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Sensing Inside the Living Cells

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Introduction to Fluorescence Sensing

Abstract

The full power of fluorescence technique can be demonstrated in imaging and sensing within living cells. Its most important advantage that will probably be never competed by any other technique is a high spatial resolution together with ultimate contrast and sensitivity obtained in a noninvasive or low invasive manner. Exploration of these possibilities has led to development of powerful methods of fluorescence microscopy, such as confocal, two-photon and evanescent-wave microscopy. Resolution in time and anisotropy were successfully applied to microscopy. Recent advances allowed achieving superresolution images and observing individual molecules. All these developments lead to much deeper understanding the cell functioning, the dynamics of interactions and transformations of its constituents and metabolites. The tools for achieving that are overviewed in this Chapter, critically discussing their advantages and limitations. At the end, the reader finds the section “Sensing and thinking” with the questions and problems.

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  1. Laboratory of Nanobiotechnology Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

    Alexander P. Demchenko

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Demchenko, A.P. (2015). Sensing Inside the Living Cells. In: Introduction to Fluorescence Sensing. Springer, Cham. https://doi.org/10.1007/978-3-319-20780-3_13

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