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Dark septate endophytic fungi increase the activity of proton pumps, efficiency of 15N recovery from ammonium sulphate, N content, and micronutrient levels in rice plants

  • Food Microbiology - Research Paper
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Abstract

Plants colonised by dark septate endophytic (DSE) fungi show increased uptake of nutrients available in the environment. The objective of the present study was to evaluate the impact of DSE fungi on the activity of proton pumps, nitrogen (N) recovery from ammonium sulphate, and nutrient accumulation in rice plants. Treatments consisted of non-inoculated plants and plants inoculated with two isolates of DSE fungi, A101 and A103. To determine N recovery from the soil, ammonium sulphate enriched with 15N was added to a non-sterile substrate while parameters associated with the activity of proton pumps and with NO3− uptake were determined in a sterile environment. The A101 and A103 fungal isolates colonised the roots of rice plants, promoting 15N uptake, growth, and accumulation of nutrients as compared with the mock control. A103 induced the expression of the plasma membrane H+-ATPase (PM H+-ATPase) isoforms OsA5 and OsA8, the activity of the PM H+-ATPase and H+-pyrophosphatase. Our results suggest that the inoculation of rice plants with DSE fungi represents a strategy to improve the N recovery from ammonium sulphate and rice plant growth through the induction of OsA5 and OsA8 isoforms and stimulation of the PM H+-ATPase and H+-pyrophosphatase.

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Acknowledgements

We are indebted to the University Federal Rural do Rio de Janeiro (UFRRJ), especially for the Laboratory Nutrition of Plants, the state University of Norte Fluminense Darcy Ribeiro (UENF), especially for LBCT, the Brazilian Agricultural Research Corporation (Embrapa), for their help with the work. This work was supported by the Foundation for Support of Research in the State of Rio de Janeiro (FAPERJ), the Brazilian National Council for Scientific and Technological Development (CNPq), and the Coordination for the Improvement of Higher Education Personnel (CAPES).

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  1. Universidade Federal Rural do Rio de Janeiro, Instituto de Agronomia, Seropédica, RJ, Brazil

    Carlos Vergara, Karla Emanuelle Campos Araujo & Leandro Azevedo Santos

  2. Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil

    Marcus Vinícius Loss Sperandio

  3. Embrapa Agrobiologia, BR 465, km 07, Seropédica, RJ, 23891-000, Brazil

    Segundo Urquiaga & Jerri Édson Zilli

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Vergara, C., Araujo, K.E.C., Sperandio, M.V.L. et al. Dark septate endophytic fungi increase the activity of proton pumps, efficiency of 15N recovery from ammonium sulphate, N content, and micronutrient levels in rice plants. Braz J Microbiol 50, 825–838 (2019). https://doi.org/10.1007/s42770-019-00092-4

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