1-Iodohexadecane Alleviates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in Mice: Possible Involvements of the Skin Barrier and Mast Cell SNARE Proteins
Abstract
:1. Introduction
2. Results
2.1. Effects of 1-Iodohexadecane on the Expressions of SNARE Proteins in Mast Cells
2.2. Effects of 1-Iodohexadecane on Histamine and β-Hexosaminidase Secretion by Mast Cells
2.3. Effects of 1-Iodohexadecane on Skin Barrier Protein Expressions in Human Keratinocytes
2.4. Effect of 1-Iodohexadecane on Skin Lesions in the DNCB-Induced Murine Model
2.5. Histopathological Alterations and Serum Levels of Mast Cell Degranulation Markers in the DNCB-Induced Murine Model Exposed to 1-Iodohexadecane
2.6. Effect of 1-Iodohexadecane on Skin Barrier Proteins in the DNCB-Induced Murine Model
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Cell Viability
4.4. Immunoblotting
4.5. β-Hexosaminidase and Histamine Release Assays
4.6. DNCB-Induced Atopic Dermatitis Animal Model
4.7. Evaluation of Dermatitis Severity
4.8. Histopathological Analysis
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Kim, D.Y.; Won, K.-J.; Hwang, D.I.; Kim, N.Y.; Kim, B.; Lee, H.M. 1-Iodohexadecane Alleviates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in Mice: Possible Involvements of the Skin Barrier and Mast Cell SNARE Proteins. Molecules 2022, 27, 1560. https://doi.org/10.3390/molecules27051560
Kim DY, Won K-J, Hwang DI, Kim NY, Kim B, Lee HM. 1-Iodohexadecane Alleviates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in Mice: Possible Involvements of the Skin Barrier and Mast Cell SNARE Proteins. Molecules. 2022; 27(5):1560. https://doi.org/10.3390/molecules27051560
Chicago/Turabian StyleKim, Do Yoon, Kyung-Jong Won, Dae Il Hwang, Nan Young Kim, Bokyung Kim, and Hwan Myung Lee. 2022. "1-Iodohexadecane Alleviates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis in Mice: Possible Involvements of the Skin Barrier and Mast Cell SNARE Proteins" Molecules 27, no. 5: 1560. https://doi.org/10.3390/molecules27051560