Abstract
Neocamarosporium aquaticum, collected from a drift dead stem of a halophyte at the Kench Nature Reserve, Hayling Island, UK, is described as a new species in the family Neocamarosporiaceae, Dothideomycetes. Multi-locus analyses based on LSU, SSU, ITS rDNA, and EF1-α placed the new fungus as a distinct species in the genus Neocamarosporium, a sister to Neocamarosporium aesturinum isolated from seawater. The new species is characterized by the asexual morph with pycnidial conidiomata, doliiform, enteroblastic, annellidic conidiogenous cells, and conidia that are initially hyaline, aseptate, with longitudinal and transverse septa when matured, muriform, and turning brown to dark brown on maturity. Neocamarosporium aquaticum and Ne. aesturinum differ in the colour of the conidia, degree of septation, and conidial measurements.
About the authors
Chasika Prematunga is pursuing his PhD degree in Biological Sciences at the Center of Excellence in Fungal Research, Mae Fah Luang University (Thailand) under the supervision of Prof. Dr. Kevin D. Hyde. He works on the taxonomy and phylogeny of aquatic fungi.
Saranyaphat Boonmee obtained a PhD in Biosciences from the Center of Excellence in Fungal Research, Mae Fah Luang University in 2014. She is a prolific researcher with 88 SCI papers and with an H-index of 37. She is a lecturer in the School of Science, Mae Fah Luang University (Thailand).
E. B. Gareth Jones is a widely cited author on marine fungi, their ecology, physiology, and systematics and has co-edited several books and journal special issues. He has reported on marine fungi from around the world, in particular Asia, especially those found on mangroves. He supervised over 100 Ph.D. and is a highly cited scientist.
Mark S. Calabon obtained a PhD (Biological Sciences) degree from the Center of Excellence in Fungal Research, Mae Fah Luang University (Thailand). He is an assistant professor in the University of the Philippines Visayas. His research focuses on the taxonomy, phylogeny, and classification of aquatic fungi.
Acknowledgements
Chasika Prematunga and Mark S. Calabon are grateful to Prof. Kevin D. Hyde for invaluable inputs in the conduct of the research.
-
Author contributions: The study conception and design were performed by Chasika Prematunga and E. B. Gareth Jones. All authors contributed to material preparation, data collection and analysis. The first draft of the manuscript was written by Chasika Prematunga and E. B. Gareth Jones, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
-
Research funding: E. B. Gareth Jones is supported under the Distinguished Scientist Fellowship Program (DSFP), King Saud University, Kingdom of Saudi Arabia.
-
Conflict of interest statement: The authors declare that they have no conflicts of interest regarding this article.
-
Data availability: The datasets analyzed during the current study are available from the corresponding author on reasonable request. All sequences generated here are available from GenBank with accession numbers: OP730890, OP730894 (ITS); OP730885, OP730886 (LSU); OP730891, OP730892 (SSU).
References
Calabon, M.S., Jones, E.B.G., Promputtha, I., and Hyde, K.D. (2021). Fungal biodiversity in salt marsh ecosystems. J. Fungi 7: 648, https://doi.org/10.3390/jof7080648.Search in Google Scholar PubMed PubMed Central
Choi, Y.W., Hyde, K.D., and Ho, W.W.H. (1999). Single spore isolation of fungi. Fungal Divers. 3: 29–38.Search in Google Scholar
Crous, P.W., Shivas, R.G., Quaedvlieg, W., van der Bank, M., Zhang, Y., Summerell, B.A., Guarro, J., Wingfield, M.J., Wood, A.R., Alfenas, A.C., et al.. (2014). Fungal Planet description sheets: 214–280. Persoonia 32: 184–306, https://doi.org/10.3767/003158514x682395.Search in Google Scholar PubMed PubMed Central
Darriba, D., Taboada, G.L., Doallo, R., and Posada, D. (2012). JModelTest 2: more models, new heuristics and parallel computing. Nat. Methods 9: 772, https://doi.org/10.1038/nmeth.2109.Search in Google Scholar PubMed PubMed Central
Dayarathne, M.C., Jones, E.B.G., Maharachchikumbura, S.S.N., Devadatha, B., Sarma, V.V., Khongphinitbunjong, K., Chomnunti, P., and Hyde, K.D. (2020). Morpho-molecular characterization of microfungi associated with marine based habitats. Mycosphere 11: 1–188, https://doi.org/10.5943/mycosphere/11/1/1.Search in Google Scholar
Devadatha, B., Jones, E.B.G., Pang, K.L., Abdel-Wahab, M.A., Hyde, K.D., Sakayaroj, J., Bahkali, A.H., Calabon, M.S., Sarma, V.V., Sutreong, S., et al.. (2021). Occurrence and geographical distribution of mangrove fungi. Fungal Divers. 106: 137–227, https://doi.org/10.1007/s13225-020-00468-0.Search in Google Scholar
Glez-Peña, D., Gómez-Blanco, D., Reboiro-Jato, M., Fdez-Riverola, F., and Posada, D. (2010). ALTER: program-oriented conversion of DNA and protein alignments. Nucleic Acids Res. 38: W14–W18.10.1093/nar/gkq321Search in Google Scholar PubMed PubMed Central
Gonçalves, M.F.M., Aleixo, A., Vicente, T.F.L., Esteves, A.C., and Alves, A. (2019). Three new species of Neocamarosporium isolated from saline environments: N. aestuarinum sp. nov., N. endophyticum sp. nov. and N. halimiones sp. nov. Mycosphere 10: 608–621.10.5943/mycosphere/10/1/11Search in Google Scholar
Hagestad, O.C., Hou, L., Andersen, J.H., Hansen, E.H., Altermark, B., Li, C., Kuhnert, E., Cox, R.J., Crous, P.W., Spatafora, J.W., et al.. (2021). Genomic characterization of three marine fungi, including Emericellopsis atlantica sp. nov. with signatures of a generalist lifestyle and marine biomass degradation. IMA Fungus 12: 21, https://doi.org/10.1186/s43008-021-00072-0.Search in Google Scholar PubMed PubMed Central
Hall, T.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41: 95–98.Search in Google Scholar
He, C., Han, T., Tan, L., and Li, X. (2022). Effects of dark septate endophytes on the performance and soil microbia of Lycium ruthenicum under drought stress. Front. Plant Sci. 13: 898378, https://doi.org/10.3389/fpls.2022.898378.Search in Google Scholar PubMed PubMed Central
Hyde, K.D. and Soytong, K. (2008). The fungal endophyte dilemma. Fungal Divers. 33: 163–173.Search in Google Scholar
Index Fungorum. (2022a). Index Fungorum – register a name or other nomenclatural act, <http://www.indexfungorum.org/names/IndexFungorumRegisterName.asp> (Accessed 30 October 2022).Search in Google Scholar
Index Fungorum. (2022b). Index Fungorum, <http://www.indexfungorum.org/names/Names.asp> (Accessed 11 October 2022).Search in Google Scholar
Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., et al.. (2015). The Faces of Fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Divers. 74: 3–18, https://doi.org/10.1007/s13225-015-0351-8.Search in Google Scholar
Jones, E.B.G., Ramakrishna, S., Vikineswary, S., Das, D., Bah, A.H., and Pang, K.L. (2022). How do fungi survive in the sea and respond to climate change? J. Fungi 8: 291–310, https://doi.org/10.3390/jof8030291.Search in Google Scholar PubMed PubMed Central
Katoh, K., Rozewicki, J., and Yamada, K.D. (2019). MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings Bioinf. 20: 1160–1166, https://doi.org/10.1093/bib/bbx108.Search in Google Scholar PubMed PubMed Central
Miller, M.A., Pfeiffer, W., and Schwartz, T. (2010). Creating the CIPRES science gateway for inference of large phylogenetic trees. In: Gateway computing environments workshop, GCE 2010. Institute of Electrical and Electronics Engineers (IEEE), New Orleans, pp. 1–8.10.1109/GCE.2010.5676129Search in Google Scholar
Nylander, J.A.A. (2004). MrModeltest 2.0. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala.Search in Google Scholar
Papizadeh, M., Wijayawardene, N.N., Amoozegar, M.A., Saba, F., Fazeli, S.A.S., and Hyde, K.D. (2018). Neocamarosporium jorjanensis, N. persepolisi, and N. solicola spp. nov. (Neocamarosporiaceae, Pleosporales) isolated from saline lakes of Iran indicate the possible halotolerant nature for the genus. Mycol. Prog. 17: 661–679, https://doi.org/10.1007/s11557-017-1341-x.Search in Google Scholar
Ronquist, F. and Huelsenbeck, J.P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574, https://doi.org/10.1093/bioinformatics/btg180.Search in Google Scholar PubMed
Stamatakis, A. (2006). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690, https://doi.org/10.1093/bioinformatics/btl446.Search in Google Scholar PubMed
Stamatakis, A. (2014). RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312–1313, https://doi.org/10.1093/bioinformatics/btu033.Search in Google Scholar PubMed PubMed Central
Stamatakis, A., Hoover, P., and Rougemont, J. (2008). A rapid bootstrap algorithm for the RAxML web servers. Syst. Biol. 57: 758–771, https://doi.org/10.1080/10635150802429642.Search in Google Scholar PubMed
Vaghefi, N., Silva, A., Koenick, L.B., and Pethybridge, S.J. (2019). Genome resource for Neocamarosporium betae (syn. Pleospora betae), the cause of Phoma leaf spot and root rot on Beta vulgaris. Mol. Plant Microbe Interact. 32: 787–789, https://doi.org/10.1094/mpmi-12-18-0334-a.Search in Google Scholar PubMed
Wanasinghe, D.N., Hyde, K.D., Jeewon, R., Crous, P.W., Wijayawardene, N.N., Jones, E.B.G., Bhat, D.J., Phillips, A.J.L., Groenewald, J.Z., Dayarathne, M.C., et al.. (2017). Phylogenetic revision of Camarosporium (Pleosporineae, Dothideomycetes) and allied genera. Stud. Mycol. 87: 207–256, https://doi.org/10.1016/j.simyco.2017.08.001.Search in Google Scholar PubMed PubMed Central
White, T., Bruns, T., Lee, S., and Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J. (Eds.), PCR protocols, a guide to methods and applications. San Diego: Academic Press, pp. 315–322.10.1016/B978-0-12-372180-8.50042-1Search in Google Scholar
Yin, H., Zhou, J., Lv, H., Qin, N., Chang, F.J., and Zhao, X. (2020). Identification, pathogenicity, and fungicide sensitivity of Ascochyta caulina (Teleomorph: Neocamarosporium calvescens) associated with black stem on quinoa in China. Plant Dis. 104: 2585–2597, https://doi.org/10.1094/pdis-09-19-2042-re.Search in Google Scholar
© 2023 Walter de Gruyter GmbH, Berlin/Boston
