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Strategies for Screening Induced Mutants for Stress Tolerance

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Mutation Breeding for Sustainable Food Production and Climate Resilience

Abstract

Crop plants are exposed to a multitude of biotic and abiotic factors, which necessitates improving tolerance/resistance in plant germplasm using conventional, mutagenesis and biotechnological approaches. Several induced mutants have been improved for tolerance/resistance with other desirable characteristics and mutant varieties have been released for cultivation. As per the FAO/IAEA mutant variety database, so far 248 and 558 mutant varieties have been developed for biotic and abiotic stress tolerance, respectively. Progress in this area has been majorly based on mutation induction and screening of putative mutants using a wide range of selection strategies especially, pre-field screening at seedling and flowering stages based on simple phenotypic responses. Several methodological considerations are now advocated for taking up a suitable in vivo/in vitro screening method for a given plant stress factor. For a specific crop and stress tolerance, it is necessary to establish reliable, rapid and high throughput screening techniques in putative mutant lines. Plant cell and tissue culture based in vitro selection strategies have been established for developing stress-tolerant mutants using selection agents for biotic stress, for example, culture filtrate, pathotoxin, or spore suspension or NaCl or PEG. Disease-resistant lines have been developed in banana, carnation, grapevine, strawberry, pineapple, tomato, alfalfa, barley, chickpea, asparagus, sugarcane, celery, oats and wheat for use in breeding programmes. Methodologies to screen for the mutant traits using different cellular, analytical and molecular tools will have to be extensively adopted in plant populations under stress conditions.

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Authors and Affiliations

  1. Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency Vienna, Vienna, Austria

    Fatma Sarsu

  2. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India

    Suprasanna Penna

  3. Seva Sadan’s R.K. Talreja College of Arts, Science and Commerce, Mumbai, India

    Ganesh C. Nikalje

  4. Amity Centre for Nuclear Biotechnology & Amity Institute of Biotechnology, Amity University, Mumbai, Maharashtra, India

    Suprasanna Penna

Authors
  1. Fatma Sarsu
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  2. Suprasanna Penna
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  3. Ganesh C. Nikalje
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Editor information

Editors and Affiliations

  1. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India

    Suprasanna Penna

  2. Department Agricultural Sciences, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

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© 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

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Sarsu, F., Penna, S., Nikalje, G.C. (2023). Strategies for Screening Induced Mutants for Stress Tolerance. In: Penna, S., Jain, S.M. (eds) Mutation Breeding for Sustainable Food Production and Climate Resilience. Springer, Singapore. https://doi.org/10.1007/978-981-16-9720-3_6

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