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Exploring the mycobiota of bromeliads phytotelmata in Brazilian Campos Rupestres

  • Environmental Microbiology - Research Paper
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Abstract

The phytotelmata is a water-filled tank on a terrestrial plant, and it plays an important role in bromeliad growth and ecosystem functioning. Even though previous studies have contributed to elucidate the composition of the prokaryotic component of this aquatic ecosystem, its mycobiota (fungal community) is still poorly known. In the present work, ITS2 amplicon deep sequencing was used to examine the fungal communities inhabiting the phytotelmata of two bromeliads species that coexist in a sun-exposed rupestrian field of Southeastern Brazil, namely Aechmea nudicaulis (AN) and Vriesea minarum (VM). Ascomycota was the most abundant phylum in both bromeliads (57.1 and 89.1% in AN and VM respectively, on average), while the others were present in low abundance (< 2%). Mortierellomycota and Glomeromycota were exclusively observed in AN. Beta-diversity analysis showed that samples from each bromeliad significantly clustered together. In conclusion, despite the considerable within-group variation, the results suggested that each bromeliad harbor a distinct fungi community, what could be associated with the physicochemical characteristics of the phytotelmata (mainly total nitrogen, total organic carbon, and total carbon) and plant morphological features.

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Data availability

The ecological datasets in the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors are grateful to Department of Microbiology of Universidade Federal de Minas Gerais and Department of Biological Sciences of Centro Federal de Educação Tecnológica de Minas Gerais for research facilities. The authors are also thankful for the support from the agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) grant no. CBB-APQ-02639-15.

Funding

This research was supported by Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) grant no. CBB-APQ-02639-15.

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Authors and Affiliations

  1. Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil

    Vera Lúcia dos Santos, Ubiana Cássia Silva, Eduardo Horta Santos & Marcela França Dias

  2. Expertise Center Botany and Biodiversity, Museu de História Natural e Jardim Botânico, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31080-010, Brazil

    Alessandra Abrão Resende

  3. Universidad Católica del Maule, Facultad de Ciencias Agrarias y Forestales, Centro de Biotecnología de los Recursos Naturales, 3480112, Talca, Chile

    Sara Cuadros-Orellana

  4. Department of Biological Sciences, Centro Federal de Educação Tecnológica de Minas Gerais – CEFET/MG, Av. Amazonas, 5253, Nova Suíça, 30.421-169, Belo Horizonte, MG, 30421-169, Brazil

    Andréa Rodrigues Marques

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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analyses were performed by Ubiana Cássia Silva, Andrea R. Marques, Alessandra Abrão Resende, and Vera Lúcia dos Santos. Writing of original draft, Vera Lúcia dos Santos. Writing and review editing, data analysis, and discussion were carried out by Sara Cuadros-Orellana, Andrea R. Marques, Alessandra Abrão Resende, Marcela França Dias, and Vera Lúcia dos Santos. All authors read and approved the final manuscript.

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Correspondence to Vera Lúcia dos Santos.

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Permissions for collection according to ICMBio instructions. Document 57088-1 was registered by AA Resende in SISBIO and document 097/2015 was registered by AA Resende in IEF/MG as authorization for collection of phytotelmata water and seed samples for scientific activities.

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dos Santos, V.L., Silva, U.C., Santos, E.H. et al. Exploring the mycobiota of bromeliads phytotelmata in Brazilian Campos Rupestres. Braz J Microbiol 54, 1885–1897 (2023). https://doi.org/10.1007/s42770-023-00977-5

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