Flow cytometric analysis of PDX-1 in mouse pancreatic tumor (insulinoma) cells. Beta-TC-6 (ATCC CRL-11506™) cells were fixed with BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). The cells were stained with either PE Mouse IgG1, κ isotype control (dashed line, Cat. No. 554680) or PE Mouse anti-PDX-1 monoclonal antibody (solid line, Cat. No. 562161) at matched concentrations. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885).
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Flow cytometric analysis of PDX-1 in mouse pancreatic tumor (insulinoma) cells. Beta-TC-6 (ATCC CRL-11506™) cells were fixed with BD Cytofix™ fixation buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). The cells were stained with either PE Mouse IgG1, κ isotype control (dashed line, Cat. No. 554680) or PE Mouse anti-PDX-1 monoclonal antibody (solid line, Cat. No. 562161) at matched concentrations. Flow cytometry was performed on a BD LSRFortessa™ flow cytometry system. This antibody has been found to be reactive with both mouse and human PDX-1, and it can also be used on fixed cells permeabilized with BD Phosflow™ Perm/Wash Buffer I (Cat. No. 557885).
Product Details
BD Pharmingen™
PDX1, GSF, IPF-1, IPF1, IUF-1, IDX-1, STF-1
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG1, κ
Human PDX-1 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
3651
AB_10893589
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
Perm/Wash Buffer I RUO
Size
125 mL
Cat No.
557885
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
562161 Rev. 1
Antibody Details
658A5
The 658A5 monoclonal antibody binds PDX-1 or Pancreas/Duodenum Homeobox Protein-1 encoded by the PDX1 or IPF1 (Insulin Promoter Factor 1) gene. PDX-1 is a key regulator of pancreatic development and adult beta-cell function. Loss of PDX1 gene function in mice and humans results in pancreatic agenesis. PDX-1 is also involved in endocrine precursor cell development by binding the DNA-binding transcription factor Neurogenin-3. PDX-1-positive cells have been obtained by the directed differentiation of human embryonic stem cells through a definitive endoderm lineage.
562161 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
562161 Rev.1
Citations & References
Development References (5)
-
Babu DA, Deering TG, Mirmira RG. A feat of metabolic proportions: Pdx1 orchestrates islet development and function in the maintenance of glucose homeostasis. Mol Genet Metab. 2007; 92(1-2):43-55. (Biology). View Reference
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D'Amour KA, Bang AG, Eliazer S, et al . Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol. 2006; 24(12):1481-1483. (Biology). View Reference
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Gannon M, Ables ET, Crawford L, et al. pdx-1 function is specifically required in embryonic beta cells to generate appropriate numbers of endocrine cell types and maintain glucose homeostasis. Dev Biol. 2008; 314(2):406-417. (Biology). View Reference
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Gu G, Dubauskaite J, Melton DA. Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Development. 2002; 129(10):2447-2457. (Biology). View Reference
-
Ohlsson H, Karlsson K, Edlund T. IPF1, a homeodomain-containing transactivator of the insulin gene. EMBO J. 1993; 12(11):4251-4259. (Biology). View Reference
562161 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
