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Understanding components of the grapevine leaf spot monocycle and comparing resistance of Vitis labrusca cultivars

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Abstract

Grapevine leaf spot caused by Pseudocercospora vitis is important at the end of the vegetative cycle of Vitis labrusca and its hybrids, but studies on this pathosystem and its monocyclic components are scarce. The objective of this work was to determine the favorable conditions for the development of the pathogen and to study the resistance levels in 10 grapevine cultivars. The germination of P. vitis conidia was evaluated at temperatures from 4 to 35 °C, germ tube size from 8 to 33 °C and weight of colonies of the pathogen from 10 to 40 °C. Wetness periods of 3 to 48 h were also evaluated for germination at temperatures from 10 to 40 °C. In five cultivars of V. labrusca and five hybrids inoculated with P. vitis, the incubation period and the progression of disease severity under controlled conditions were compared. The optimal temperature for germination of P. vitis was at 28 °C and 27 °C for germ tube growth. For mycelial weight the optimum temperature was 26.4 °C. The highest germination of the pathogen occurred with wetness of 45.5 h and temperature of 29.6 °C. The incubation period in the cultivars ranged from 26 to 51 days. The disease progress curve showed differences among cultivars tested, ‘Concord’ and ‘BRS Vitória’ being the most resistant. In plants inoculated at 25 °C, the number of lesions increased over time, stabilizing at 42–47 days after inoculation and from here no new lesions appeared, only lesions expansion was observed.

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Acknowledgements

The authors would like to thank the ‘Federal University of Parana (UFPR)’, as well as the ‘Coordination of Improvement of Higher Education Personnel (CAPES)’, for their support and provision of resources necessary for our research.

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Authors and Affiliations

  1. Setor de Ciencias Agrárias, Departamento de Fitotecnia e Fitossanidade, Universidade Federal do Paraná (UFPR), Curitiba, PR, 80035-050, Brazil

    Eliane Aparecida Rogovski-Czaja, Rafaele Regina Moreira, Henrique da Silva Silveira Duarte & Louise Larissa May De Mio

  2. Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (EPAGRI), Chapecó, SC, 89801970, Brazil

    Cristiano Nunes Nesi

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  1. Eliane Aparecida Rogovski-Czaja
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  2. Rafaele Regina Moreira
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  3. Cristiano Nunes Nesi
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  4. Henrique da Silva Silveira Duarte
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  5. Louise Larissa May De Mio
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Correspondence to Louise Larissa May De Mio.

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Rogovski-Czaja, E.A., Moreira, R.R., Nesi, C.N. et al. Understanding components of the grapevine leaf spot monocycle and comparing resistance of Vitis labrusca cultivars. J Plant Pathol 101, 897–906 (2019). https://doi.org/10.1007/s42161-019-00281-y

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  • DOI: https://doi.org/10.1007/s42161-019-00281-y

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