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Showing: 51 - 100 of 250 results

  12. pAAV-TRE-GCaMP6s

    Plasmid
    #135418
    Purpose
    Can be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.
    Depositor
    Rylan Larsen
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Availability
    Academic Institutions and Nonprofits only
    135418-plasmid-map-sequence-id-270731

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  13. pAS1-his-24::GCaMP6s

    Plasmid
    #124333
    Purpose
    Expressing Nuclear Localized GCaMP6s
    Depositor
    Andrew Leifer
    Insert
    GCaMP6s (his-24 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    124333-plasmid-map-sequence-id-241917

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  21. DF-UAS-spa-GCaMP6f

    Plasmid
    #67568
    Purpose
    Zebrafish expression of a photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    spa-GCaMP6f
    Use
    Zebrafish transgenesis (see :a spinal opsin contr…
    Tags
    HIS-tag
    Expression
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  22. DF-UAS-sspa-GCaMP6f

    Plasmid
    #67569
    Purpose
    Zebrafish expression of a short photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    sspa-GCaMP6f
    Use
    Zebrafish transgenesis (see :a spinal opsin contr…
    Tags
    Expression
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  38. pAAV-UBC-S1-GCaMP6f

    Plasmid
    #160722
    Purpose
    Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.
    Depositor
    Edward Boyden
    Article
    Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31
    Insert
    S1-GCaMP6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Human ubiquitin C (UBC) promoter
    Availability
    Academic Institutions and Nonprofits only
    160722-plasmid-map-sequence-id-315521

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  39. pRSET a sfGCaMP6s-T78H

    Plasmid
    #100023
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutation
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s-T78H
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    100023-plasmid-map-sequence-id-194290

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  42. CMV-mt4xi-GCaMP6f

    Plasmid
    #192075
    Purpose
    Fluorescent reporter for mitochondrial calcium
    Depositor
    Timothy Ryan
    Insert
    4 repeats of improved COX8 signal peptide plus GCaMP6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    192075-plasmid-map-sequence-id-378919

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  44. pZac2.1 gfaABC1D-lck-GCaMP6f

    Plasmid
    #52924
    Purpose
    Fluorescent reporter for calcium signaling
    Depositor
    Baljit Khakh
    Has Service
    AAV5
    Insert
    lck-GCaMP6f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    52924-plasmid-map-sequence-id-126681

    Enlarge

  50. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid
    #105714
    Purpose
    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.
    Depositor
    Rylan Larsen
    Has Service
    AAV8
    Insert
    GCaMP6s
    Use
    AAV; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Availability
    Academic Institutions and Nonprofits only
    105714-plasmid-map-sequence-id-207265

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Showing: 51 - 100 of 250 results