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Diagnosis and Detection of Soil-Borne Fungal Phytopathogens in Major Crops

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Detection, Diagnosis and Management of Soil-borne Phytopathogens

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Abstract

Phytopathogenic soil-borne fungal species can inflict huge economic disturbances in the global agricultural sector. Soil-borne diseases, incited by fungal pathogens, e.g. root rot, stem rot, crown rot, damping-off, blights, vascular wilts, etc., inflict significant economic losses in agricultural and horticultural crops’ yields and quality, globally. To achieve effective disease control, precise and quick detection or identification of plant infecting fungi is required. For accurate plant disease diagnosis, DNA-based approaches have become widespread. Recent breakthroughs in the field of fungal detection and differentiation; various polymerase chain reaction (PCR) assays such as nested, multiplex, quantitative, bio, and magnetic-capture hybridisation PCR techniques; post and isothermal amplification methods; DNA and RNA-based probe development; and next-generation sequencing have resulted in novel molecular diagnostic tools. Symptomatic and asymptomatic diseases caused by culturable and non-culturable fungal pathogens can be detected using these molecular-based detection approaches in both single-infection and co-infection conditions. Plant disease diagnostics require molecular techniques that are more reliable, quicker, and easier to use than traditional procedures. The present chapter highlights molecular diagnostic tools that have come a long way including rapid developments in recent past. However, it requires further firming up before becoming integral part of efficient plant disease management.

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Authors and Affiliations

  1. ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, India

    Ravindra Kumar, Sudheer Kumar, Ankush Kumar, Aradhana Sagwal, Ratan Tiwari & G. P. Singh

  2. School of Agriculture, Lovely Professional University, Phagwara, Punjab, India

    Seweta Srivastava & Tsewang Chosdon

  3. Plant-Microbe Interaction and Rhizosphere Biology Lab, ICAR-National Bureau of Agriculturally Important Microorganisms, Kushmaur, Maunath Bhanjan, Uttar Pradesh, India

    Udai B. Singh

  4. ICAR-Indian Agricultural Research Institute, Regional Station, Karnal, Haryana, India

    Manoj Kumar Yadav

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  1. Ravindra Kumar
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  1. ICAR-National Bureau of Agriculturally Important Microorganisms, Maunath Bhanjan, Uttar Pradesh, India

    Udai B. Singh

  2. Division of Crop Protection, ICAR-Indian Institute of Wheat and Barley Research, Karnal, Haryana, India

    Ravindra Kumar

  3. Department of Biotechnology, GLA University, Mathura, Uttar Pradesh, India

    Harikesh Bahadur Singh

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Kumar, R. et al. (2023). Diagnosis and Detection of Soil-Borne Fungal Phytopathogens in Major Crops. In: Singh, U.B., Kumar, R., Singh, H.B. (eds) Detection, Diagnosis and Management of Soil-borne Phytopathogens. Springer, Singapore. https://doi.org/10.1007/978-981-19-8307-8_4

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