkey. Crop Prot 118:21–30. https://doi.org/10.1016/j.cropro.2018.12.004
Türkölmez Ş, Derviş S, Çiftçi O, Dikilitas M (2019b) First report of Neoscytalidium dimidiatum causing shoot and needle blight of pines (Pinus spp.) in Turkey. Plant Dis 103:2960–2961. https://doi.org/10.1094/PDIS-05-19-0964-PDN
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols, Guide to Methods and Applications, Academic Press, San Diego, pp 315−322. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
Zhang W, Groenewald JZ, Lombard L, Schumacher RK, Phillips AJL, Crous PW (2020) Evaluating species in Botryosphaeriales. Persoonia 46:63–115. https://doi.org/10.3767/persoonia.2021.46.03
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Güney, İ.G., Bozoğlu, T., Özer, G. et al. First report of Neoscytalidium dimidiatum associated with dieback and canker of common fig (Ficus carica L.) in Turkey. J Plant Dis Prot 129, 701–705 (2022). https://doi.org/10.1007/s41348-022-00586-8
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DOI: https://doi.org/10.1007/s41348-022-00586-8