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Characterization and genetic diversity of Pantoea agglomerans isolates having dual potentiality to suppress growth of Ralstonia solanacearum and plant growth promoting ability

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Abstract

Twenty six isolates of genus Pantoea collected from leaves of rice and carrot (Experimental Farm, IARI, New Delhi), potato tuber (Hapur, Uttar Pradesh) and tomato leaves (Bengaluru, Karnataka) were characterized as Pantoea agglomerans by using morphological, physiological, biochemical methods and 16S rRNA nucleotide sequence analysis. Twenty six isolates of P. agglomerans, two reference strains of P. agglomerans (DWM-1and DWM-2) and three out group bacteria viz., Bacillus subtilis DTBS-5, B. cereus JHTBS-7, and Pseudomonas fluorescens DTPF-3 were taken to study genetic diversity by using BOX–PCR primers. The BOX primer produced 8–26 bands with mol. size 0.25 kb to 10 kb. DRP-16 and UPP-21 isolates had maximum number of fragments (15 fragments) followed by DRP-11, DRP-13 and DRP-14, UPP-18, UPP-19. Fifteen major groups at 75% similarity coefficient were obtained and high similarities were found among genetic profile of Delhi and Uttar Pradesh isolated strains, whereas the strains of Karnataka state separated in other groups. Out of 28 isolates of P. agglomerans, 12 showed antagonistic property against R. solanacearum in vitro conditions. Isolate DRP-7 showed highest inhibition zone (2.8 cm2) against pathogenic bacterium (R. solanacearum) followed by 2.5 cm2 in DRP-8 and UPP-20. Plant growth promoting activities of P. agglomerans isolates were tested in vitro situation. Majority of the isolates of P. agglomerans had good potentiality to solubilize the phosphorus, produce siderophores, indole acetic acid and ammonia. The result showed that the isolates DRP-7 and UPP-20 have both antagonistic and growth promoting ability, which is a good candidate to manage wilt disease and promote growth of tomato plants.

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Acknowledgements

The authors are grateful to Dr. Rashmi Aggarwal, Head, Division of Plant Pathology, ICAR- Indian Agricultural Research Institute, New Delhi for her continuous support and encouragement for conducting the experiments.

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  1. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Dinesh Singh & D. K. Yadav

  2. Department of Biotechnology, GLA University, Mathura, Uttar Pradesh, India

    Farhat Fatima

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  1. Dinesh Singh
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Singh, D., Yadav, D.K. & Fatima, F. Characterization and genetic diversity of Pantoea agglomerans isolates having dual potentiality to suppress growth of Ralstonia solanacearum and plant growth promoting ability. Indian Phytopathology 73, 643–653 (2020). https://doi.org/10.1007/s42360-020-00268-1

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