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Exploring the taxonomic classification of Curvularia genera: Enhancing understanding of phytopathogenic species in Poaceae through morphological and molecular approaches

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Abstract

The genus Curvularia consists of important plant pathogens typically associated with major cereals such as rice, wheat, maize, millets and others and poses a threat to the security of the global food supply. In this study, morphological characteristics of fifty-two Curvularia isolates were analyzed, resulting in the segregation of the isolates into 16 distinct groups based on their morphological traits. The study entailed an in-depth characterization of a range of Curvularia species, including Curvularia aeria, Curvularia affinis, Curvularia australiensis, Curvularia borreriae, Curvularia catenulata, Curvularia clavata, Curvularia eragrostidis, Curvularia geniculata, Curvularia inaequalis, Curvularia lunata, Curvularia pallescens, Curvularia prasadii, Curvularia spicifera, Curvularia trifoli, Curvularia tuberculata, and Curvularia verruculosa. The study includes comprehensive descriptions of their colony appearances, conidiophore structures, and conidial characteristics. Furthermore, a phylogenetic analysis utilizing ITS, LSU, and SSU gene sequences provides valuable insights into the genetic relationships among these isolates.

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Data availability

All data that support the findings of the present study are included in the manuscript. The ITS, SSU and LSU nucleotide sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov.

References

  • Ahmadpour A, Heidarian Z, Donyadoost-Chelan M, Javan-Nikkhah M, Tsukiboshi T (2012) A new species of Bipolaris from Iran. Mycotaxon 120:301–307

    Article  Google Scholar 

  • Berbee ML, Pirseyedi M, Hubbard S (1999) Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia 91:964–977

    Article  CAS  Google Scholar 

  • Bhunjun CS, Dong Y, Jayawardena RS, Jeewon R, Phukhamsakda C, Bundhun D, Hyde KD, Sheng J (2020) A polyphasic approach to delineate species in Bipolaris. Fungal Divers 102:225–256

    Article  Google Scholar 

  • Boedijn KB (1933) Bulletin du Jardin Botanique de Buitenzorg. 3 Sér 13:123

  • Castellani E (1951) Una nuova specie di Cochliobolus. Mycopathologia 6:51–57

    Google Scholar 

  • Choi YW, Hyde KD, Ho WH (1999) Single spore isolation of fungi. Fungal Divers 3:29–38

    Google Scholar 

  • Chomnunti P, Schoch CL, Aguirre-Hudson B, Ko-Ko TW, Hongsanan S, Jones EG, Kodsueb R, Phookamsak R, Chukeatirote E, Bahkali AH, Hyde KD (2011) Capnodiaceae Fungal Divers 51:103–134

    Article  PubMed  Google Scholar 

  • Culling KW (1993) Design and testing of plant specific PCR primer for ecological evolutionary studies. Mol Ecol 1:233–240

    Article  Google Scholar 

  • de Hoog GS, Zalar P, Gerrits van den Ende AHG, Gunde-Cimerman N (2005) Relation of halotolerance to human-pathogenicity in the fungal tree of life: An overview of ecology and evolution under stress. Adaptation to Life at High Salt Concentrations in Archaea, Bacteria, and Eukarya. Springer, Dordrecht, The Netherlands

  • Drechsler C (1934) Phytopathological and taxonomical aspects of Ophilobolus, Pyrenophora, Helminthosporium and a new genus Cochliobolus. Phytopathol 24:973

    Google Scholar 

  • Ellis MB (1971) Dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew, p 608

  • Emami K, Hack E (2002) Conservation of XYN11A and XYN11B xylanase genes in Bipolaris sorghicola, Cochliobolus sativus, Cochliobolus heterostrophus, and Cochliobolus spicifer. Curr Microbiol 45:303–306

    Article  CAS  PubMed  Google Scholar 

  • Goh TK, Hyde KD, Lee DKL (1998) Generic distinction in the Helminthosporium complex based on restriction analysis of the nuclear ribosomal RNA gene. Fungal Diversity 1:85–107

    Google Scholar 

  • Jeevan B, Gogoi R, Sharma D, Manjunatha C, Rajashekara H, Ram D, Mishra KK, Mallikarjuna MG (2020) Genetic analysis of maydis leaf blight resistance in subtropical maize (Zea mays L.) germplasm. J Genet 99:1–9

    Article  Google Scholar 

  • Kashyap AS, Manzar N, Maurya A, Mishra DD, Singh RP, Sharma PK (2023a) Development of Diagnostic Markers and Applied for Genetic Diversity Study and Population Structure of Bipolaris sorokiniana Associated with Leaf Blight Complex of Wheat. J Fungi 9:153

    Article  CAS  Google Scholar 

  • Kashyap AS, Manzar N, Meshram S, Sharma PK (2023b) Screening microbial inoculants and their interventions for cross kingdom management of wilt disease of solanaceous crop- A step towards sustainable agriculture. Front Microbiol 14. https://doi.org/10.3389/fmicb.2023.1174532

  • Kashyap AS, Manzar N, Nebapure SM, Rajawat MVS, Deo MM, Singh JP, Kesharwani AK, Singh RP, Dubey SC, Singh D (2022) Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt Disease. Antioxidants 11:404

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Krizsan K, Papp T, Manikandan P, Shobana CS, Chandrasekaran M, Vagvolgyi C, Kredics L (2016) Clinical Importance of the Genus Curvularia. Medical Mycology: Current Trends and Future Prospects 147–204

  • Kusai NA, Madihah MZA, Shahrizim Z, Mohd TY, Nur Ain Izzati MZ (2015) Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia. Rend Fis Acc Lincei 27:205–214

    Article  Google Scholar 

  • Luttrell ES (1963) Taxonomic criteria in Helminthosporium. Mycologia 55:643–674

    Google Scholar 

  • Madrid H, da Cunha KC, Gené J, Dijksterhuis J, Cano J, Sutton DA, Guarro J, Crous PW (2014) Novel Curvularia species from clinical specimens. Persoonia 33:48–60

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Manamgoda DS, Cai L, Bahkali AH, Chukeatirote E, Hyde KD (2011) Cochliobolus: An overview and current status of species. Fungal Divers 51:3–42

    Article  Google Scholar 

  • Manamgoda DS, Rossman AY, Castlebury LA, Crous PW, Madrid H, Chukeatirote E, Hyde KD (2014) The genus Bipolaris. Stud Mycol 79:221–288

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Manzar N, Kashyap AS, Maurya A, Rajawat MVS, Sharma PK, Srivastava AK, Roy M, Saxena AK, Singh HV (2022) Multi-Gene Phylogenetic Approach for Identification and Diversity Analysis of Bipolaris maydis and Curvularia lunata Isolates Causing Foliar Blight of Zea mays. J Fungi 8:802

    Article  CAS  Google Scholar 

  • Manzar N, Kashyap AS, Sharma PK, Saxena AK (2021) First Report of Leaf Spot of Maize Caused by Curvularia geniculata in India. Plant Dis. https://doi.org/10.1094/PDIS-03-21-0637-PDN

    Article  Google Scholar 

  • Marin-Felix Y, Groenewald JZ, Cai L, Chen Q, Marincowitz S, Barnes I, Bensch K, Braun U, Camporesi E, Damm U, De Beer ZW (2017) Genera of phytopathogenic fungi: GOPHY 1. Stud Mycol 86:99–216

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • McNeill J, Barrie FR, Buck WR, Demoulin V, Greuter W, Hawksworth DL, Herendeen PS, Knapp S, Marhold K, Prado J, Prud’homme van Reine WF, Smith GF, Wiersema J, Turland NJ (eds) (2012) International Code of Nomenclature for algae, fungi and plants (Melbourne Code). Königstein: Koeltz Scientific Books. Vol. 154, p 240

  • Mendoza L, Ajello L, Taylor JW (2001) The taxonomic status of Lacazia loboi and Rhinosporidium seeberi has been finally resolved with the use of molecular tools. Rev Iberroam Micol 18:95–98

    CAS  Google Scholar 

  • Ramesh GV, Palanna KB, Vinaykumar HD, Kumar A, Koti PS, Mahesha HS, Nagaraja TE, Tonapi VA, Jeevan B (2021) Occurrence and characterization of Bipolaris setariae associated with leaf blight of browntop millet (Brachiaria ramosa) in India. J Phytopathol 00:1–10

    Google Scholar 

  • Rinaldi MG, Phillips P, Schwartz JG, Winn RE, Holt GR, Shagets FW, Elrod J, Nishioka G, Aufdemorte TB (1987) Human Curvularia infections. Report of five cases and review of the literature. Diagn Microbiol Infect Dis 6:27–39

  • Shoemaker RA (1959) Nomenclature of Drechslera and Bipolaris, grass parasites segregated from ’Helminthosporium’. Canad J Bot 37:879–887

    Article  Google Scholar 

  • Sivanesan A (1987) Graminicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and Their Teleomorphs. Mycol Pap 158:1–261

    Google Scholar 

  • Subramanian CV, Jain BL (1966) A revision of some graminicolus Helminthospora. Curr Sci 35:352–355

    Google Scholar 

  • Sun X, Qi X, Wang W, Liu X, Zhao H, Wu C, Chang X, Zhang M, Chen H, Gong G (2020) Etiology and symptoms of maize leaf spot caused by Bipolaris spp. in Sichuan, China. Pathogens 9:229

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

  • Tan YP, Madrid H, Crous PW, Shivas RG (2014) Johnalcornia gen. et. comb. nov., and nine new combinations in Curvularia based on molecular phylogenetic analysis. Australas Plant Pathol 43:589–603

  • Tsuda M, Ueyama A, Nishih N (1978) Pseudocochliobolus lunatus (R.R. Nelson & F.A. Haasis) the perfect stage of Helminthosporium coicis. Mycologia 69:1118

  • Valente P, Ramos J, Leoncini O (1999) Sequencing as a tool in yeast molecular taxonomy. Can J Microbiol 45:949–958

    Article  CAS  PubMed  Google Scholar 

  • Wakker DZ (1898) het Suikerriet op Java: 196

Download references

Acknowledgements

The authors are thankful to the Head, Division of Plant Pathology, ICAR—Indian Agricultural Research Institute, New Delhi for providing laboratory and financial support. The first author is grateful to the University grants commission (UGC), for awarding Rajiv Gandhi National Fellowship (RGNF) for Ph.D. program.

Funding

The present research was funded by the Division of Plant Pathology, ICAR—Indian Agricultural Research Institute, New Delhi and also the University grants commission (UGC) via the award of Rajiv Gandhi National Fellowship (RGNF) for a Ph.D. program. The research is a part of PhD thesis of Dr. Dama Ram.

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Authors and Affiliations

  1. Agriculture University, Jodhpur, Rajasthan, India

    Dama Ram

  2. ICAR-Indian Agricultural Research Institute, New Delhi, India

    Thokala Prameela Devi & Deeba Kamil

  3. The University of Trans-Disciplinary Health Sciences and Technology, Bengaluru, India

    Prasanna S. Koti

  4. ICAR- National Rice Research Institute, Cuttack, Odisha, India

    Bettanayaka Jeevan & Shivappa Raghu

  5. Regional Agricultural Research Station, Anakapalle, Acharya N.G. Ranga Agricultural University, Amaravathi Rd, Lam, Guntur, Andhra Pradesh, 522034, India

    Chandra Sekhar Vanapalli

  6. ICAR-Indian Institute of Pulse Research (RS), Bhubaneswar, 752055, India

    Sunil Kumar Sunani

  7. ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath, Bhanjan, 275103, India

    Abhijeet Shankar Kashyap

Authors
  1. Dama Ram
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  2. Thokala Prameela Devi
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  4. Bettanayaka Jeevan
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Contributions

“Conceptualization, D.R., D.K. and T.P.D.; methodology, D.R., J.B. and C.S.V.; software, D.R., J.B., P.S.K., D.K.; validation, D.R., J.B. and C.S.V.; formal analysis, P.S.K., J.B., A.S.K., R.S., and S.K.S.,; investigation, D.R., J.B. and C.S.V.; resources, D.K. and T.P.D.; data curation, D.R., J.B., P.S.K., A.S.K., R.S., M.S.R; writing—original draft preparation, D.R., J.B. P.S.K., and A.S.K.; writing—review and editing, D.R., J.B., P.S.K., T.P.D., C.S.V., A.S.K., R.S., and S.K.S.; supervision, T.P.D., D.K. and J.B.; project administration, D.K. and T.P.D.; funding acquisition, D.R. and T.P.D. All authors read and approved the final version of the manuscript.”

Corresponding authors

Correspondence to Bettanayaka Jeevan or Abhijeet Shankar Kashyap.

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Ram, D., Devi, T.P., Koti, P.S. et al. Exploring the taxonomic classification of Curvularia genera: Enhancing understanding of phytopathogenic species in Poaceae through morphological and molecular approaches. J Plant Pathol (2024). https://doi.org/10.1007/s42161-023-01560-5

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